ID Q8RJG6_BRAJP Unreviewed; 303 AA.
AC Q8RJG6;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:BAB85505.1};
OS Bradyrhizobium japonicum.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=375 {ECO:0000313|EMBL:BAB85505.1};
RN [1] {ECO:0000313|EMBL:BAB85505.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC10324 {ECO:0000313|EMBL:BAB85505.1}, and ATCC35281
RC {ECO:0000313|EMBL:BAB85506.1};
RA Sameshima R., Isawa T., Sadowsky M.J., Hamada T., Kasai H., Shutsrirung A.,
RA Mitsui H., Minamisawa K.;
RT "Phylogeny and distribution of extra-slow-growing Bradyrhizobium japonicum
RT harboring high copy numbers of RSalpha, RSbeta and IS1631.";
RL FEMS Microbiol. Ecol. 44:191-202(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; AB070586; BAB85505.1; -; Genomic_DNA.
DR EMBL; AB070587; BAB85506.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8RJG6; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 3..148
FT /note="Histidine kinase/HSP90-like ATPase"
FT /evidence="ECO:0000259|SMART:SM00387"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:BAB85505.1"
FT NON_TER 303
FT /evidence="ECO:0000313|EMBL:BAB85505.1"
SQ SEQUENCE 303 AA; 33008 MW; 131E8AA1BBE8F87D CRC64;
DTDDGSGLHH MVYEVVDNAI DEALAGHATR VDVVLNADNS VTVRDDGRGI PVDIHKGEGI
SAAEVIMTQL HAGGKFDQNS YKVSGGLHGV GVSVVNALSS KLGLRIWRDD KEHYIEFAHG
DAVAPLKVVG DAPGRRGTEV TFLASPETFK NIEYDFATLE HRLRELAFLN SGVNIILSDM
RHAVEKREEM HYSGGVEEFV KYLDRNKKAI VPAPIMVRAE ANGIGVEAAL WWNDSYHENV
LCFTNNIPQR DGGTHLAGFR GALTRQVNGY AEANAKKEKI ALTGDDCREG LTAVLSVKVP
DPK
//
