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Database: UniProt
Entry: Q8DZX5
LinkDB: Q8DZX5
Original site: Q8DZX5 
ID   TRMFO_STRA5             Reviewed;         444 AA.
AC   Q8DZX5;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   14-MAY-2014, entry version 74.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO;
DE            EC=2.1.1.74;
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase;
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;
GN   Name=trmFO; Synonyms=gid; OrderedLocusNames=SAG0969;
OS   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=208435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-611 / 2603 V/R;
RX   PubMed=12200547; DOI=10.1073/pnas.182380799;
RA   Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N.,
RA   Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA   Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R.,
RA   Radune D., Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S.,
RA   Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M.,
RA   Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D.,
RA   Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G.,
RA   Fraser C.M.;
RT   "Complete genome sequence and comparative genomic analysis of an
RT   emerging human pathogen, serotype V Streptococcus agalactiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + uracil(54) in
CC       tRNA + FADH(2) = tetrahydrofolate + 5-methyluracil(54) in tRNA +
CC       FAD.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
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DR   EMBL; AE009948; AAM99853.1; -; Genomic_DNA.
DR   RefSeq; NP_687981.1; NC_004116.1.
DR   ProteinModelPortal; Q8DZX5; -.
DR   STRING; 208435.SAG0969; -.
DR   EnsemblBacteria; AAM99853; AAM99853; SAG0969.
DR   GeneID; 1013773; -.
DR   KEGG; sag:SAG0969; -.
DR   PATRIC; 19628768; VBIStrAga72745_0975.
DR   eggNOG; COG1206; -.
DR   HOGENOM; HOG000252054; -.
DR   KO; K04094; -.
DR   OMA; EIYFEGC; -.
DR   OrthoDB; EOG6J74VT; -.
DR   BioCyc; SAGA208435:GHVY-1060-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030698; F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01037; TrmFO; 1.
DR   InterPro; IPR004417; Folate-dep_Ribothymidyl_synth.
DR   InterPro; IPR002218; GIDA-rel.
DR   InterPro; IPR020595; GIDA-rel_CS.
DR   Pfam; PF01134; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Transferase; tRNA processing.
FT   CHAIN         1    444       Methylenetetrahydrofolate--tRNA-(uracil-
FT                                5-)-methyltransferase TrmFO.
FT                                /FTId=PRO_0000117270.
FT   NP_BIND      10     15       FAD (By similarity).
SQ   SEQUENCE   444 AA;  49511 MW;  CA438676C26001C4 CRC64;
     MSQSYINVIG AGLAGSEAAY QIAKRGIPVK LYEMRGVKST PQHKTDNFAE LVCSNSFRGD
     SLTNAVGLLK EEMRRLDSII MRNGEAHRVP AGGAMAVDRE GYSEAVTEEI HKHPLIEVIR
     DEITDIPGDA ITVIATGPLT SDSLAAKIHE LNGGDGFYFY DAAAPIVDKN TIDINKVYLK
     SRYDKGEAAY LNCPMTKEEF MAFHEALTTA EEAPLNSFEK EKYFEGCMPI EVMAKRGIKT
     MLYGPMKPVG LEYPEDYKGP RDGEFKTPYA VVQLRQDNAA GSLYNIVGFQ THLKWGEQKR
     VFQMIPGLEN AEFVRYGVMH RNSYMDSPNL LNQTFATRKN PNLFFAGQMT GVEGYVESAA
     SGLVAGINAV RRFNGESEVV FPQTTAIGAL PHYITHTDSK HFQPMNVNFG IIKELEGPRI
     RDKKERYEAI ATRALKDLEK FLNY
//
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