ID CRTM_STAAW Reviewed; 287 AA.
AC Q8NUQ5;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 01-MAY-2013, entry version 64.
DE RecName: Full=Dehydrosqualene synthase;
DE EC=2.5.1.96;
DE AltName: Full=4,4'-diapophytoene synthase;
DE Short=DAP synthase;
DE AltName: Full=Diapophytoene synthase;
GN Name=crtM; OrderedLocusNames=MW2483;
OS Staphylococcus aureus (strain MW2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcus.
OX NCBI_TaxID=196620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MW2;
RX PubMed=12044378; DOI=10.1016/S0140-6736(02)08713-5;
RA Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A.,
RA Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L.,
RA Yamamoto K., Hiramatsu K.;
RT "Genome and virulence determinants of high virulence community-
RT acquired MRSA.";
RL Lancet 359:1819-1827(2002).
CC -!- FUNCTION: Catalyzes the head-to-head condensation of two molecules
CC of farnesyl diphosphate (FPP) into the colorless C(30) carotenoid
CC dehydrosqualene (4,4'-diapophytoene). This is the initial step in
CC the biosynthesis of staphyloxanthin, an orange carotenoid present
CC in most staphylococci strains (By similarity).
CC -!- CATALYTIC ACTIVITY: 2 (2E,6E)-farnesyl diphosphate = 4,4'-
CC diapophytoene + 2 diphosphate.
CC -!- PATHWAY: Carotenoid biosynthesis; staphyloxanthin biosynthesis;
CC staphyloxanthin from farnesyl diphosphate: step 1/5.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. CrtM
CC subfamily.
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DR EMBL; BA000033; BAB96348.1; -; Genomic_DNA.
DR RefSeq; NP_647300.1; NC_003923.1.
DR ProteinModelPortal; Q8NUQ5; -.
DR SMR; Q8NUQ5; 1-284.
DR STRING; 196620.MW2483; -.
DR EnsemblBacteria; BAB96348; BAB96348; BAB96348.
DR GeneID; 1004619; -.
DR KEGG; sam:MW2483; -.
DR PATRIC; 19571710; VBIStaAur44266_2566.
DR eggNOG; COG1562; -.
DR HOGENOM; HOG000220848; -.
DR KO; K10208; -.
DR OMA; ELKLYCY; -.
DR ProtClustDB; CLSK886072; -.
DR BioCyc; SAUR196620:GJ9Z-2559-MONOMER; -.
DR UniPathway; UPA00029; UER00556.
DR GO; GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IEA:InterPro.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR008949; Terpenoid_synth.
DR Pfam; PF00494; SQS_PSY; 1.
DR SUPFAM; SSF48576; Terpenoid_synth; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; FALSE_NEG.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; Complete proteome; Transferase.
FT CHAIN 1 287 Dehydrosqualene synthase.
FT /FTId=PRO_0000282623.
FT REGION 18 22 Farnesyl diphosphate 1 binding (By
FT similarity).
FT REGION 45 48 Farnesyl diphosphate 2 binding (By
FT similarity).
FT BINDING 41 41 Farnesyl diphosphate 1 (By similarity).
FT BINDING 168 168 Farnesyl diphosphate 2 (By similarity).
FT BINDING 171 171 Farnesyl diphosphate 1 (By similarity).
FT BINDING 172 172 Farnesyl diphosphate 2 (By similarity).
FT BINDING 248 248 Farnesyl diphosphate 1 (By similarity).
SQ SEQUENCE 287 AA; 34231 MW; 2407009413D42E63 CRC64;
MTMMDMNFKY CHKIMKKHSK SFSYAFDLLP EDQRKAVWAI YAVCRKIDDS IDVYGDIQFL
NQIKEDIQSI EKYPYEHHHF QSDRRIMMAL QHVAQHKNIA FQSFYNLIDT VYKDQHFTMF
ETDAELFGYC YGVAGTVGEV LTPILSDHET HQTYDVARRL GESLQLINIL RDVGEDFDNE
RIYFSKQRLK QYEVDIAEVY QNGVNNHYID LWEYYAAIAE KDFQDVMDQI KVFSIEAQPI
IELAARIYIE ILDEVRQANY TLHERVFVDK RKKAKLFHEI NSKYHRI
//
