ID Q8IQ30_DROME Unreviewed; 1155 AA.
AC Q8IQ30;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 3.
DT 27-MAR-2024, entry version 154.
DE SubName: Full=Trabuco, isoform C {ECO:0000313|EMBL:AAN09550.3};
DE SubName: Full=Trabuco, isoform D {ECO:0000313|EMBL:AHN59964.1};
GN Name=tbc {ECO:0000313|EMBL:AAN09550.3,
GN ECO:0000313|FlyBase:FBgn0052506};
GN Synonyms=Dmel\CG32506 {ECO:0000313|EMBL:AAN09550.3};
GN ORFNames=CG32506 {ECO:0000313|EMBL:AAN09550.3,
GN ECO:0000313|FlyBase:FBgn0052506}, Dmel_CG32506
GN {ECO:0000313|EMBL:AAN09550.3};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [3] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [5] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [6] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [7] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S.,
RA Svirskas R., Rubin G.;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [9] {ECO:0000313|EMBL:AAN09550.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
RN [10] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109357; DOI=.1534/g3.115.018929;
RG FlyBase Consortium;
RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E.,
RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M.,
RA Gelbart W.M., null;
RT "Gene Model Annotations for Drosophila melanogaster: Impact of High-
RT Throughput Data.";
RL G3 (Bethesda) 5:1721-1736(2015).
RN [11] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109356; DOI=.1534/g3.115.018937;
RG FlyBase Consortium;
RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E.,
RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M.,
RA null;
RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.";
RL G3 (Bethesda) 5:1737-1749(2015).
RN [12] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25589440;
RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E.,
RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M.,
RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M.,
RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M.,
RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M.,
RA Karpen G.H., Celniker S.E.;
RT "The Release 6 reference sequence of the Drosophila melanogaster genome.";
RL Genome Res. 25:445-458(2015).
RN [13] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RG Berkeley Drosophila Genome Project;
RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA Rubin G.;
RT "Drosophila melanogaster release 4 sequence.";
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
RN [14] {ECO:0000313|EMBL:AAN09550.3}
RP NUCLEOTIDE SEQUENCE.
RG FlyBase;
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the RUTBC family.
CC {ECO:0000256|ARBA:ARBA00034124}.
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DR EMBL; AE014298; AAN09550.3; -; Genomic_DNA.
DR EMBL; AE014298; AHN59964.1; -; Genomic_DNA.
DR RefSeq; NP_001285494.1; NM_001298565.1.
DR RefSeq; NP_608395.6; NM_134551.7.
DR AlphaFoldDB; Q8IQ30; -.
DR SMR; Q8IQ30; -.
DR IntAct; Q8IQ30; 1.
DR STRING; 7227.FBpp0309347; -.
DR PaxDb; 7227-FBpp0300194; -.
DR EnsemblMetazoa; FBtr0307539; FBpp0300194; FBgn0052506.
DR EnsemblMetazoa; FBtr0340401; FBpp0309347; FBgn0052506.
DR GeneID; 318059; -.
DR KEGG; dme:Dmel_CG32506; -.
DR UCSC; CG32506-RB; d. melanogaster.
DR AGR; FB:FBgn0052506; -.
DR CTD; 318059; -.
DR FlyBase; FBgn0052506; tbc.
DR VEuPathDB; VectorBase:FBgn0052506; -.
DR eggNOG; KOG1648; Eukaryota.
DR GeneTree; ENSGT00940000168017; -.
DR HOGENOM; CLU_006235_0_0_1; -.
DR InParanoid; Q8IQ30; -.
DR OMA; LWPKSMR; -.
DR OrthoDB; 164430at2759; -.
DR BioGRID-ORCS; 318059; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 318059; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0052506; Expressed in brain and 11 other cell types or tissues.
DR ExpressionAtlas; Q8IQ30; baseline and differential.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProt.
DR GO; GO:0070971; C:endoplasmic reticulum exit site; IDA:FlyBase.
DR GO; GO:0005096; F:GTPase activator activity; ISS:FlyBase.
DR GO; GO:0009306; P:protein secretion; IGI:FlyBase.
DR GO; GO:0043087; P:regulation of GTPase activity; ISS:FlyBase.
DR CDD; cd15784; PH_RUTBC; 1.
DR CDD; cd17687; RUN_SGSM1_like; 1.
DR Gene3D; 1.20.58.900; -; 1.
DR Gene3D; 2.30.29.230; -; 1.
DR Gene3D; 1.10.8.270; putative rabgap domain of human tbc1 domain family member 14 like domains; 1.
DR Gene3D; 1.10.472.80; Ypt/Rab-GAP domain of gyp1p, domain 3; 1.
DR InterPro; IPR000195; Rab-GAP-TBC_dom.
DR InterPro; IPR035969; Rab-GAP_TBC_sf.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR InterPro; IPR037745; SGSM1/2.
DR InterPro; IPR021935; SGSM1/2_RBD.
DR PANTHER; PTHR22957; TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN; 1.
DR PANTHER; PTHR22957:SF696; TRABUCO, ISOFORM C; 1.
DR Pfam; PF12068; PH_RBD; 1.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR Pfam; PF02759; RUN; 1.
DR SMART; SM00593; RUN; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF140741; RUN domain-like; 1.
DR SUPFAM; SSF47923; Ypt/Rab-GAP domain of gyp1p; 2.
DR PROSITE; PS50826; RUN; 1.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000000803}.
FT DOMAIN 37..219
FT /note="RUN"
FT /evidence="ECO:0000259|PROSITE:PS50826"
FT DOMAIN 617..1088
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000259|PROSITE:PS50086"
FT REGION 95..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 716..838
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 884..910
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 98..130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 724..741
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 774..804
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 805..838
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1155 AA; 129414 MW; 2D44098E63070FE4 CRC64;
MVQGNADGSD YKERLIASVK KEVKQLMEEA VTKKYVHEES SSVTSLCGAV EACLSQGLRR
RALGLFKTSS TTALLHKIAK SCPEAEHISK LVQEIEASDP SKRSSSSSDS FQRPPMLKKS
SSNSMSTGTN AAASTSSASA SISVSASTSS MSLASMKYLW IRLALYEKRL TKIIEYLVSN
ASSFYDRDSL VADSDYGSIL SSLLVGPCAL EFTRAKTADH YWTDPHADEL VQRHRISSCR
RSSSTCSRPA IINFKRSLNT SSDEAGTGSF KSIASASVAK DYVESLHQNA KATLLYGKNN
VQVLPKDVAE PMPGYLSLHQ HIQTLTIKWT PNQLMNGYTE AEEAEDIDKD AFWAYALNIN
VDEIVYVHCH QSRGEDSGGT VILVGQDGVQ RPPIHFPEGG HMQQFLSCLE TGLLPHGQLD
PPLWSQRGIG KMFLWPRSMR RRILPSVMES VDETPIDYVF RIVSKSRHEE FAATHSILDF
VRSTPRRAQL SSCSTTGSSD CSNKSLSIDQ FPMESPLILQ QQQQQQQQLL QAQSTSIEMV
CSTMRRQIIS RAFYGWLAYC RHLSTVRTHL SGLVHGRITP EMKADEEGLT KERWQLLNVN
GVLENATEFY RLVYFGGVQP ELRQEVWPYL LGHYAFGSTT EDRKKQDETC KHYYETTMSE
WLAVDAIVQQ REKEKTARAV AKLSSGSNSG NDRTVRAADL EAGGDLENEV FEDISDISDP
GDLEFDEQQQ QQQQQAECAV SGNHLTVKPI PRAMKTSTDS GHVDESFNEL DEPDEEDNKQ
KQQQDEKISE SESKLPDYRK LEEQINQEPS CSASTASSYE TVGPGEVHQR PNSDTRSVLS
PEYLSADDLQ LPDDEDAVRP PPPPAAAAVI ITKASVDITN WERSPKAAEG DQMSPLEEQA
GESGGAGVNM DALQQPKSAC ASPASSNGGV YSSELLEQFG LNLHRIEKDV QRCDRNYWYF
ANENLDKLRN VISTYVWEHL DVGYMQGMCD LVAPLLVIFD DESLSYSCFC KLMERMIENF
PSGGAMDMHF ANMRSLIQIL DSEMYDLMDS NGDYTHFYFC YRWFLLDFKR ELVYDDVFAT
WEVIWAAKHI ASGHFVLFLA LALLETYRDI ILSNSMDFTD VIKFFNEMAE RHNAQSVLQL
ARSLVLQLQM IIENK
//
