ID PYRDA_STRR6 Reviewed; 311 AA.
AC Q8DQG9;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 01-MAY-2013, entry version 75.
DE RecName: Full=Probable dihydroorotate dehydrogenase A (fumarate);
DE Short=DHOD A;
DE Short=DHODase A;
DE Short=DHOdehase A;
DE EC=1.3.98.1;
GN Name=pyrDA; OrderedLocusNames=spr0672;
OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=171101;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=11544234; DOI=10.1128/JB.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E.,
RA LeBlanc D.J., Lee L.N., Lefkowitz E.J., Lu J., Matsushima P.,
RA McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I.,
RA Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P.,
RA Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G.,
RA Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L.,
RA Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
CC -!- FUNCTION: Catalyzes the conversion of dihydroorotate to orotate
CC with fumarate as the electron acceptor (By similarity).
CC -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + fumarate = orotate +
CC succinate.
CC -!- COFACTOR: Binds 1 FMN per subunit (By similarity).
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC pathway.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the dihydroorotate dehydrogenase family.
CC Type 1 subfamily.
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DR EMBL; AE007317; AAK99476.1; -; Genomic_DNA.
DR PIR; H97955; H97955.
DR RefSeq; NP_358266.1; NC_003098.1.
DR ProteinModelPortal; Q8DQG9; -.
DR SMR; Q8DQG9; 6-308.
DR STRING; 171101.spr0672; -.
DR EnsemblBacteria; AAK99476; AAK99476; spr0672.
DR GeneID; 934210; -.
DR KEGG; spr:spr0672; -.
DR PATRIC; 19701243; VBIStrPne107296_0744.
DR eggNOG; COG0167; -.
DR HOGENOM; HOG000225104; -.
DR KO; K00226; -.
DR OMA; QAAAVFN; -.
DR ProtClustDB; PRK02506; -.
DR UniPathway; UPA00070; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052888; F:dihydroorotate oxidase (fumarate) activity; IEA:EC.
DR GO; GO:0004158; F:dihydroorotate oxidase activity; IEA:InterPro.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00224; DHO_dh_type1; 1; -.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR005720; Dihydroorotate_DH.
DR InterPro; IPR024920; Dihydroorotate_DH_1.
DR InterPro; IPR012135; Dihydroorotate_DH_1_2.
DR InterPro; IPR001295; Dihydroorotate_DH_CS.
DR Pfam; PF01180; DHO_dh; 1.
DR PIRSF; PIRSF000164; DHO_oxidase; 1.
DR TIGRFAMs; TIGR01037; pyrD_sub1_fam; 1.
DR PROSITE; PS00911; DHODEHASE_1; 1.
DR PROSITE; PS00912; DHODEHASE_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Flavoprotein; FMN; Oxidoreductase;
KW Pyrimidine biosynthesis; Reference proteome.
FT CHAIN 1 311 Probable dihydroorotate dehydrogenase A
FT (fumarate).
FT /FTId=PRO_0000148403.
FT NP_BIND 45 46 FMN (By similarity).
FT NP_BIND 248 249 FMN (By similarity).
FT NP_BIND 270 271 FMN (By similarity).
FT REGION 69 73 Substrate binding (By similarity).
FT REGION 194 195 Substrate binding (By similarity).
FT ACT_SITE 131 131 Nucleophile.
FT BINDING 45 45 Substrate (By similarity).
FT BINDING 128 128 FMN (By similarity).
FT BINDING 128 128 Substrate (By similarity).
FT BINDING 165 165 FMN (By similarity).
FT BINDING 193 193 FMN; via carbonyl oxygen (By similarity).
FT BINDING 220 220 FMN; via amide nitrogen (By similarity).
SQ SEQUENCE 311 AA; 34501 MW; E00F94D5FCFEC3F4 CRC64;
MVSTKTQIAG FEFDNCLMNA AGVACMTIEE LEEVKNSAAG TFVTKTATLD FRQGNPEPRY
QDVPLGSINS MGLPNNGLDY YLDYLLDLQE KESNRTFFLS LVGMSPEETH TILKKVQESD
FRGLTELNLS CPNVPGKPQI AYDFETTDRI LAEVFAYFTK PLGIKLPPYF DIVHFDQAAA
IFNKYPLKFV NCVNSSGNGL YIEDESVVIR PKNGFGGIGG EYIKPTALAN VHAFYQRLNP
QIQIIGTGGV LTGRDAFEHI LCGASMVQVG TTLHKEGVSA FDRITNELKA IMVEKGYESL
EDFRGKLRYI D
//
