ID SELU_PSESM Reviewed; 366 AA.
AC Q889F6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 01-MAY-2013, entry version 63.
DE RecName: Full=tRNA 2-selenouridine synthase;
DE EC=2.9.1.-;
DE AltName: Full=Selenophosphate-dependent tRNA 2-selenouridine synthase;
GN Name=selU; OrderedLocusNames=PSPTO_0794;
OS Pseudomonas syringae pv. tomato (strain DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F.,
RA Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H.,
RA Nelson W.C., Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q.,
RA Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J.,
RA Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M.,
RA Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K.,
RA Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X.,
RA Bender C.L., White O., Fraser C.M., Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Catalyzes the transfer of selenium from selenophosphate
CC for conversion of 2-thiouridine to 2-selenouridine at the wobble
CC position in tRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: 5-methylaminomethyl-2-thiouridine +
CC selenophosphate = 5-methylaminomethyl-2-selenouridine + phosphate.
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SIMILARITY: Belongs to the 2-selenouridine synthase family.
CC -!- SIMILARITY: Contains 1 rhodanese domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AE016853; AAO54336.1; -; Genomic_DNA.
DR RefSeq; NP_790641.1; NC_004578.1.
DR ProteinModelPortal; Q889F6; -.
DR STRING; 223283.PSPTO_0794; -.
DR DNASU; 1182419; -.
DR EnsemblBacteria; AAO54336; AAO54336; PSPTO_0794.
DR GeneID; 1182419; -.
DR KEGG; pst:PSPTO_0794; -.
DR PATRIC; 19992789; VBIPseSyr93040_0807.
DR eggNOG; COG2603; -.
DR HOGENOM; HOG000260000; -.
DR KO; K06917; -.
DR OMA; NLPLMND; -.
DR ProtClustDB; PRK11784; -.
DR BioCyc; PSYR223283:GJIX-806-MONOMER; -.
DR GO; GO:0043828; F:tRNA 2-selenouridine synthase activity; IEA:InterPro.
DR GO; GO:0070329; P:tRNA seleno-modification; IEA:InterPro.
DR Gene3D; 3.40.250.10; -; 1.
DR HAMAP; MF_01622; tRNA_sel_U_synth; 1; -.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR017582; tRNA_2-selenouridine_synthase.
DR SMART; SM00450; RHOD; 1.
DR SUPFAM; SSF52821; Rhodanese-like; 1.
DR TIGRFAMs; TIGR03167; tRNA_sel_U_synt; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 3: Inferred from homology;
KW Complete proteome; Selenium; Transferase.
FT CHAIN 1 366 tRNA 2-selenouridine synthase.
FT /FTId=PRO_0000210869.
FT DOMAIN 12 135 Rhodanese.
FT ACT_SITE 95 95 S-selanylcysteine intermediate (By
FT similarity).
SQ SEQUENCE 366 AA; 41080 MW; 534463FF7DF13A1F CRC64;
MADNSSDYRA LFLNDVPMMD ARAPVEFSKG AFPGVINLPL MNDIERQKVG TCYKQHGQDA
AIQLGHQLVC GQVKDERVEA WVEFARANPN GYLYCFRGGL RSQTVQRWLK DAGVDYPRIL
GGYKAMRTFL LDTLHEAVTE CDLVVLGGMT GTGKTEVLTQ LPNGLDLEGV ANHRGSSFGK
RATGQPAQID FENRLAINLL KKRAAGIEQF VVEDESRLVG SCNVPLELHQ AMQGCPVVWL
EDSFEHRVER ILADYVVNLC AEFISVKGEE LGFGLFADRL LQSLNNIHKR LGGERHQRLL
ALMQTALEEQ QRSGAVELHR GWIEGLLGEY YDPMYAYQRE HKAARIEFAG NQFEVSGYLS
ERSSRR
//
