ID RNPH_RHIME Reviewed; 239 AA.
AC Q92SK2;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 01-MAY-2013, entry version 56.
DE RecName: Full=Ribonuclease PH;
DE Short=RNase PH;
DE EC=2.7.7.56;
DE AltName: Full=tRNA nucleotidyltransferase;
GN Name=rph; OrderedLocusNames=R00376; ORFNames=SMc01144;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.,
RA Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U.,
RA Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont
RT Sinorhizobium meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F.,
RA Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M.,
RA Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D.,
RA Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V.,
RA Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B.,
RA Ramsperger U., Surzycki R., Thebault P., Vandenbol M.,
RA Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Phosphorolytic exoribonuclease that removes nucleotide
CC residues following the -CCA terminus of tRNA and adds nucleotides
CC to the ends of RNA molecules by using nucleoside diphosphates as
CC substrates (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA(n+1) + phosphate = tRNA(n) + a nucleoside
CC diphosphate.
CC -!- SIMILARITY: Belongs to the RNase PH family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AL591688; CAC41813.1; -; Genomic_DNA.
DR RefSeq; NP_384482.1; NC_003047.1.
DR ProteinModelPortal; Q92SK2; -.
DR SMR; Q92SK2; 2-238.
DR STRING; 266834.SMc01144; -.
DR EnsemblBacteria; CAC41813; CAC41813; SMc01144.
DR GeneID; 1232010; -.
DR KEGG; sme:SMc01144; -.
DR PATRIC; 23629957; VBISinMel96828_1748.
DR eggNOG; COG0689; -.
DR HOGENOM; HOG000229516; -.
DR KO; K00989; -.
DR OMA; MLPRATG; -.
DR ProtClustDB; PRK00173; -.
DR BioCyc; SMEL266834:GJF6-387-MONOMER; -.
DR GO; GO:0000049; F:tRNA binding; IEA:HAMAP.
DR GO; GO:0009022; F:tRNA nucleotidyltransferase activity; IEA:HAMAP.
DR GO; GO:0004549; F:tRNA-specific ribonuclease activity; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR HAMAP; MF_00564; RNase_PH; 1; -.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR015847; ExoRNase_PH_dom2.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR002381; RNase_PH_bac-type.
DR InterPro; IPR018336; RNase_PH_CS.
DR Pfam; PF01138; RNase_PH; 1.
DR Pfam; PF03725; RNase_PH_C; 1.
DR SUPFAM; SSF55666; 3_ExoRNase; 1.
DR SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1.
DR TIGRFAMs; TIGR01966; RNasePH; 1.
DR PROSITE; PS01277; RIBONUCLEASE_PH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Nucleotidyltransferase; Transferase;
KW tRNA processing.
FT CHAIN 1 239 Ribonuclease PH.
FT /FTId=PRO_0000139929.
SQ SEQUENCE 239 AA; 26100 MW; 6019320CC79D6953 CRC64;
MRPSGRKTDQ MRKVSFERSF SKHAEGSCLV RFGDTHVLCT ASLEEKVPAW LRNGGKGWIT
AEYGMLPRAT GERMRREAAT GKQSGRTQEI QRLIGRSLRA VVDLPALGER QISIDCDVIQ
ADGGTRTASI TGAWIALHDC LKWMEARNMV KLEKVLKDHV AAISCGIFAN QPVIDLDYLE
DSAAETDANF VMTGSGGLVE IQGTAEGKPF SEEEFASLML LAKNGIAELV EMQKQAIAG
//
