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Database: UniProt
Entry: Q8N3J2
LinkDB: Q8N3J2
Original site: Q8N3J2 
ID   METL4_HUMAN             Reviewed;         472 AA.
AC   Q8N3J2; B2RNA1; Q2TAA7; Q9H5U9;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 3.
DT   01-OCT-2014, entry version 90.
DE   RecName: Full=Methyltransferase-like protein 4;
DE            EC=2.1.1.-;
GN   Name=METTL4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16177791; DOI=10.1038/nature03983;
RA   Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D.,
RA   Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K.,
RA   FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S.,
RA   Bloom T., Bugalter B., Butler J., Cook A., DeCaprio D., Engels R.,
RA   Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T.,
RA   Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E.,
RA   Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H.,
RA   O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA   Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA   Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 18.";
RL   Nature 437:551-555(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS LYS-310 AND
RP   LEU-468.
RC   TISSUE=Brain, Lung, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 137-472, AND VARIANT
RP   LEU-468.
RC   TISSUE=Lung;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 228-472, AND VARIANT
RP   LEU-468.
RC   TISSUE=Melanoma;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA   Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA   Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [6]
RP   VARIANT PHE-42.
RX   PubMed=21248752; DOI=10.1038/nature09639;
RA   Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P.,
RA   Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A.,
RA   Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C.,
RA   Jia M., Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A.,
RA   Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S.,
RA   Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A.,
RA   Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C.,
RA   Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R.,
RA   Futreal P.A.;
RT   "Exome sequencing identifies frequent mutation of the SWI/SNF complex
RT   gene PBRM1 in renal carcinoma.";
RL   Nature 469:539-542(2011).
CC   -!- FUNCTION: Probable methyltransferase. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MT-A70-like family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00489}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB15520.1; Type=Frameshift; Positions=343; Evidence={ECO:0000305};
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DR   EMBL; AP005136; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471113; EAX01698.1; -; Genomic_DNA.
DR   EMBL; BC111020; AAI11021.1; -; mRNA.
DR   EMBL; BC136766; AAI36767.1; -; mRNA.
DR   EMBL; BC136767; AAI36768.1; -; mRNA.
DR   EMBL; AK026670; BAB15520.1; ALT_FRAME; mRNA.
DR   EMBL; AL834296; CAD38969.1; -; mRNA.
DR   CCDS; CCDS11826.1; -.
DR   RefSeq; NP_073751.3; NM_022840.3.
DR   UniGene; Hs.126888; -.
DR   ProteinModelPortal; Q8N3J2; -.
DR   BioGrid; 122334; 1.
DR   STRING; 9606.ENSP00000320349; -.
DR   PhosphoSite; Q8N3J2; -.
DR   DMDM; 269849669; -.
DR   PaxDb; Q8N3J2; -.
DR   PRIDE; Q8N3J2; -.
DR   Ensembl; ENST00000574538; ENSP00000458290; ENSG00000101574.
DR   GeneID; 64863; -.
DR   KEGG; hsa:64863; -.
DR   UCSC; uc002klh.4; human.
DR   CTD; 64863; -.
DR   GeneCards; GC18M002527; -.
DR   H-InvDB; HIX0014297; -.
DR   HGNC; HGNC:24726; METTL4.
DR   HPA; HPA040061; -.
DR   neXtProt; NX_Q8N3J2; -.
DR   PharmGKB; PA134916468; -.
DR   eggNOG; COG4725; -.
DR   HOGENOM; HOG000113569; -.
DR   HOVERGEN; HBG078120; -.
DR   InParanoid; Q8N3J2; -.
DR   OMA; LIVSVPC; -.
DR   PhylomeDB; Q8N3J2; -.
DR   TreeFam; TF314410; -.
DR   GenomeRNAi; 64863; -.
DR   NextBio; 67019; -.
DR   PRO; PR:Q8N3J2; -.
DR   ArrayExpress; Q8N3J2; -.
DR   Bgee; Q8N3J2; -.
DR   CleanEx; HS_METTL4; -.
DR   Genevestigator; Q8N3J2; -.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006139; P:nucleobase-containing compound metabolic process; IEA:InterPro.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR007757; MT-A70-like.
DR   InterPro; IPR029063; SAM-dependent_MTases-like.
DR   Pfam; PF05063; MT-A70; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51143; MT_A70; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Methyltransferase; Polymorphism;
KW   Reference proteome; Transferase.
FT   CHAIN         1    472       Methyltransferase-like protein 4.
FT                                /FTId=PRO_0000251225.
FT   VARIANT      42     42       S -> F (found in a renal cell carcinoma
FT                                sample; somatic mutation).
FT                                {ECO:0000269|PubMed:21248752}.
FT                                /FTId=VAR_064731.
FT   VARIANT     163    163       L -> F (in dbSNP:rs12606220).
FT                                /FTId=VAR_027670.
FT   VARIANT     230    230       E -> D (in dbSNP:rs34143130).
FT                                /FTId=VAR_056159.
FT   VARIANT     310    310       Q -> K (in dbSNP:rs2677879).
FT                                {ECO:0000269|PubMed:15489334}.
FT                                /FTId=VAR_027671.
FT   VARIANT     468    468       V -> L (in dbSNP:rs8084295).
FT                                {ECO:0000269|PubMed:14702039,
FT                                ECO:0000269|PubMed:15489334,
FT                                ECO:0000269|PubMed:17974005}.
FT                                /FTId=VAR_027672.
FT   CONFLICT    153    153       K -> M (in Ref. 4; BAB15520).
FT                                {ECO:0000305}.
SQ   SEQUENCE   472 AA;  54041 MW;  5A5D77484AF358D7 CRC64;
     MSVVHQLSAG WLLDHLSFIN KINYQLHQHH EPCCRKKEFT TSVHFESLQM DSVSSSGVCA
     AFIASDSSTK PENDDGGNYE MFTRKFVFRP ELFDVTKPYI TPAVHKECQQ SNEKEDLMNG
     VKKEISISII GKKRKRCVVF NQGELDAMEY HTKIRELILD GSLQLIQEGL KSGFLYPLFE
     KQDKGSKPIT LPLDACSLSE LCEMAKHLPS LNEMEHQTLQ LVEEDTSVTE QDLFLRVVEN
     NSSFTKVITL MGQKYLLPPK SSFLLSDISC MQPLLNYRKT FDVIVIDPPW QNKSVKRSNR
     YSYLSPLQIQ QIPIPKLAAP NCLLVTWVTN RQKHLRFIKE ELYPSWSVEV VAEWHWVKIT
     NSGEFVFPLD SPHKKPYEGL ILGRVQEKTA LPLRNADVNV LPIPDHKLIV SVPCTLHSHK
     PPLAEVLKDY IKPDGEYLEL FARNLQPGWT SWGNEVLKFQ HVDYFIAVES GS
//
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