ID TAF5L_MOUSE Reviewed; 589 AA.
AC Q91WQ5; Q8BTL5;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 27-MAR-2024, entry version 169.
DE RecName: Full=TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L {ECO:0000305};
DE Short=TAF5L {ECO:0000305};
DE AltName: Full=PCAF-associated factor 65 beta;
DE Short=PAF65-beta;
GN Name=Taf5l {ECO:0000312|MGI:MGI:1919039}; Synonyms=Paf65b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Colon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=31005419; DOI=10.1016/j.molcel.2019.03.025;
RA Seruggia D., Oti M., Tripathi P., Canver M.C., LeBlanc L.,
RA Di Giammartino D.C., Bullen M.J., Nefzger C.M., Sun Y.B.Y., Farouni R.,
RA Polo J.M., Pinello L., Apostolou E., Kim J., Orkin S.H., Das P.P.;
RT "TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC
RT Regulatory Network.";
RL Mol. Cell 74:1148.E7-1163.E7(2019).
CC -!- FUNCTION: Functions as a component of the PCAF complex. The PCAF
CC complex is capable of efficiently acetylating histones in a nucleosomal
CC context. The PCAF complex could be considered as the human version of
CC the yeast SAGA complex (By similarity). With TAF6L, acts as an
CC epigenetic regulator essential for somatic reprogramming. Regulates
CC target genes through H3K9ac deposition and MYC recruitment which
CC trigger MYC regulatory network to orchestrate gene expression programs
CC to control embryonic stem cell state (PubMed:31005419).
CC {ECO:0000250|UniProtKB:O75529, ECO:0000269|PubMed:31005419}.
CC -!- SUBUNIT: The PCAF complex is composed of a number of TBP-associated
CC factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and
CC TAF12, PCAF, and also PCAF-associated factors (PAFs), such as
CC TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription
CC coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L,
CC TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9 (By
CC similarity). {ECO:0000250|UniProtKB:O75529}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31005419}.
CC -!- SIMILARITY: Belongs to the WD repeat TAF5 family. {ECO:0000305}.
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DR EMBL; AK033477; BAC28309.1; -; mRNA.
DR EMBL; AK089463; BAC40894.1; -; mRNA.
DR EMBL; BC013550; AAH13550.1; -; mRNA.
DR CCDS; CCDS22767.1; -.
DR RefSeq; NP_598727.1; NM_133966.2.
DR RefSeq; XP_006530609.1; XM_006530546.3.
DR AlphaFoldDB; Q91WQ5; -.
DR SMR; Q91WQ5; -.
DR BioGRID; 221810; 8.
DR ComplexPortal; CPX-1024; PCAF histone acetylase complex.
DR ComplexPortal; CPX-6803; SAGA complex, KAT2B variant.
DR ComplexPortal; CPX-916; TFTC histone acetylation complex.
DR ComplexPortal; CPX-920; SAGA complex, KAT2A variant.
DR IntAct; Q91WQ5; 2.
DR MINT; Q91WQ5; -.
DR STRING; 10090.ENSMUSP00000128710; -.
DR iPTMnet; Q91WQ5; -.
DR PhosphoSitePlus; Q91WQ5; -.
DR EPD; Q91WQ5; -.
DR MaxQB; Q91WQ5; -.
DR PaxDb; 10090-ENSMUSP00000090726; -.
DR ProteomicsDB; 254812; -.
DR Pumba; Q91WQ5; -.
DR Antibodypedia; 20789; 255 antibodies from 30 providers.
DR DNASU; 102162; -.
DR Ensembl; ENSMUST00000093039.6; ENSMUSP00000090726.6; ENSMUSG00000038697.16.
DR Ensembl; ENSMUST00000165628.9; ENSMUSP00000128710.2; ENSMUSG00000038697.16.
DR GeneID; 102162; -.
DR KEGG; mmu:102162; -.
DR UCSC; uc009nww.2; mouse.
DR AGR; MGI:1919039; -.
DR CTD; 27097; -.
DR MGI; MGI:1919039; Taf5l.
DR VEuPathDB; HostDB:ENSMUSG00000038697; -.
DR eggNOG; KOG0263; Eukaryota.
DR GeneTree; ENSGT00940000153342; -.
DR HOGENOM; CLU_005884_3_0_1; -.
DR InParanoid; Q91WQ5; -.
DR OMA; RTEHVQM; -.
DR OrthoDB; 3138699at2759; -.
DR PhylomeDB; Q91WQ5; -.
DR TreeFam; TF300669; -.
DR BioGRID-ORCS; 102162; 19 hits in 83 CRISPR screens.
DR ChiTaRS; Taf5l; mouse.
DR PRO; PR:Q91WQ5; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q91WQ5; Protein.
DR Bgee; ENSMUSG00000038697; Expressed in humerus cartilage element and 260 other cell types or tissues.
DR ExpressionAtlas; Q91WQ5; baseline and differential.
DR Genevisible; Q91WQ5; MM.
DR GO; GO:0036464; C:cytoplasmic ribonucleoprotein granule; ISO:MGI.
DR GO; GO:0000123; C:histone acetyltransferase complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000124; C:SAGA complex; ISO:MGI.
DR GO; GO:0033276; C:transcription factor TFTC complex; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; NAS:ComplexPortal.
DR GO; GO:0006282; P:regulation of DNA repair; NAS:ComplexPortal.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IDA:UniProtKB.
DR GO; GO:0043484; P:regulation of RNA splicing; NAS:ComplexPortal.
DR GO; GO:1904672; P:regulation of somatic stem cell population maintenance; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR CDD; cd08044; TAF5_NTD2; 1.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 1.25.40.500; TFIID subunit TAF5, NTD2 domain; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 3.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR007582; TFIID_NTD2.
DR InterPro; IPR037264; TFIID_NTD2_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR19879:SF6; TAF5-LIKE RNA POLYMERASE II P300_CBP-ASSOCIATED FACTOR-ASSOCIATED FACTOR 65 KDA SUBUNIT 5L; 1.
DR PANTHER; PTHR19879; TRANSCRIPTION INITIATION FACTOR TFIID; 1.
DR Pfam; PF04494; TFIID_NTD2; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF160897; Taf5 N-terminal domain-like; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..589
FT /note="TAF5-like RNA polymerase II p300/CBP-associated
FT factor-associated factor 65 kDa subunit 5L"
FT /id="PRO_0000051262"
FT REPEAT 266..305
FT /note="WD 1"
FT REPEAT 340..379
FT /note="WD 2"
FT REPEAT 382..421
FT /note="WD 3"
FT REPEAT 424..463
FT /note="WD 4"
FT REPEAT 466..505
FT /note="WD 5"
FT REPEAT 508..547
FT /note="WD 6"
SQ SEQUENCE 589 AA; 65911 MW; ABD880FD73F1FCD8 CRC64;
MKRVRTEQVQ VAVSCYLKRR QYVDSEGPLK QGLRLSQTPE EMAANLTVQS ESGCANAVSA
APCQAEPQQY EVQFGRLRSF LTDSDSQYSR EVMPLLYPLF VYLHLNLVQS GPKSTVESFY
SRFHGMFLQN ASQKDVIEQL QTTQTIQDIL SNFQLRAFLD NKYVVRLQED SYNYLIRYLQ
SDNNTALCKV LAVHIHLDVQ PAKRTDYQLY ASGGSSRTEN SSLEPPEVPS PILQNEAALE
VLQESIKRVK DGPPSLTTIC FYAFYNTEQL LNTAEISSDS KLLAAGFDNS CIKLWSLRSK
KLKSEPHHVD TSRIRLACDT LEEEENEEDN TGTEMKILRG HCGPVYSTRF LADSSGLLSC
SEDMSIRYWD LGSFTNTVLY QGHAYPVWDV DISPFSLYFA SGSHDRTARL WSFDRTYPLR
IYAGHLADVD CVKFHPNSNY LATGSTDKTV RLWSAQQGNS VRLFTGHRGP VLSLSFSPNG
KYLASAGEDQ RLKLWDLASG TLFKELRGHT DSITSLAFSP DSGLIASASM DNSVRVWDIR
STCCNTPADG SSGELVGVYT GQMSNVLSVQ FMACNLLLVT GITQENQEH
//
