ID ARLMC_PYRFU Reviewed; 409 AA.
AC Q8U2K7;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 01-MAY-2013, entry version 57.
DE RecName: Full=23S rRNA (uracil(747)-C(5))-methyltransferase;
DE EC=2.1.1.189;
DE AltName: Full=23S rRNA(m5U747)-methyltransferase;
GN OrderedLocusNames=PF0827;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and
RT P. horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
CC -!- FUNCTION: Catalyzes the formation of 5-methyl-uridine at position
CC equivalent to 747 (m5U747) in 23S rRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uracil(747) in 23S
CC rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S
CC rRNA.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA M5U
CC methyltransferase family.
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DR EMBL; AE009950; AAL80951.1; -; Genomic_DNA.
DR RefSeq; NP_578556.1; NC_003413.1.
DR ProteinModelPortal; Q8U2K7; -.
DR STRING; 186497.PF0827; -.
DR PRIDE; Q8U2K7; -.
DR EnsemblBacteria; AAL80951; AAL80951; PF0827.
DR GeneID; 1468683; -.
DR KEGG; pfu:PF0827; -.
DR eggNOG; COG2265; -.
DR HOGENOM; HOG000029870; -.
DR OMA; VECRILE; -.
DR ProtClustDB; CLSK253194; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0070041; F:rRNA (uridine-C5-)-methyltransferase activity; ISS:UniProtKB.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR001566; 23S_rRNA_MeTrfase_RlmD.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR010280; U5_MeTrfase.
DR Pfam; PF05958; tRNA_U5-meth_tr; 2.
DR TIGRFAMs; TIGR00479; rumA; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 409 23S rRNA (uracil(747)-C(5))-
FT methyltransferase.
FT /FTId=PRO_0000162055.
FT ACT_SITE 365 365 Nucleophile (By similarity).
FT METAL 61 61 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 67 67 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 70 70 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 137 137 Iron-sulfur (4Fe-4S) (By similarity).
FT BINDING 251 251 S-adenosyl-L-methionine (By similarity).
FT BINDING 277 277 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 298 298 S-adenosyl-L-methionine (By similarity).
FT BINDING 339 339 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 409 AA; 46868 MW; F6049CC3DAF85D5E CRC64;
MRGIIEDLSQ DGLGVINGIE VPFCYPGDEV RITRTRDRFG RKMASEFSLI TPSPLRQRPR
CRHYGKCGGC LWQGMKYEEQ LKFKKELFRR ITGIEAEILG SPRIWEFRNI SNFIVSVNGI
GLKEFARPKT VVDLKECPVF SNRTPLYIRA MKEFLRESGL KPWNWKEGDV HYLQVREGKF
TGEVMVNIIA HRPPEETILE YFPFADSVYW SIKRDKRDDP SGEPIHLGEK EFISEKIFGI
KYLLRPGIFF QTNSYALPLL LKAVEGFLDG SKVLDLYSGI GTFSLYLTKK GFNVVGVEIN
KTAVEVAKLS AELNSLNVEF KAKRAEEENI EGYDALILDP PRKGLGEFAG VVEKKGPENV
VYVSCNPKRF ILDFKNYLSR SYKVEDAILI DMFPHTPHVE AVIKLRKYS
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