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Database: UniProt
Entry: Q8U2K7
LinkDB: Q8U2K7
Original site: Q8U2K7 
ID   ARLMC_PYRFU             Reviewed;         409 AA.
AC   Q8U2K7;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   11-JUN-2014, entry version 62.
DE   RecName: Full=23S rRNA (uracil(747)-C(5))-methyltransferase;
DE            EC=2.1.1.189;
DE   AltName: Full=23S rRNA(m5U747)-methyltransferase;
GN   OrderedLocusNames=PF0827;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and
RT   P. horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
CC   -!- FUNCTION: Catalyzes the formation of 5-methyl-uridine at position
CC       equivalent to 747 (m5U747) in 23S rRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uracil(747) in 23S
CC       rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S
CC       rRNA.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC       methyltransferase superfamily. RNA M5U methyltransferase family.
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DR   EMBL; AE009950; AAL80951.1; -; Genomic_DNA.
DR   RefSeq; NP_578556.1; NC_003413.1.
DR   ProteinModelPortal; Q8U2K7; -.
DR   STRING; 186497.PF0827; -.
DR   PRIDE; Q8U2K7; -.
DR   EnsemblBacteria; AAL80951; AAL80951; PF0827.
DR   GeneID; 1468683; -.
DR   KEGG; pfu:PF0827; -.
DR   eggNOG; COG2265; -.
DR   HOGENOM; HOG000029870; -.
DR   OMA; GDVHYLQ; -.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0070041; F:rRNA (uridine-C5-)-methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0031167; P:rRNA methylation; ISS:UniProtKB.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR001566; 23S_rRNA_MeTrfase_RlmD.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases-like.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 2.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00479; rumA; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW   Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    409       23S rRNA (uracil(747)-C(5))-
FT                                methyltransferase.
FT                                /FTId=PRO_0000162055.
FT   ACT_SITE    365    365       Nucleophile (By similarity).
FT   METAL        61     61       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        67     67       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        70     70       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       137    137       Iron-sulfur (4Fe-4S) (By similarity).
FT   BINDING     251    251       S-adenosyl-L-methionine (By similarity).
FT   BINDING     277    277       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen (By similarity).
FT   BINDING     298    298       S-adenosyl-L-methionine (By similarity).
FT   BINDING     339    339       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   409 AA;  46868 MW;  F6049CC3DAF85D5E CRC64;
     MRGIIEDLSQ DGLGVINGIE VPFCYPGDEV RITRTRDRFG RKMASEFSLI TPSPLRQRPR
     CRHYGKCGGC LWQGMKYEEQ LKFKKELFRR ITGIEAEILG SPRIWEFRNI SNFIVSVNGI
     GLKEFARPKT VVDLKECPVF SNRTPLYIRA MKEFLRESGL KPWNWKEGDV HYLQVREGKF
     TGEVMVNIIA HRPPEETILE YFPFADSVYW SIKRDKRDDP SGEPIHLGEK EFISEKIFGI
     KYLLRPGIFF QTNSYALPLL LKAVEGFLDG SKVLDLYSGI GTFSLYLTKK GFNVVGVEIN
     KTAVEVAKLS AELNSLNVEF KAKRAEEENI EGYDALILDP PRKGLGEFAG VVEKKGPENV
     VYVSCNPKRF ILDFKNYLSR SYKVEDAILI DMFPHTPHVE AVIKLRKYS
//
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