GenomeNet

Database: UniProt
Entry: Q8ZD74
LinkDB: Q8ZD74
Original site: Q8ZD74 
ID   LEPA_YERPE              Reviewed;         599 AA.
AC   Q8ZD74; Q0WDH5;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-SEP-2014, entry version 98.
DE   RecName: Full=Elongation factor 4;
DE            Short=EF-4;
DE            EC=3.6.5.n1;
DE   AltName: Full=Ribosomal back-translocase LepA;
GN   Name=lepA; OrderedLocusNames=YPO2716, y1295, YP_2520;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G.,
RA   Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V.,
RA   Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M.,
RA   Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM10+ / Biovar Mediaevalis;
RX   PubMed=12142430; DOI=10.1128/JB.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C.,
RA   Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V.,
RA   Straley S.C., McDonough K.A., Nilles M.L., Matson J.S., Blattner F.R.,
RA   Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z.,
RA   Jin L., Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J.,
RA   Yang H., Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Required for accurate and efficient protein synthesis
CC       under certain stress conditions. May act as a fidelity factor of
CC       the translation reaction, by catalyzing a one-codon backward
CC       translocation of tRNAs on improperly translocated ribosomes. Back-
CC       translocation proceeds from a post-translocation (POST) complex to
CC       a pre-translocation (PRE) complex, thus giving elongation factor G
CC       a second chance to translocate the tRNAs correctly. Binds to
CC       ribosomes in a GTP-dependent manner (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + H(2)O = GDP + phosphate.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein; Cytoplasmic side (By similarity).
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. LepA
CC       subfamily.
CC   -!- SIMILARITY: Contains 1 tr-type G (guanine nucleotide-binding)
CC       domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AL590842; CAL21335.1; -; Genomic_DNA.
DR   EMBL; AE009952; AAM84869.1; -; Genomic_DNA.
DR   EMBL; AE017042; AAS62718.1; -; Genomic_DNA.
DR   PIR; AD0331; AD0331.
DR   RefSeq; NP_668618.1; NC_004088.1.
DR   RefSeq; NP_993841.1; NC_005810.1.
DR   RefSeq; YP_002347663.1; NC_003143.1.
DR   ProteinModelPortal; Q8ZD74; -.
DR   SMR; Q8ZD74; 1-555.
DR   STRING; 214092.YPO2716; -.
DR   PRIDE; Q8ZD74; -.
DR   DNASU; 1146242; -.
DR   EnsemblBacteria; AAM84869; AAM84869; y1295.
DR   EnsemblBacteria; AAS62718; AAS62718; YP_2520.
DR   GeneID; 1146242; -.
DR   GeneID; 1175546; -.
DR   GeneID; 2763941; -.
DR   KEGG; ype:YPO2716; -.
DR   KEGG; ypk:y1295; -.
DR   KEGG; ypm:YP_2520; -.
DR   eggNOG; COG0481; -.
DR   HOGENOM; HOG000020624; -.
DR   KO; K03596; -.
DR   OMA; CYTALEM; -.
DR   OrthoDB; EOG6ZKXQ4; -.
DR   BioCyc; YPES214092:GKDD-2683-MONOMER; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0043022; F:ribosome binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0045727; P:positive regulation of translation; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.240; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00071; LepA; 1.
DR   InterPro; IPR006297; EF-4.
DR   InterPro; IPR000795; EF_GTP-bd_dom.
DR   InterPro; IPR009022; EFG_III-V.
DR   InterPro; IPR000640; EFG_V.
DR   InterPro; IPR013842; LepA_GTP-bd_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR004161; Transl_elong_EFTu/EF1A_2.
DR   Pfam; PF00679; EFG_C; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF06421; LepA_C; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SMART; SM00838; EFG_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54980; SSF54980; 2.
DR   TIGRFAMs; TIGR01393; lepA; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome; GTP-binding;
KW   Hydrolase; Membrane; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN         1    599       Elongation factor 4.
FT                                /FTId=PRO_0000176381.
FT   DOMAIN        2    184       tr-type G.
FT   NP_BIND      14     19       GTP (By similarity).
FT   NP_BIND     131    134       GTP (By similarity).
SQ   SEQUENCE   599 AA;  66709 MW;  BAF32CD792DB5BDB CRC64;
     MKHIRNFSII AHIDHGKSTL SDRIIQICGG LSEREMAAQV LDSMDLERER GITIKAQSVT
     LDYHSKDGQT YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM
     EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAIR CSAKTGVGVP DVLERLVRDI
     PAPEGDPNGP LQALIIDSWF DNYLGVVSLI RIKNGSLRKG DKVKVMSTGQ SYNADRLGIF
     TPKRVDRDVL NCGEVGWLVC AIKDILGAPV GDTLTLTRNP AEKSLPGFKK VKPQVYAGLF
     PISSDDYESF RDALGKLSLN DASLFYEPES STALGFGFRC GFLGLLHMEI IQERLEREYD
     LELITTAPTV VYEVITTNQE TVYVDSPSKL PALNNIEELR EPIAECHMLL PQEYLGNVIT
     LCIEKRGTQT NMVYHGKQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQTSDM
     VRVDVLINNE RVDALALITH RDNAQYRGRD LVEKMKELIP RQQFDIAIQA AIGNHIIARS
     TVKQLRKNVL AKCYGGDVSR KKKLLQKQKD GKKRMKQVGN VELPQEAFLA ILHVGKDSK
//
DBGET integrated database retrieval system