ID GLPK_BRUSU Reviewed; 498 AA.
AC Q8FWK8; G0KCH8;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 01-MAY-2013, entry version 68.
DE RecName: Full=Glycerol kinase;
DE EC=2.7.1.30;
DE AltName: Full=ATP:glycerol 3-phosphotransferase;
DE AltName: Full=Glycerokinase;
DE Short=GK;
GN Name=glpK; OrderedLocusNames=BRA0443, BS1330_II0440;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Brucellaceae; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A.,
RA Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O.,
RA Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M.,
RA Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between
RT animal and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/JB.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
CC -!- FUNCTION: Key enzyme in the regulation of glycerol uptake and
CC metabolism (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3-
CC phosphate.
CC -!- PATHWAY: Polyol metabolism; glycerol degradation via glycerol
CC kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
CC -!- SIMILARITY: Belongs to the FGGY kinase family.
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DR EMBL; AE014292; AAN33637.1; -; Genomic_DNA.
DR EMBL; CP002998; AEM19916.1; -; Genomic_DNA.
DR RefSeq; NP_699632.1; NC_004311.2.
DR RefSeq; YP_005614142.1; NC_017250.1.
DR ProteinModelPortal; Q8FWK8; -.
DR SMR; Q8FWK8; 4-494.
DR STRING; 204722.BRA0443; -.
DR EnsemblBacteria; AAN33637; AAN33637; BRA0443.
DR EnsemblBacteria; AEM19916; AEM19916; BS1330_II0440.
DR GeneID; 1164881; -.
DR GeneID; 12139875; -.
DR KEGG; bms:BRA0443; -.
DR KEGG; bsi:BS1330_II0440; -.
DR PATRIC; 17793689; VBIBruSui107850_2660.
DR eggNOG; COG0554; -.
DR HOGENOM; HOG000222134; -.
DR KO; K00864; -.
DR OMA; NWVMQFL; -.
DR ProtClustDB; CLSK863056; -.
DR UniPathway; UPA00618; UER00672.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004370; F:glycerol kinase activity; IEA:HAMAP.
DR GO; GO:0019563; P:glycerol catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:HAMAP.
DR HAMAP; MF_00186; Glycerol_kin; 1; -.
DR InterPro; IPR018485; Carb_kinase_FGGY_C.
DR InterPro; IPR018483; Carb_kinase_FGGY_CS.
DR InterPro; IPR018484; Carb_kinase_FGGY_N.
DR InterPro; IPR005999; Glycerol_kin.
DR PANTHER; PTHR10196:SF9; PTHR10196:SF9; 1.
DR Pfam; PF02782; FGGY_C; 1.
DR Pfam; PF00370; FGGY_N; 1.
DR TIGRFAMs; TIGR01311; glycerol_kin; 1.
DR PROSITE; PS00933; FGGY_KINASES_1; 1.
DR PROSITE; PS00445; FGGY_KINASES_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Glycerol metabolism; Kinase;
KW Nucleotide-binding; Transferase.
FT CHAIN 1 498 Glycerol kinase.
FT /FTId=PRO_0000059441.
FT NP_BIND 411 415 ATP (By similarity).
FT BINDING 12 12 Substrate (By similarity).
FT BINDING 16 16 ATP (By similarity).
FT BINDING 82 82 Substrate (By similarity).
FT BINDING 134 134 Substrate (By similarity).
FT BINDING 243 243 Substrate (By similarity).
FT BINDING 265 265 ATP (By similarity).
FT BINDING 308 308 ATP; via carbonyl oxygen (By similarity).
SQ SEQUENCE 498 AA; 54795 MW; B356F6A4418B51D3 CRC64;
MSGYILAIDQ GTTSTRSMLF DRNMRVVGLG QQEFTQHFPS SGWVEHDAEE IWKSVQSTIR
IALAQAGISA ADVAAIGITN QRETTVVWDR ISGKPVHRAI VWQDRRTAQF CDELKRRNLE
PLFTEKTGLL LDPYFSGTKL AWLLNHVPGL RERAQKGQVC FGTIDSWLIY KLTGGKAHVT
DATNASRTLI YHIGENRWDD ELLDILGIPA AMLPEVKDCA ADFGMTDPAL FGVSIPILGV
AGDQQTAVIG NACFEPGMMK STYGTGCFAL LNTGTDRVTS SNRLLTTIAY RLDGVTTYAL
EGSIFIAGAA VQWLRDEMGF ISVVSEVSAL AEKADPNQRI YLVPAFTGLG APYWDAEARG
AIFGLTRGTG RAEFARAALE SVAYQTFDLL EAMQGDWKGA TNHTVLRVDG GMVASDWTMQ
RLADILNAPV DRPVFLETTV LGAAWLAASR AGIWPDRKGF SERWQRDCRF EPAMPEKERE
SAIAGWRDSV SRCLTRPQ
//
