ID Q8KEJ2_CHLTE Unreviewed; 355 AA.
AC Q8KEJ2;
DT 01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2002, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE SubName: Full=ThiH protein {ECO:0000313|EMBL:AAM71934.1};
GN Name=thiH {ECO:0000313|EMBL:AAM71934.1};
GN OrderedLocusNames=CT0697 {ECO:0000313|EMBL:AAM71934.1};
OS Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
OS (Chlorobium tepidum).
OC Bacteria; Chlorobiota; Chlorobiia; Chlorobiales; Chlorobiaceae;
OC Chlorobaculum.
OX NCBI_TaxID=194439 {ECO:0000313|EMBL:AAM71934.1, ECO:0000313|Proteomes:UP000001007};
RN [1] {ECO:0000313|EMBL:AAM71934.1, ECO:0000313|Proteomes:UP000001007}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
RC {ECO:0000313|Proteomes:UP000001007};
RX PubMed=12093901; DOI=10.1073/pnas.132181499;
RA Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J.,
RA Deboy R., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D.,
RA Durkin A.S., Kolonay J.L., Yang F., Holt I., Umayam L.A., Mason T.,
RA Brenner M., Shea T.P., Parksey D., Nierman W.C., Feldblyum T.V.,
RA Hansen C.L., Craven M.B., Radune D., Vamathevan J., Khouri H., White O.,
RA Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A.,
RA Fraser C.M.;
RT "The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic,
RT anaerobic, green-sulfur bacterium.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; AE006470; AAM71934.1; -; Genomic_DNA.
DR RefSeq; NP_661592.1; NC_002932.3.
DR RefSeq; WP_010932379.1; NC_002932.3.
DR AlphaFoldDB; Q8KEJ2; -.
DR STRING; 194439.CT0697; -.
DR EnsemblBacteria; AAM71934; AAM71934; CT0697.
DR KEGG; cte:CT0697; -.
DR PATRIC; fig|194439.7.peg.640; -.
DR eggNOG; COG0502; Bacteria.
DR HOGENOM; CLU_046249_1_0_10; -.
DR OrthoDB; 9801120at2; -.
DR Proteomes; UP000001007; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR012726; ThiH.
DR InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR NCBIfam; TIGR02351; thiH; 1.
DR PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR PANTHER; PTHR43583:SF1; 2-IMINOACETATE SYNTHASE; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00876; BATS; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001007};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 46..266
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 355 AA; 39590 MW; E17EAB35D0F7EABC CRC64;
MIALPAWLTD ERLSEDIEPL LRQTDNESLE RLAAEAQAVT LRRFGRVISL YTPLYLSNFC
SSGCVYCGFA SDRRSPRRKL DTDEIEKELL AMKALGVSDV LLLTGERTNS VGFDYLRRAV
DIAARHMPRV AVEAFPMSVA EYRGLAECGC TGLTIYQETY DPDHYRELHR WGPKQDFLER
LETPERAITG GIRSVGIGAL LGLSEPVGEA LAVLRHARYL CKTYWKAGVT VSFPRIRPQE
GGFQPSFTVS DRFLARMIFA FRIGMPDVDL VLSTRESSNF RDGMAGLGIT RMSIASRTTV
GGYVEKETAG ASQFEVSDNR SVEAFCAALR AKDLEPVFKN WDAAYNNPLP AEECT
//
