ID Q88N06_PSEPK Unreviewed; 368 AA.
AC Q88N06;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=3-hydroxyisobutyryl-CoA hydrolase {ECO:0000256|ARBA:ARBA00011915};
DE EC=3.1.2.4 {ECO:0000256|ARBA:ARBA00011915};
GN OrderedLocusNames=PP_1412 {ECO:0000313|EMBL:AAN67034.1};
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488 {ECO:0000313|EMBL:AAN67034.1, ECO:0000313|Proteomes:UP000000556};
RN [1] {ECO:0000313|EMBL:AAN67034.1, ECO:0000313|Proteomes:UP000000556}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440
RC {ECO:0000313|Proteomes:UP000000556};
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L., Beanan M., DeBoy R.T., Daugherty S., Kolonay J., Madupu R.,
RA Nelson W., White O., Peterson J., Khouri H., Hance I., Chris Lee P.,
RA Holtzapple E., Scanlan D., Tran K., Moazzez A., Utterback T., Rizzo M.,
RA Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D.,
RA Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N.,
RA Dusterhoft A., Tummler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-
CC methylpropanoate + CoA + H(+); Xref=Rhea:RHEA:20888,
CC ChEBI:CHEBI:11805, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57340; EC=3.1.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001709};
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DR EMBL; AE015451; AAN67034.1; -; Genomic_DNA.
DR RefSeq; NP_743570.1; NC_002947.4.
DR RefSeq; WP_010952519.1; NC_002947.4.
DR AlphaFoldDB; Q88N06; -.
DR STRING; 160488.PP_1412; -.
DR PaxDb; 160488-PP_1412; -.
DR GeneID; 83682053; -.
DR KEGG; ppu:PP_1412; -.
DR PATRIC; fig|160488.4.peg.1498; -.
DR eggNOG; COG1024; Bacteria.
DR HOGENOM; CLU_009834_22_1_6; -.
DR OrthoDB; 9790967at2; -.
DR PhylomeDB; Q88N06; -.
DR BioCyc; PPUT160488:G1G01-1504-MONOMER; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR CDD; cd06558; crotonase-like; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR045004; ECH_dom.
DR InterPro; IPR032259; HIBYL-CoA-H.
DR PANTHER; PTHR43176:SF3; 3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43176; 3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED; 1.
DR Pfam; PF16113; ECH_2; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:AAN67034.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000556}.
FT DOMAIN 16..354
FT /note="Enoyl-CoA hydratase/isomerase"
FT /evidence="ECO:0000259|Pfam:PF16113"
SQ SEQUENCE 368 AA; 40490 MW; 4D019E9165EBA300 CRC64;
MTIHCEVLTG VDGARIGIAT LDAPKALNAL NLPMIEVLGE QLHAWARDPG IVCVLLRGNG
AKAFCAGGDV RALAQACRDH PGSVPPLAAS FFAAEYRLDF ALHTYPKPLL CWGHGHVLGG
GMGLLQGANV RIVTPSSRLA MPEISIGLYP DVGASWFLAR LPGKLGLFFG LTGAPINARD
ALDLGLADRF LGEHQQEALI EELLQLNWQE QTAIQLNSLL KAEQHRACAE LPDAQWLPRR
HEIDQLLDVA DAASAWRALE RLKQHDDPLL ADAGHRLHEG CPLTAHLVWE QIRRARYLSL
AQVFQMEYSM SLNCCRHPEF SEGVRARLLD KDNQPRWHWP DVAQVPTAVV EAHFAKAWEG
RHPLADVG
//
