ID Q89K07_BRADU Unreviewed; 780 AA.
AC Q89K07;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 130.
DE RecName: Full=Ribonuclease R {ECO:0000256|HAMAP-Rule:MF_01895};
DE Short=RNase R {ECO:0000256|HAMAP-Rule:MF_01895};
DE EC=3.1.13.1 {ECO:0000256|HAMAP-Rule:MF_01895};
GN Name=rnr {ECO:0000256|HAMAP-Rule:MF_01895,
GN ECO:0000313|EMBL:BAC50377.1};
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911 {ECO:0000313|EMBL:BAC50377.1, ECO:0000313|Proteomes:UP000002526};
RN [1] {ECO:0000313|Proteomes:UP000002526}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110
RC {ECO:0000313|Proteomes:UP000002526};
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- FUNCTION: 3'-5' exoribonuclease that releases 5'-nucleoside
CC monophosphates and is involved in maturation of structured RNAs.
CC {ECO:0000256|HAMAP-Rule:MF_01895}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC nucleoside 5'-phosphates.; EC=3.1.13.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001849, ECO:0000256|HAMAP-
CC Rule:MF_01895};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01895}.
CC -!- SIMILARITY: Belongs to the RNR ribonuclease family. RNase R subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_01895}.
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DR EMBL; BA000040; BAC50377.1; -; Genomic_DNA.
DR RefSeq; NP_771752.1; NC_004463.1.
DR RefSeq; WP_011087872.1; NZ_CP011360.1.
DR AlphaFoldDB; Q89K07; -.
DR STRING; 224911.AAV28_22930; -.
DR EnsemblBacteria; BAC50377; BAC50377; BAC50377.
DR GeneID; 64024875; -.
DR KEGG; bja:blr5112; -.
DR PATRIC; fig|224911.44.peg.4981; -.
DR eggNOG; COG0557; Bacteria.
DR HOGENOM; CLU_002333_7_1_5; -.
DR InParanoid; Q89K07; -.
DR OrthoDB; 9764149at2; -.
DR PhylomeDB; Q89K07; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008859; F:exoribonuclease II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016070; P:RNA metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04471; S1_RNase_R; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR HAMAP; MF_01895; RNase_R; 1.
DR InterPro; IPR040476; CSD2.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR001900; RNase_II/R.
DR InterPro; IPR022966; RNase_II/R_CS.
DR InterPro; IPR004476; RNase_II/RNase_R.
DR InterPro; IPR011805; RNase_R.
DR InterPro; IPR003029; S1_domain.
DR NCBIfam; TIGR00358; 3_prime_RNase; 1.
DR NCBIfam; TIGR02063; RNase_R; 1.
DR PANTHER; PTHR23355:SF9; DIS3-LIKE EXONUCLEASE 2; 1.
DR PANTHER; PTHR23355; RIBONUCLEASE; 1.
DR Pfam; PF17876; CSD2; 1.
DR Pfam; PF00773; RNB; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00955; RNB; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 2.
DR PROSITE; PS01175; RIBONUCLEASE_II; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01895};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_01895};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01895};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01895};
KW Reference proteome {ECO:0000313|Proteomes:UP000002526};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01895}.
FT DOMAIN 641..722
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT REGION 725..780
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 738..780
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 780 AA; 85628 MW; 5D8AA6FE99791203 CRC64;
MKRKNDRGFP DRQAIVAFIK ANQGNVGTRE IAREFGLKNA DRVELKRMLR ELADDGTIKK
KRHKVSEPDT LPSTLVADIT GRDSDGELIA SPTEWDEVES GEPPKILIVM PRRPKPGTAA
GVGDRALLRV ERIDETEGPA YRGHIIKVFD KAKSRILGVF RSLPEGGGRL VPVDKKSADR
ELNIAAADTQ GAQDGDLVSV DIVRSRAFGL ASGRVKEKLG SVKSEKAISL IAIYAHDIPL
QFRPAAEREA EAAEPANLKG REDWRDVPLV TIDPPDAKDH DDAVHAQADD DPNNKGGFIV
NVAIADVSFY VRPGTALDRD ALDRGNSVYF PDRVVPMLPE RISNNLCSLV PGEPRGALAV
RMVIGPDGRK RSHTFHRILM RSAAKLNYAQ AQAAIDGRPD DTTGPLLDPI LRPLYTAYAC
VKRARDERDP LNLDLPERKI LLKSDGTVDR VIVPERLDAH KLIEEFMILA NVAAAEMLEK
KSLPLIYRVH DEPTLEKVHA LAEFLETLDV PFAKSGALRP ALFNRVLAQL EGHDHFPLVS
EVVLRAQAQA EYSAENYGHF GLNLRRYAHF TSPIRRYADL VVHRALVRAL GLGEGALPDT
ETPETLGEVA AHISVTERRA MKAERETVDR LIAHHLADRI GSSFQGRVSG VTRAGLFVKL
SETGADGLIP IRSLGTEYFN YDESRHALVG TRSGAMHQLG DVVDVRLIEA APIAGALRFE
LLSSSGETAP QGRRASGRQR RPSFKAHPGR SPAKKHKPAK PKKPGKGKKN KGKGQKGKAW
//
