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Database: UniProt
Entry: Q8D335
LinkDB: Q8D335
Original site: Q8D335 
ID   GSHB_WIGBR              Reviewed;         316 AA.
AC   Q8D335;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 2.
DT   01-OCT-2014, entry version 65.
DE   RecName: Full=Glutathione synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSH-S {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSHase {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000255|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000255|HAMAP-Rule:MF_00162};
GN   OrderedLocusNames=WIGBR1660;
OS   Wigglesworthia glossinidia brevipalpis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Wigglesworthia.
OX   NCBI_TaxID=36870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12219091; DOI=10.1038/ng986;
RA   Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M.,
RA   Aksoy S.;
RT   "Genome sequence of the endocellular obligate symbiont of tsetse
RT   flies, Wigglesworthia glossinidia.";
RL   Nat. Genet. 32:402-407(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione. {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit.
CC       {ECO:0000250}.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC24312.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000021; BAC24312.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_871169.1; NC_004344.2.
DR   ProteinModelPortal; Q8D335; -.
DR   STRING; 36870.WGLp166; -.
DR   EnsemblBacteria; BAC24312; BAC24312; BAC24312.
DR   GeneID; 1256961; -.
DR   KEGG; wbr:WGLp166; -.
DR   PATRIC; 24024487; VBIWigGlo15804_0186.
DR   eggNOG; COG0189; -.
DR   KO; K01920; -.
DR   OMA; DWKIARA; -.
DR   OrthoDB; EOG6WMHWB; -.
DR   UniPathway; UPA00142; UER00210.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    316       Glutathione synthetase.
FT                                /FTId=PRO_0000197495.
FT   DOMAIN      123    309       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_00162}.
FT   NP_BIND     149    206       ATP. {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       280    280       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       282    282       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
SQ   SEQUENCE   316 AA;  36507 MW;  B54026AC2A0E7F44 CRC64;
     MIKLGIIMDP ISSIKIKKDT SFSIMLEAQK RNYFLYYIEM KDIYYEVGEV YANSYLISVK
     YDEKKWYSFK KKYTINLKEL DVILMRKDPP FDIEFLYITY ILEHIENFGV LIINKPKSLR
     DYNEKISTLS FKYSPKTLIS CSKKAIYSFQ EKFGDIILKP INKMGGDSVF YVKKNDPNVS
     VIIDQLTNYG NSFCLIQEYI KEILNGDRRI IMINGSPLPY CLVRIPNDKE IRGNLAAGAS
     FDILPLRKID YEISNNISSF LKDKGLIFVG LDIIGNYLTE INITSPTGIN EIESVYKVSI
     SGILLDSIEK LLNIKH
//
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