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Database: UniProt
Entry: Q8RXF1
LinkDB: Q8RXF1
Original site: Q8RXF1 
ID   SF3A1_ARATH             Reviewed;         785 AA.
AC   Q8RXF1; Q0WWB5; Q9MA20;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 2.
DT   26-NOV-2014, entry version 99.
DE   RecName: Full=Probable splicing factor 3A subunit 1;
GN   OrderedLocusNames=At1g14650; ORFNames=T5E21.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22223895; DOI=10.1074/mcp.M111.015131;
RA   Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C.,
RA   Meinnel T., Giglione C.;
RT   "Comparative large-scale characterisation of plant vs. mammal proteins
RT   reveals similar and idiosyncratic N-alpha acetylation features.";
RL   Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN   [6]
RP   STRUCTURE BY NMR OF 683-780.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of ubiquitin-like domain in splicing factor
RT   AAL91182.";
RL   Submitted (NOV-2004) to the PDB data bank.
CC   -!- SUBUNIT: Component of splicing factor SF3A which is composed of
CC       three subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: SURP motif 2 mediates direct binding to SF3A3.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Contains 2 SURP motif repeats. {ECO:0000255|PROSITE-
CC       ProRule:PRU00263}.
CC   -!- SIMILARITY: Contains 1 ubiquitin-like domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00214}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF63169.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC010657; AAF63169.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE29196.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE29197.1; -; Genomic_DNA.
DR   EMBL; AY081293; AAL91182.1; -; mRNA.
DR   EMBL; AK226440; BAE98583.1; -; mRNA.
DR   PIR; G86280; G86280.
DR   RefSeq; NP_001117289.1; NM_001123817.1.
DR   RefSeq; NP_172917.1; NM_101332.3.
DR   UniGene; At.22146; -.
DR   PDB; 1WE6; NMR; -; A=683-780.
DR   PDBsum; 1WE6; -.
DR   ProteinModelPortal; Q8RXF1; -.
DR   SMR; Q8RXF1; 67-121, 173-314, 683-782.
DR   IntAct; Q8RXF1; 1.
DR   PaxDb; Q8RXF1; -.
DR   PRIDE; Q8RXF1; -.
DR   EnsemblPlants; AT1G14650.1; AT1G14650.1; AT1G14650.
DR   EnsemblPlants; AT1G14650.2; AT1G14650.2; AT1G14650.
DR   GeneID; 838027; -.
DR   KEGG; ath:AT1G14650; -.
DR   TAIR; AT1G14650; -.
DR   eggNOG; NOG300902; -.
DR   HOGENOM; HOG000238941; -.
DR   InParanoid; Q8RXF1; -.
DR   KO; K12825; -.
DR   OMA; MILENTA; -.
DR   PhylomeDB; Q8RXF1; -.
DR   Reactome; REACT_180054; mRNA Splicing - Major Pathway.
DR   EvolutionaryTrace; Q8RXF1; -.
DR   PRO; PR:Q8RXF1; -.
DR   ExpressionAtlas; Q8RXF1; baseline and differential.
DR   Genevestigator; Q8RXF1; -.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   InterPro; IPR022030; PRP21-like.
DR   InterPro; IPR000061; Surp.
DR   InterPro; IPR000626; Ubiquitin-like.
DR   InterPro; IPR029071; Ubiquitin-rel_dom.
DR   Pfam; PF12230; PRP21_like_P; 1.
DR   Pfam; PF01805; Surp; 2.
DR   Pfam; PF00240; ubiquitin; 1.
DR   SMART; SM00648; SWAP; 2.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF109905; SSF109905; 2.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50128; SURP; 2.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Complete proteome; mRNA processing;
KW   mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome.
FT   CHAIN         1    785       Probable splicing factor 3A subunit 1.
FT                                /FTId=PRO_0000114919.
FT   REPEAT       71    113       SURP motif 1.
FT   REPEAT      193    235       SURP motif 2.
FT   DOMAIN      707    782       Ubiquitin-like. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00214}.
FT   COMPBIAS    287    386       Glu-rich.
FT   COMPBIAS    536    677       Pro-rich.
FT   COMPBIAS    588    660       Met-rich.
FT   SITE        196    196       Critical for binding to SF3A3.
FT                                {ECO:0000250}.
FT   MOD_RES       1      1       N-acetylmethionine.
FT                                {ECO:0000269|PubMed:22223895}.
FT   CONFLICT    289    289       D -> G (in Ref. 3; AAL91182).
FT                                {ECO:0000305}.
FT   HELIX       686    688       {ECO:0000244|PDB:1WE6}.
FT   HELIX       692    698       {ECO:0000244|PDB:1WE6}.
FT   STRAND      703    707       {ECO:0000244|PDB:1WE6}.
FT   STRAND      713    715       {ECO:0000244|PDB:1WE6}.
FT   STRAND      718    723       {ECO:0000244|PDB:1WE6}.
FT   STRAND      725    728       {ECO:0000244|PDB:1WE6}.
FT   HELIX       729    739       {ECO:0000244|PDB:1WE6}.
FT   TURN        744    746       {ECO:0000244|PDB:1WE6}.
FT   STRAND      747    750       {ECO:0000244|PDB:1WE6}.
FT   STRAND      752    755       {ECO:0000244|PDB:1WE6}.
FT   TURN        762    766       {ECO:0000244|PDB:1WE6}.
FT   STRAND      768    770       {ECO:0000244|PDB:1WE6}.
FT   STRAND      772    776       {ECO:0000244|PDB:1WE6}.
SQ   SEQUENCE   785 AA;  87594 MW;  917E388B77472F8D CRC64;
     MFSSMQILPL EAPPTDGKLG PLPPSQLTDQ EVEERELQAE QNNSNLAPPA AVATHTRTIG
     IIHPPPDIRT IVEKTAQFVS KNGLEFEKRI IVSNEKNAKF NFLKSSDPYH AFYQHKLTEY
     RAQNKDGAQG TDDSDGTTDP QLDTGAADES EAGDTQPDLQ AQFRIPSKPL EAPEPEKYTV
     RLPEGITGEE LDIIKLTAQF VARNGKSFLT GLSNRENNNP QFHFMKPTHS MFTFFTSLVD
     AYSEVLMPPK DLKEKLRKSA ADLTTVLERC LHRLEWDRSQ EQQKKKEEDE KELERVQMAM
     IDWHDFVVVE SIDFADEEDE ELPPPMTLDE VIRRSKASAM EEDEIVEPGK EVEMEMDEEE
     VKLVAEGMRA ANLEENVKIE NVHDEEAPMR IVKNWKRPED RIPTERDPTK VVISPITGEL
     IPINEMSEHM RISLIDPKFK EQKDRMFAKI RETTLAQDDE IAKNIVGLAR LRPDIFGTTE
     EEVSNAVKAE IEKKKDEQPK QVIWDGHTGS IGRTANQALS QNANGEEQGD GVYGDPNSFP
     GPAALPPPRP GVPIVRPLPP PPNLALNLPR PPPSAQYPGA PRPLGVPMMQ PMHQQHQLTM
     PGPPGHPQMM MNRPPQMQPG MHVPPPPGSQ FAHHMQIPRP YGQLPPSAMG MMQPPPMPGM
     APPPPPEEAP PPLPEEPEAK RQKFDESALV PEDQFLAQHP GPATIRVSKP NENDGQFMEI
     TVQSLSENVG SLKEKIAGEI QIPANKQKLS GKAGFLKDNM SLAHYNVGAG EILTLSLRER
     GGRKR
//
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