ID Q8LK21_CHLRE Unreviewed; 752 AA.
AC Q8LK21;
DT 01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 2.
DT 27-MAR-2024, entry version 120.
DE SubName: Full=RNA polymerase sigma factor {ECO:0000313|EMBL:AAM88387.2};
DE SubName: Full=Sigma-like transcription factor {ECO:0000313|EMBL:CAF25319.1};
GN Name=RpoD {ECO:0000313|EMBL:AAM88387.2};
GN Synonyms=sig1 {ECO:0000313|EMBL:CAF25319.1};
GN ORFNames=CHLRE_03g194950v5 {ECO:0000313|EMBL:PNW85616.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000313|EMBL:AAM88387.2};
RN [1] {ECO:0000313|EMBL:AAM88387.2}
RP NUCLEOTIDE SEQUENCE.
RA Smith A.C., Purton S.;
RT "Nuclear-encoded gene for the chloroplast RNA polymerase sigma factor of
RT Chlamydomonas reinhardtii.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CAF25319.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CC125 {ECO:0000313|EMBL:CAF25319.1};
RA Bohne A.V.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF25319.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CC125 {ECO:0000313|EMBL:CAF25319.1};
RX PubMed=16453112; DOI=10.1007/s00294-006-0060-7;
RA Bohne A.V., Irihimovitch V., Weihe A., Stern D.B.;
RT "Chlamydomonas reinhardtii encodes a single sigma70-like factor which
RT likely functions in chloroplast transcription.";
RL Curr. Genet. 49:333-340(2006).
RN [4] {ECO:0000313|EMBL:PNW85616.1, ECO:0000313|Proteomes:UP000006906}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503 {ECO:0000313|Proteomes:UP000006906}, and CC-503 cw92 mt+
RC {ECO:0000313|EMBL:PNW85616.1};
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [5] {ECO:0000313|EMBL:PNW85616.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CC-503 cw92 mt+ {ECO:0000313|EMBL:PNW85616.1};
RG Chlamydomonas Annotation Team;
RG JGI Annotation Team;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Grigoriev I.V., Rokhsar D.S.;
RT "WGS assembly of Chlamydomonas reinhardtii.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family.
CC {ECO:0000256|ARBA:ARBA00007788}.
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DR EMBL; AF525691; AAM88387.2; -; Genomic_DNA.
DR EMBL; AJ627180; CAF25319.1; -; Genomic_DNA.
DR EMBL; CM008964; PNW85616.1; -; Genomic_DNA.
DR RefSeq; XP_001693275.1; XM_001693223.1.
DR STRING; 3055.Q8LK21; -.
DR PaxDb; 3055-EDP03301; -.
DR EnsemblPlants; PNW85616; PNW85616; CHLRE_03g194950v5.
DR GeneID; 5718733; -.
DR Gramene; PNW85616; PNW85616; CHLRE_03g194950v5.
DR KEGG; cre:CHLRE_03g194950v5; -.
DR eggNOG; ENOG502QVXR; Eukaryota.
DR HOGENOM; CLU_370225_0_0_1; -.
DR OMA; CEKTLHR; -.
DR OrthoDB; 33789at2759; -.
DR Proteomes; UP000006906; Chromosome 3.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:InterPro.
DR CDD; cd06171; Sigma70_r4; 1.
DR Gene3D; 1.10.601.10; RNA Polymerase Primary Sigma Factor; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; TIGR02937; sigma70-ECF; 1.
DR PANTHER; PTHR30603; RNA POLYMERASE SIGMA FACTOR RPO; 1.
DR PANTHER; PTHR30603:SF47; RNA POLYMERASE SIGMA FACTOR SIGF, CHLOROPLASTIC; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 2.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Reference proteome {ECO:0000313|Proteomes:UP000006906};
KW Sigma factor {ECO:0000256|ARBA:ARBA00023082};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015}.
FT DOMAIN 687..713
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00716"
FT REGION 110..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 280..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..651
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 304..319
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..334
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 752 AA; 80229 MW; AC90E5FE3A97384B CRC64;
MALPGSTMNL TTRCSTTPRS AVVARAVAAP TRPTTKSAVP ELLDSRPGER NLNFMEYAQA
TQMLDRLKGQ ASDLELLLDQ LNALEASLDE SVLAPPTVDD PKERAARQAR RAAKRAERRA
QATSATVAAA AGPAMSAVVS HSTPTKAAAA PATSTASSSS SDSGLLDLVS FVGGFDTRPI
PATTSAPPAG ASSSDVQHLE DLFKLSVGEP DIPRASASAA PAVLRPRKLT PKKPSAAPSA
AVTAAPSPAP TLPSTPSTSA RIAPAPGSLA DELERLLGPT TSREAAESED EDSFAGPSED
DLLALEQEVS RKSSRLPVLD EEDEEDEQQQ LEDNEEDAVA GPGSLEASAM ATRTSSQLSI
MQTGPSLLSL VPASAAPGRS AKARASRRAA RNGHASGRLG GATANAAGRG KVGSKDGTMN
FLGKVESLST LDVEKEREVT AVCRDFLFLE KVKRQCEKTL HRPATSEEIA AAVAMDVESL
KLRYDAGLKA KELLLKSNYK LVMTVCKSFV GKGPHIQDLV SEGVKGLLKG VEKYDATKGF
RFGTYAHWWI RQAVSRSLAE TGRAVRLPMH MIEQLTRLKN LSAKLQTQLA REPTLPELAK
AAGLPVTRVQ MLMETARSAA SLDTPIGGNE LGPTVKDSVE DEREAADEEF GSDSLRNDME
AMLLELPERE ARVVRLRFGL DDGKEWTLEE IGEALNVTRE RIRQIEAKAL RKLRVKTIDV
SGKLMEYGEN LEMLMDGSRE MAARTSSGTR KT
//
