ID Q8UY47_9INFA Unreviewed; 750 AA.
AC Q8UY47;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:AAL59149.1};
OS Influenza A virus (A/Goose/Hong Kong/385.5/2000(H5N1)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=182782 {ECO:0000313|EMBL:AAL59149.1};
RN [1] {ECO:0000313|EMBL:AAL59149.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/Goose/Hong Kong/385.5/2000 {ECO:0000313|EMBL:AAL59149.1};
RX PubMed=11739677; DOI=10.1128/JVI.76.1.118-126.2002;
RA Webster R.G., Guan Y., Peiris M., Walker D., Krauss S., Zhou N.N.,
RA Govorkova E.A., Ellis T.M., Dyrting K.C., Sit T., Perez D.R.,
RA Shortridge K.F.;
RT "Characterization of H5N1 influenza viruses that continue to circulate in
RT geese in southeastern China.";
RL J. Virol. 76:118-126(2002).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; AF398424; AAL59149.1; -; Genomic_RNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 279..476
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 43..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAL59149.1"
SQ SEQUENCE 750 AA; 85647 MW; 698D36DA77FDF72F CRC64;
LFLKVPAQNA ISTTFPYTGD PPYSHGTGTG YTMDTVNRTH QYSEKGKWTT NTETGAPQLN
PIDGPLPEDN EPSGYAQTDC VLEAMAFLEK SHPGIFENSC LETMEIVHQT RVDKLTQGRQ
TYDWTLNRNQ PAATALANTI EVFRSNGLTA NESGRLIDFL KDVMESMDKG EMEITTHFQR
KRRVRDNMTK KMVTQRTIGK KKQRLNKRSY LIRALTLNTM TKDAERGKLK RRAIATPGMQ
IRGFVYFVET LARSICEKLE QSGLPVGGNE KKAKLANVVR KMMTNSQDTE LSFTITGDNT
KWNENQNPRM FLAMITYITR KQPEWFRNVL SIAPIMFSNK MARLGKGYMF ESKSMKLRTQ
IPAEMLASID LRYFNESTRK KIEKIRPLLI DGTASLSPGM MMGMFNMLST VLGVSILNLG
QKRYTKTTYW WDGLQSSDDF ALIVNAPNHE GIQAGVDRFY RTCKLVGINM SKKKSYINRT
GTFEFTSFFY RYGFVANFSM ELPSFGVSGI NESADMSIGV TVIKNNMINN DLGPATAQMA
LQLFIKDYRY TYRCHRGDTQ IQTRRSFELK KLWEQTRSKA GLLVSDGGPN LYNIRNLHIP
EVCLKWELMD EDYQGRLCNP LNPFVSHKEI ESVNNAVVMP AHGPAKSMEY DAVATTHSWI
PKRNRSILNT SQRGILEDEQ MYQKCCNLFE KFFPSSSYRR PVGISSMVEA MVSRARIDAR
IDFESGRIKK EEFAEIMKIC STIEELRRQK
//
