ID Q8KHV8_ECOLX Unreviewed; 390 AA.
AC Q8KHV8;
DT 01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2002, sequence version 1.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=DNA gyrase subunit B {ECO:0000256|ARBA:ARBA00019166};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:BAC06612.1};
OS Escherichia coli.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:BAC06612.1};
RN [1] {ECO:0000313|EMBL:BAC06612.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC11775 {ECO:0000313|EMBL:BAC06612.1}, ATCC25922
RC {ECO:0000313|EMBL:BAC06744.1}, ECO15P2 {ECO:0000313|EMBL:BAC06647.1},
RC ECO18P1 {ECO:0000313|EMBL:BAC06693.1}, ECO18P10
RC {ECO:0000313|EMBL:BAC06698.1}, ECO18P11 {ECO:0000313|EMBL:BAC06706.1},
RC ECO18P12 {ECO:0000313|EMBL:BAC06699.1}, ECO18P13
RC {ECO:0000313|EMBL:BAC06700.1}, ECO18P14 {ECO:0000313|EMBL:BAC06707.1},
RC ECO18P15 {ECO:0000313|EMBL:BAC06708.1}, ECO18P16
RC {ECO:0000313|EMBL:BAC06701.1}, ECO18P2 {ECO:0000313|EMBL:BAC06694.1},
RC ECO18P3 {ECO:0000313|EMBL:BAC06695.1}, ECO18P5
RC {ECO:0000313|EMBL:BAC06703.1}, ECO18P6 {ECO:0000313|EMBL:BAC06704.1},
RC ECO18P7 {ECO:0000313|EMBL:BAC06705.1}, ECO18P8
RC {ECO:0000313|EMBL:BAC06696.1}, ECO18P9 {ECO:0000313|EMBL:BAC06697.1},
RC ECO1P10 {ECO:0000313|EMBL:BAC06619.1}, ECO1P3
RC {ECO:0000313|EMBL:BAC06615.1}, ECO1P4 {ECO:0000313|EMBL:BAC06616.1},
RC ECO1P7 {ECO:0000313|EMBL:BAC06617.1}, ECO1P9
RC {ECO:0000313|EMBL:BAC06618.1}, ECO25P3 {ECO:0000313|EMBL:BAC06714.1},
RC ECO25P5 {ECO:0000313|EMBL:BAC06715.1}, ECO26P3
RC {ECO:0000313|EMBL:BAC06718.1}, ECO55P2 {ECO:0000313|EMBL:BAC06723.1},
RC ECO6P1 {ECO:0000313|EMBL:BAC06725.1}, ECO6P3
RC {ECO:0000313|EMBL:BAC06726.1}, ECO6P4 {ECO:0000313|EMBL:BAC06727.1},
RC ECO6P5 {ECO:0000313|EMBL:BAC06728.1}, ECO6P6
RC {ECO:0000313|EMBL:BAC06729.1}, ECO6P7 {ECO:0000313|EMBL:BAC06730.1},
RC ECO6P8 {ECO:0000313|EMBL:BAC06733.1}, ECO6P9
RC {ECO:0000313|EMBL:BAC06731.1}, and ECO8P1
RC {ECO:0000313|EMBL:BAC06736.1};
RX PubMed=12149329; DOI=10.1128/JCM.40.8.2779-2785.2002;
RA Fukushima M., Kakinuma K., Kawaguchi R.;
RT "Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli
RT strains on the basis of the gyrB gene sequence.";
RL J. Clin. Microbiol. 40:2779-2785(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; AB083821; BAC06612.1; -; Genomic_DNA.
DR EMBL; AB083824; BAC06615.1; -; Genomic_DNA.
DR EMBL; AB083825; BAC06616.1; -; Genomic_DNA.
DR EMBL; AB083826; BAC06617.1; -; Genomic_DNA.
DR EMBL; AB083827; BAC06618.1; -; Genomic_DNA.
DR EMBL; AB083828; BAC06619.1; -; Genomic_DNA.
DR EMBL; AB083856; BAC06647.1; -; Genomic_DNA.
DR EMBL; AB083902; BAC06693.1; -; Genomic_DNA.
DR EMBL; AB083903; BAC06694.1; -; Genomic_DNA.
DR EMBL; AB083904; BAC06695.1; -; Genomic_DNA.
DR EMBL; AB083905; BAC06696.1; -; Genomic_DNA.
DR EMBL; AB083906; BAC06697.1; -; Genomic_DNA.
DR EMBL; AB083907; BAC06698.1; -; Genomic_DNA.
DR EMBL; AB083908; BAC06699.1; -; Genomic_DNA.
DR EMBL; AB083909; BAC06700.1; -; Genomic_DNA.
DR EMBL; AB083910; BAC06701.1; -; Genomic_DNA.
DR EMBL; AB083912; BAC06703.1; -; Genomic_DNA.
DR EMBL; AB083913; BAC06704.1; -; Genomic_DNA.
DR EMBL; AB083914; BAC06705.1; -; Genomic_DNA.
DR EMBL; AB083915; BAC06706.1; -; Genomic_DNA.
DR EMBL; AB083916; BAC06707.1; -; Genomic_DNA.
DR EMBL; AB083917; BAC06708.1; -; Genomic_DNA.
DR EMBL; AB083923; BAC06714.1; -; Genomic_DNA.
DR EMBL; AB083924; BAC06715.1; -; Genomic_DNA.
DR EMBL; AB083927; BAC06718.1; -; Genomic_DNA.
DR EMBL; AB083932; BAC06723.1; -; Genomic_DNA.
DR EMBL; AB083934; BAC06725.1; -; Genomic_DNA.
DR EMBL; AB083935; BAC06726.1; -; Genomic_DNA.
DR EMBL; AB083936; BAC06727.1; -; Genomic_DNA.
DR EMBL; AB083937; BAC06728.1; -; Genomic_DNA.
DR EMBL; AB083938; BAC06729.1; -; Genomic_DNA.
DR EMBL; AB083939; BAC06730.1; -; Genomic_DNA.
DR EMBL; AB083940; BAC06731.1; -; Genomic_DNA.
DR EMBL; AB083942; BAC06733.1; -; Genomic_DNA.
DR EMBL; AB083945; BAC06736.1; -; Genomic_DNA.
DR EMBL; AB083953; BAC06744.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8KHV8; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 313..390
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:BAC06612.1"
FT NON_TER 390
FT /evidence="ECO:0000313|EMBL:BAC06612.1"
SQ SEQUENCE 390 AA; 43291 MW; 5B2E034BF0FF3AEB CRC64;
DNSYKVSGGL HGVGVSVVNA LSQKLELVIQ REGKIHRQIY EHGVPQAPLA VTGETEKTGT
MVRFWPSLET FTNVTEFEYD ILAKRLRELS FLNSGVSIRL RDKRDGKEDH FHYEGGIKAF
VEYLNKNKTP IHPNIFYFST EKDGIGVEVA LQWNDGFQEN IYCFTNNIPQ RDGGTHLAGF
RAAMTRTLNA YMDKEGYSKK AKVSATGDDA REGLIAVVSV KVPDPKFSSQ TKDKLVSSEV
KSAVEQQMNE LLAEYLLENP TDAKIVVGKI IDAARAREAA RRAREMTRRK GALDLAGLPG
KLADCQERDP ALSELYLVEG DSAGGSAKQG RNRKNQAILP LKGKILNVEK ARFDKMLSSQ
EVATLITALG CGIGRDEYNP DKLRYHSIII
//
