ID Q8VXU1_BRACM Unreviewed; 458 AA.
AC Q8VXU1;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 08-NOV-2023, entry version 78.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN Name=fae1 {ECO:0000313|EMBL:CAC79670.1};
OS Brassica campestris (Field mustard).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3711 {ECO:0000313|EMBL:CAC79670.1};
RN [1] {ECO:0000313|EMBL:CAC79670.1}
RP NUCLEOTIDE SEQUENCE.
RA Das S., Roscoe T.J., Delseny M., Srivastava P.S., Lakshmikumaran M.;
RT "Cloning and molecular characterization of the fatty acid Elongase 1 (FAE
RT 1) gene from high and low erucic acid lines of brassica campestris and
RT brassica oleracea.";
RL Plant Sci. 162:245-250(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|PIRNR:PIRNR036417}.
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DR EMBL; Y14975; CAC79670.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8VXU1; -.
DR UniPathway; UPA00094; -.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF88; 3-KETOACYL-COA SYNTHASE 18; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..33
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 53..72
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 70..367
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 387..446
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 222
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 301
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 386
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 390
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 419
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 423
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 458 AA; 51136 MW; 3CEF309B98737EC1 CRC64;
MTSINVKLLY HYVITNLFNL CFFPLTAIVA GKAYRLTIDD LHHLYYAYLQ HNLITIAPLF
AFTVFGSVLY IATRPKPVYL VEYSCYLPPT HCRSSISKVM DIFYQVRKAD PSRIGTCDDS
SWLDFLRKIQ ERSGLGDENH GPEGLLQVPP RKTFAAAREE TEQVIIGALE NLFKNTNVSP
KDIGILVVHS SMFNPTPSLS AMVNNFKLRS NVRSFNLGGM GCSAGVIAID LAKDLLHVHK
NTYALVVSTE NITYNIYAGD NRSMMVSNCL FRVGGAAILL SNKPGDRRRS KYELVHTVRT
HTGADDYSFR CVQQGDDENG KTGVSLSKDI TDVAGRTVKK NIATLGPLIL PLSEKLLFFV
TFMGKKLFKD KIKHYYVPDF KLAIDHFCIH AGGRAVIDVL EKNLALAPID VEASRSTLHR
FGNTSSSSIW YELAYIEAKG RMKKGNKVWQ IALGSGFK
//
