ID ASSY_CHLTE Reviewed; 401 AA.
AC Q8KDE0;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 01-MAY-2013, entry version 71.
DE RecName: Full=Argininosuccinate synthase;
DE EC=6.3.4.5;
DE AltName: Full=Citrulline--aspartate ligase;
GN Name=argG; OrderedLocusNames=CT1114;
OS Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS).
OC Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC Chlorobaculum.
OX NCBI_TaxID=194439;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49652 / DSM 12025 / TLS;
RX PubMed=12093901; DOI=10.1073/pnas.132181499;
RA Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M.,
RA Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H.,
RA Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F.,
RA Holt I.E., Umayam L.A., Mason T.M., Brenner M., Shea T.P.,
RA Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B.,
RA Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M.,
RA Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M.;
RT "The complete genome sequence of Chlorobium tepidum TLS, a
RT photosynthetic, anaerobic, green-sulfur bacterium.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002).
CC -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC diphosphate + N(omega)-(L-arginino)succinate.
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC 1 subfamily.
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DR EMBL; AE006470; AAM72347.1; -; Genomic_DNA.
DR RefSeq; NP_662005.1; NC_002932.3.
DR ProteinModelPortal; Q8KDE0; -.
DR STRING; 194439.CT1114; -.
DR EnsemblBacteria; AAM72347; AAM72347; CT1114.
DR GeneID; 1006894; -.
DR KEGG; cte:CT1114; -.
DR PATRIC; 21400201; VBIChlTep116050_1012.
DR eggNOG; COG0137; -.
DR HOGENOM; HOG000230093; -.
DR KO; K01940; -.
DR OMA; NDQFRFE; -.
DR ProtClustDB; PRK00509; -.
DR BioCyc; CTEP194439:GHN0-1114-MONOMER; -.
DR UniPathway; UPA00068; UER00113.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.1260.10; -; 1.
DR HAMAP; MF_00005; Arg_succ_synth_type1; 1; -.
DR InterPro; IPR001518; Arginosuc_synth.
DR InterPro; IPR018223; Arginosuc_synth_CS.
DR InterPro; IPR023434; Arginosuc_synth_type_1_subfam.
DR InterPro; IPR024074; AS_cat/multimer_dom_body.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR11587; PTHR11587; 1.
DR Pfam; PF00764; Arginosuc_synth; 1.
DR TIGRFAMs; TIGR00032; argG; 1.
DR PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW Complete proteome; Cytoplasm; Ligase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1 401 Argininosuccinate synthase.
FT /FTId=PRO_0000148583.
FT NP_BIND 9 17 ATP (By similarity).
FT BINDING 88 88 Citrulline (By similarity).
FT BINDING 118 118 ATP; via amide nitrogen (By similarity).
FT BINDING 120 120 Aspartate (By similarity).
FT BINDING 124 124 Aspartate (By similarity).
FT BINDING 124 124 Citrulline (By similarity).
FT BINDING 125 125 Aspartate (By similarity).
FT BINDING 128 128 Citrulline (By similarity).
FT BINDING 177 177 Citrulline (By similarity).
FT BINDING 186 186 Citrulline (By similarity).
FT BINDING 262 262 Citrulline (By similarity).
FT BINDING 274 274 Citrulline (By similarity).
SQ SEQUENCE 401 AA; 44423 MW; 4ECE7F2DEC336A6D CRC64;
MSKEKIAVAY SGGLDTSVMI KWLKDKYEGA EIVAVTGNLG QKMEVDNLEQ KAIATGAKSF
HFVDLRKTFV EEYIWKALKA GALYEDVYPL ATALGRPLLA KALVDVALAE GCTMLTHGCT
GKGNDQVRFE VAFAALAPHM KVVAPLREWE FTSREQEIAY AMEHNIPVSA TKKNPYSIDE
NIWGISIECG VLEDPMVAPP ADAYQITTAP ELAPDEPTVV DIEFAQGVPV ALDGQQMEGL
DLIVRLNELG AMNGVGRLDM IENRVVGIKS REIYEAPAAT ILHFAHRELE RLTLEKSVFQ
YKRNIGQDYA NLIYNGTWFS PMRKALDAFV DETQKPVTGM VRIKLYKGSM TLLGRTSPNS
LYNEALATYT EADTFDHKSA EGFIKIYGLG LKTFHEVNKS E
//
