ID QKGA_DICDI Reviewed; 1553 AA.
AC Q8SSS9; Q557D4;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 01-MAY-2013, entry version 85.
DE RecName: Full=Probable serine/threonine-protein kinase qkgA;
DE EC=2.7.11.1;
DE AltName: Full=Quick growth protein qkgA;
DE AltName: Full=Ras of complex proteins and C-terminal of roc 2;
GN Name=qkgA-1; Synonyms=roco2-1; ORFNames=DDB_G0273259;
GN and
GN Name=qkgA-2; Synonyms=roco2-2; ORFNames=DDB_G0273635;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14654223; DOI=10.1016/j.bbamcr.2003.08.008;
RA Bosgraaf L., van Haastert P.J.M.;
RT "Roc, a Ras/GTPase domain in complex proteins.";
RL Biochim. Biophys. Acta 1643:5-10(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A.,
RA Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G.,
RA Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A.,
RA Platzer M., Rosenthal A., Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP FUNCTION.
RX PubMed=12954783; DOI=10.1093/nar/gng095;
RA Abe T., Langenick J., Williams J.G.;
RT "Rapid generation of gene disruption constructs by in vitro
RT transposition and identification of a Dictyostelium protein kinase
RT that regulates its rate of growth and development.";
RL Nucleic Acids Res. 31:E107-E107(2003).
CC -!- FUNCTION: Involved in growth, and during development, in
CC aggregation.
CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
CC protein kinase family. ROCO subfamily.
CC -!- SIMILARITY: Contains 4 LRR (leucine-rich) repeats.
CC -!- SIMILARITY: Contains 1 protein kinase domain.
CC -!- SIMILARITY: Contains 1 Roc domain.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3
CC and AX4. These strains contain a duplication of a segment of 750
CC kb of chromosome 2 compared to the corresponding sequence in
CC strain AX2.
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DR EMBL; AY232265; AAO83648.1; -; Genomic_DNA.
DR EMBL; AAFI02000011; EAL70508.1; -; Genomic_DNA.
DR EMBL; AAFI02000009; EAL70847.1; -; Genomic_DNA.
DR RefSeq; XP_644434.1; XM_639342.1.
DR RefSeq; XP_644816.1; XM_639724.1.
DR HSSP; O43318; 2EVA.
DR ProteinModelPortal; Q8SSS9; -.
DR STRING; 44689.DDB_0185215; -.
DR PRIDE; Q8SSS9; -.
DR EnsemblProtists; DDB0185215; DDB0185215; DDB_G0273259.
DR EnsemblProtists; DDB0304847; DDB0304847; DDB_G0273635.
DR GeneID; 8618918; -.
DR GeneID; 8619059; -.
DR KEGG; ddi:DDB_G0273259; -.
DR KEGG; ddi:DDB_G0273635; -.
DR dictyBase; DDB_G0273259; qkgA-1.
DR dictyBase; DDB_G0273635; qkgA-2.
DR eggNOG; COG1100; -.
DR OMA; CAIVENG; -.
DR ProtClustDB; CLSZ2431126; -.
DR GO; GO:0005622; C:intracellular; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0031158; P:negative regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0008285; P:negative regulation of cell proliferation; IMP:dictyBase.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IMP:dictyBase.
DR InterPro; IPR011009; Kinase-like_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR013684; MIRO-like.
DR InterPro; IPR000719; Prot_kinase_cat_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR020859; ROC_GTPase.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF08477; Miro; 1.
DR Pfam; PF07714; Pkinase_Tyr; 1.
DR SUPFAM; SSF56112; Kinase_like; 1.
DR PROSITE; PS51450; LRR; 3.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; FALSE_NEG.
DR PROSITE; PS51424; ROC; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Kinase; Leucine-rich repeat;
KW Nucleotide-binding; Reference proteome; Repeat;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1 1553 Probable serine/threonine-protein kinase
FT qkgA.
FT /FTId=PRO_0000355208.
FT REPEAT 287 309 LRR 1.
FT REPEAT 311 333 LRR 2.
FT REPEAT 334 356 LRR 3.
FT REPEAT 357 378 LRR 4.
FT DOMAIN 395 619 Roc.
FT DOMAIN 1242 1546 Protein kinase.
FT NP_BIND 1248 1256 ATP (By similarity).
FT COMPBIAS 112 126 Poly-Ser.
FT COMPBIAS 648 659 Poly-Ser.
FT COMPBIAS 663 670 Poly-Ser.
FT COMPBIAS 684 699 Poly-Gln.
FT COMPBIAS 999 1004 Poly-Ser.
FT COMPBIAS 1008 1018 Poly-Ser.
FT COMPBIAS 1059 1081 Poly-Thr.
FT COMPBIAS 1086 1089 Poly-Asn.
FT COMPBIAS 1277 1295 Poly-Asn.
FT COMPBIAS 1298 1301 Poly-Asn.
FT ACT_SITE 1393 1393 Proton acceptor (By similarity).
FT BINDING 1271 1271 ATP (By similarity).
SQ SEQUENCE 1553 AA; 174817 MW; 734E5D777ADF1C0C CRC64;
MDLEQDEWMS QRQRQQQMEF SRIGIEVIGS KKDLIVNSMK NHETTLTIIN ETYRIVYYDQ
YESKFTSCNI ITFDGKDKRS IKKMKRVVQK LSNESTNSSH QFDLHSHALL QSSSSSSSSS
TSSSPSLTSS PSSPISTSPP YHSSPQLLEH LLHQQQPITS VGSKPSFFVI INLYNDDRQS
YMSEIVSFAG SFQFIEWRND ESWKEVFKLS SNLLTYHVKK NELARACTLG DINLLDEIIL
SGCSKSQLKE AQGAGYTIVF NSYTSYSSGS SLVVSGTMAL LCAIVENGTF LLLSKSNITR
FPMSIINMCS QLVELDMSNN RITEIPIEIT ELKFLKNLNL SDNLINDIPL EICNLTLLKV
LLLNENPLNN FPSSIVELGT KKLLSFCRNI LEGKSCETWN KVKLMFVGEE GVGKTRLCKL
LIGSKYNKKK SSSSSSSSLS SLSSALSSSS SSSSIINSNS ISNSNINMVN IKNENQVTGD
TVSTEGVNIH DFKSKKVEFY AWDFGGQQIF YPTHQFFLTT QSLYLLIFRV NDSNFAERVN
YWVHQIKAKS GISLPIIFFV GTHIDSCTPE QLSMAESILK SNFIKYSRVK QNVINFVSCV
TGKGVKELKK RLIHESEKSR LIKKDIPGSY IILEQRLSNR GANQGRMSIS NSASNSSSSL
LNSSSSSSPS LTSKKSSSHL KHSQQQQQQH QQQQQQLQQS IKEKYIDYDD YLNECKLSYL
KPHEIKDATD FLHNLGIILH FDSPKLNNLV VLDPQWLADV MSSLITFSHS WIKLGILNHS
DLQSIWGGRY KQTLWPSLLK LLEKFEVSYQ LPNLSKSLIP SLLPDDPIGE ILEIKDREWV
PLKLAMENNY VQVFGCDYHF SFMPLGFFAR LLLRILLIQG IDIKTYWKDG VLLDILTPSD
KIKQQQLLQK KSIFEPTTTL SPFLCSLSLT DSQNYSPLEP PPLILNSPRV NRKFISNSTS
TTSTTTSTTS TSTTSSNNIT PLSMSSITPY SPTSPRTPSF SSSSTTTSSS SSSSSPSSQF
CGVGCHRNIN KNNGSSNELI KTLTKINNQN GSSGELIKTT TTTSTSTSTT STTTPTTTTP
TIPIKNNNNK KLPINRTVSS LCLTAKPKLQ ALITFIKKKS FEQSHDNDSY KLNIQVRTYN
TTIDKEHSVS LFFQQLLFTI DTLISGSYQG LEVTRTIPCT HCIQLNPTIE PYHFDLGDCI
SALEDGRSHV YCGNDPNTPV RIDYIAPDIC LKKVPTFTDD QILYERQIGE GGFGLIHKGK
LISNKSDIAI KSLILCNSNN NNNSNNNNNS NNSNNFKNNN NEDEFIEKFQ EFQHEVFIMS
NLNHPNIVKL YGLMHNPPRM VMEYVEHGDL YHLCQDKERY ASLGWTLKLR LMIDIAKGIG
YMQNQNPPIV HRDLRSPNIF LKSLDENSPV CAKIADFGLS QQSVYSVSGL LGNFQWMAPE
TIGVEDESYT EKVDTYSFAM ILFTILTGEI PFDEYSFGKM QFINLIREEN LRPTLPFDCD
PRLSNLIQLC WSTNPKKRPS FTFIIKELLE IRIDSIQTSY FDSPFLKNYP ASI
//
