ID Q8MUU2_GIAIN Unreviewed; 2076 AA.
AC Q8MUU2; A8BY59;
DT 01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2002, sequence version 1.
DT 24-JAN-2024, entry version 72.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=rpb1 {ECO:0000313|EMBL:AAM77743.1};
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741 {ECO:0000313|EMBL:AAM77743.1};
RN [1] {ECO:0000313|EMBL:AAM77743.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=12734189; DOI=10.1074/jbc.M303316200;
RA Seshadri V., McArthur A.G., Sogin M.L., Adam R.D.;
RT "Giardia lamblia RNA polymerase II: amanitin-resistant transcription.";
RL J. Biol. Chem. 278:27804-27810(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; AF510651; AAM77743.1; -; Genomic_DNA.
DR RefSeq; XP_001704218.1; XM_001704166.1.
DR GeneID; 5697088; -.
DR KEGG; gla:GL50803_0089347; -.
DR VEuPathDB; GiardiaDB:DHA2_89347; -.
DR VEuPathDB; GiardiaDB:GL50581_2856; -.
DR VEuPathDB; GiardiaDB:GL50803_0089347; -.
DR VEuPathDB; GiardiaDB:QR46_1940; -.
DR OrthoDB; 169836at2759; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0043229; C:intracellular organelle; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 2.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 234..552
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 2033..2076
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2033..2059
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2060..2076
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2076 AA; 230455 MW; A5B7F4467379C127 CRC64;
MDKEFALRIP YGVPKRVRFT LFSENEKNSL IPIVPRESSS DQMSMFSELN STRLGVIFQE
ENECPTCSGK AGDQDPSVLC PGHLGVVKFG YDIFHPMIID DLATLMNCFC PNCYHLKFFR
LFKETDRELA INALNRLRDT TPTLNFLPKL AELSATIACC ERNILYRKKA SGTSRNGVAP
QPLILAVSKS TRGSTARQAK DQDQLMDAHT CKLLITRYNK DDIVLLGLKR DHIENFIISS
IQVGSRAVRP SIRTDGTTAE DQLTEMYVSL FRNIRDCNNN SLNAGGGATG AKSQIGPNDI
YNAYKTLISA KGLNADGGSV PTKGIFERLS GKYGRMRSNL MGKRVNYCSR SVITGDPTIS
INELGVPRSV ASRLTFPEVV TARNRDFLMK LVSNGNRYPG AVSKTLPSGT VQLIDTQVIN
STGWGSSTDA DGRLGGETTN DPLPLGTIVN RHLLDGDYVL FNRQPTLHKS SFMGHRVKVL
PYSTYRLNLS ATSSYNADFD GDEMNLHALQ SLEAVAEISE LCMVSNQVVS VKDNRPIVYI
VQDVLLGCYL FTGKDVTIPF ARVCEYIMWM GFSRTSDPTY AHKLQRYDKG SVNQRGHSSA
SMSYDFSDHT SGSYNPAEYD SSSYLVGNSA TGSVVDGRGS YLSTGNYGAS SASRKAYEDN
INNDNGAYFK RPGSNVNTND HVLLNVSQPA ICYPEARWTG KQVFSMFVPR GVFYRSGDPK
DISAHADKYI SFLDGSVMCG RIASSVVGGT ESALIHILFR DYGIEPCRAF IDNCQRVVCR
FMLDHGFSVG MGDMVSSEHT ERKVAEIQTK LSKDISELFK LSIHYKIKLA PGQSRNDAFE
QEVISKVSGT SLALEKVITD AAPHRNALLV MINAGSKGKK FNMMQISSSL GQQFLQSKRM
PHRFETHRSL PCYSPFSSLN MESRGYIFNS FIRGLNPAEF YFHAVGGREG VCDTAVKTAD
SGYVERKLLK VMESVCYRYD NSVRNDSNEI ISFRYGDDGI DPHKSESREF KDFSISDMDF
LRAHYIDWSQ IVDFQNTEMD ESTDFYATLV REKFAVIIAK LRDTPEQFAH LCDGVSFYEV
CDDELRAKGF DNLVLDNNLV SFTELSSYLQ NHYALFMDAE SSPGYVPENI DDSWLDIGVK
SSLVLERDFA ISTILVEYIV LLLERIWLQR SRITLDTDVK KFTFGMNLDR IIQSEIQAGS
INKVSRLIRE SAWRCYLKPF EAIEKVNKLL SRIKSFRPKE STLFRVILRQ CLSSKSCCYK
YRLTSEQLDR ILATIEEKFV RGQVSPGEPV GPLAGHSVSE PATQLTLNTF HTAGTSALGG
LGLPRLKELI DFRNPKISVS TIYLNISNQD PANAGVDTRC TSHSPMLFNR PVAPRPNSYN
IASAEEATGR NIRQSKAWMN LAAKIEHTTF NYYIDDYKLI FDPFDELTCV ADDREWLHLE
SEIGHLQQQV YGERPNEAVR FYSPFVLRYE IGLKQMQQKI EAGITIQTLV SRLILMLQQI
YTSRAGRPKS EDDDEDDEDD INIKQKAMIG REMPIINYYT GLGKTVIRVR PSLTEDEYNA
VKGSAIGSGA PDMEKSRLLA ERYDGVDAFL RNVHISGNES IRKVFMASSN PLVHYGYDGF
TSNVDVFSQG SSDGLSMHKM SEGDLSKIGS IGKFTQPNSD ISELYLQTDG SDLLWILTQP
EVDFTRTWTT NVREIYEILG IEAARALFIK QIRATLDKVD LNIHHYLILA DIMSSRPQNN
KMISINRYGM QATNTGVLAQ ATFERPGATV LKAAAFGVVD PLKSVSSCVV MGKQGDFGTG
CFDLLLNCAE LPSINEDELF PNSYYLKVHN ATTEVRPTAG AEETPVDSMG MPFSTTFISP
TATAMVTPTS TYTSHSSVRS VVSAGGRVDA NMNPLFSPGC NRSRQAENAS RIFSSYTANT
LTSAKSSLPT QLTITSRSRG LSNIVDGRDS RIASITSAVN SKMSAFSMTS CNSINSFRSA
LASKASTHSN QYDTSDLVDV KGAGRYGIFT HSTNMSYENT AASIRNSGGM FASRSGSASS
VRSQSATNID ASAADSGQSG ELRDNHEDDN NDYAEW
//
