ID Q93H84_STRAX Unreviewed; 6145 AA.
AC Q93H84;
DT 01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT 01-DEC-2001, sequence version 1.
DT 27-MAR-2024, entry version 138.
DE SubName: Full=Modular polyketide synthase {ECO:0000313|EMBL:BAB69306.1};
GN Name=pteA4 {ECO:0000313|EMBL:BAB69306.1};
OS Streptomyces avermitilis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=33903 {ECO:0000313|EMBL:BAB69306.1};
RN [1] {ECO:0000313|EMBL:BAB69306.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=11572948; DOI=10.1073/pnas.211433198;
RA Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M.,
RA Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T.,
RA Sakaki Y., Hattori M.;
RT "Genome sequence of an industrial microorganism Streptomyces avermitilis:
RT deducing the ability of producing secondary metabolites.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; AB070949; BAB69306.1; -; Genomic_DNA.
DR OMA; ASFMAPN; -.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 4.
DR CDD; cd00833; PKS; 4.
DR Gene3D; 3.30.70.3290; -; 4.
DR Gene3D; 3.40.47.10; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 4.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 4.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 4.
DR Pfam; PF16197; KAsynt_C_assoc; 4.
DR Pfam; PF00109; ketoacyl-synt; 4.
DR Pfam; PF02801; Ketoacyl-synt_C; 4.
DR Pfam; PF08659; KR; 4.
DR Pfam; PF18369; PKS_DE; 4.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00827; PKS_AT; 4.
DR SMART; SM00822; PKS_KR; 4.
DR SMART; SM00825; PKS_KS; 4.
DR SMART; SM00823; PKS_PP; 4.
DR SMART; SM01294; PKS_PP_betabranch; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 4.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 8.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 4.
DR SUPFAM; SSF53901; Thiolase-like; 4.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00606; KS3_1; 4.
DR PROSITE; PS52004; KS3_2; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 18..444
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1447..1525
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1542..1968
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2951..3026
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3044..3470
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4481..4556
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4575..5001
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 5987..6062
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 6145 AA; 642037 MW; 6A75B3EF7A7EDE95 CRC64;
MDLSQTRRQL GELEAKDTEP IAIVGMACRY PGGVASPEEL WQLVASGGDG VSGFPTDRGW
DLDTIYDPEP GRTGRTYVRE GGFLYDAGEF DPAFFGISPR EALVMDPQQR LLLQTSWEAL
ERAGIDPVTA RGTRAGVFVG TNGQDHAYLQ RQGDSDSEAH LVTANTAAVV SGRISYALGL
EGPAVTVDTA CSSSLVALHL AAQALRRGEC SLALAGGVTV MATPNTFIAF SAQRGLSPDG
RCRAFSSTAD GTGMSEGIGV LLLERLSDAR RLGHEVLAVI RGSAVNQDGA SNGLTAPNGP
SQERVIRQAL ENARLTASQV DAVEAHGTAT TLGDPIEAEA LLATYGQEHS DDRPLWLGSI
KSNIGHTQAA AGVAGVIKMV MAIRNGVLPQ TLDVDEPTAQ VDWSAGGVKL LTEAVAWPES
DHPRRAAVSS FGVSGTNAHT IIEQAPALDR EPAAEPAGAP AAGALPWVLS AKSDAALRAQ
AERLLSFLED GRSGGTGPGR SPADIGFSLA TTRTAWDRRA AVVGESLEEL TEGVRALASG
TPSAAVVRNA ARHGDKTGFL FSGQGSQRLG MGRELYDAFP VFAAAYDEIR AHLDVALDAD
AEQLNQTGCT QPALFAVEVA LFRLLESWGV RPDYVAGHSV GEIAAAHVAG VLSLGDAARL
VSARAALMQA LPAGGAMVAV QAAEDEVVPH LTHGVGIAAI NGPRSVVVSG AEDAVLAIVE
VFTQQGCKTS RLMVSHAFHS PLMDPVLEEF AEVVRGLTFS EPRIPVVANL TGRLAEAYTP
EYWVRHVREA VRFADGIETL GELGVTTFVE IGPGGVLSAL VQGCFDDGTD IVTVPSLRAD
RPEPYALVTA LAELHVSGAE LDWHAFFPGA RRVDLPTYAF QQQRYWLEAR EAETATVDAV
DAEFWASVES EDAQSLAATL GLNPEELDVV VPKLSAWRRR RREQSVVDGR RYQLAWQPLN
GLSAGDLSGS TWLFAAPEGD EWAGTVRTAL AGRGAHLIPL TVGADADRAS LTDELTRVAG
EAGSLDGVLS LLATDEIASP EHPALSQGFA QSVALAQALG DAGVDAPLWC ATRGAAATGR
SDDAPLSAVQ NQVWGLGRAL ALEHARRWGG LIDLPAAVDE RAAGRIAAVL SQGADAADGQ
EDQVAVRATG VFTARLAHAR SGVGRPWSPR GTVLITGGTG ALGGHVARWL AGAGAEHLVL
TSRRGADAPG ATALKAELEE LGARVTLAVC DVADRDALAA LLAEHTFTSV FHAAGVEQFA
PFDELTPADF ARTMAAKAHG AAHLDELLGD RELDAFVLFS SIAGVWGSGL QTAYAAGNAF
LDGLAARRRA RGLTATAIAW GPWADGGMVT DADEAHLRRR GVVTLPAALA VTAVQRALDC
DDTAVVVADI DWERFIGPFT FGRPSALLSE LPEVRRAGAV TAAVTAAPAD VGATPLAARL
AGLPEAERTH TLIDLVRAHV AAVLGHSSAA EVEPDRAFKA LGFDSLTAVE LRNKVNTATG
LALSPTLVFD YPNATALARF LQSELLGSQA VVEQEQHAAA DDEPIAIVAM SCHLPGGVDS
PEALWDLVTS GGDAISEFPS NRGWDVEALY DPDPDRPGKT YARDGGFLYG ATDFDAGFFG
ISPREALAMD PQQRLMLETS WEAFERAGIA PAQLRGSRTG VFVGMAYQGY GADVRRTPEG
VEGHRLVGGA SSVVSGRVAY TFGLEGPAVT IDTACSSSLV ALHLAMQSLR NGECTMALAG
GVTVMAGPSV FVEFSRQRGL SPDGRCRAFG ADADGTGWAE GVGMLLVERL SDARRNGHQV
LAVVRGSAVN QDGASNGLTA PNGPAQQRVI HQSLASAGLA PRDVDAVEAH GTGTRLGDPI
EAQALLATYG QGRDGDRPLW LGSLKSNIGH TQAAAGVAGV IKMVMAMRHG ILPRTLHADE
PTPHVDWSAG DVRLLTEAVD WPESDRPRRA AISSFGVSGT NAHTIIEQAP ATAELPPTPD
SGALVPWVLS GKGEPALRAQ AARLADHLDA NDGWTASDIG LSLASRETFE DRAVLLAQGS
DKLRQALTAL AAGQPAANVT LGRARSEGKV GFLFSGQGSQ RIGMGRELYE AFPVFADAYD
EVCARLEAPV DVDAETLHRT GCAQPALFAV EVALFRLLES WGVRPDYVAG HSVGEIAAAH
VAGVLSLDDA VRLVSARAAL MQALPVGGAM VAVQATEEEV LPHLTDEVGI AAINGPRSMV
LSGAGEAVTA IAEVFVGQGR KTSRLKVSHA FHSPLMDPML EEFAEVVRGL TFHQPQVPVV
SNLTGRLAEP YSPEYWVRHV REAVRFADGI ETLGELGVTT FVEIGPGGVL SGMVQDCVDE
AVTVPVLRGD RPEQQAVVTA LAELHVHGVS PDWQALFPGA HRVTLPTYAF QRERFWLDGE
GDLAAAGTPT DAADSGFWDS VEREDAESLA ATLGVSADAS LGAILPRLSA WRRQRREQSV
VDGWRYRVTW KPLGALPQPG ASGTWLLVVA GESEWTASVR TALDERGLEL VTLVAGPGTD
RGTLARELAG VGPVTGVLSL LAEDESASAG DPGLSHGLAA TLCLVQALGD VGVDVPLWCA
TRGAVATGRS DRVDRPLQSQ VWGFGRAAAL EHPRRWGGLL DLPELLDART AARVAAVLGQ
TAEDQVAVRA SGVLGRRLVR AARATGPAQG WSPRGTVLIT GGTGALGGHV ARWLAGAGAE
HLVLTSRRGP AAPGAAELVA ELEELGAAAT VVACDAADRD ALRALLAQHP VNAVVHAAGV
GDHVMIEDSD PAGFAGSVAA KAAGATHLDE LLAGQELDAF VMFSSGAGIW GGAGQGAYSA
ANAYLDALAE HRRAHGRTAL AVSWGGWAEG GMAGVGDGEE MLRRRGLPPM RPALAISALQ
QALADGETAL TVADFDWERF IGPFTVTRPS ALFSDVPEAG RALEAPAPGG AEAGNALAAK
LTGLPAAEQD RTLVDLVRTH AAAVLGHDGT AAVEPGRAFN DLGFDSLSAV ELRNKLTADT
GLKLPTTLVF DYPNATALAR FLRAELLGSR GAARQEQRTA AVGDEPIAIV AMSCRLPGGV
GSPEDLWQLV TSGGDVISGF PEDRGWNVEA LYDPDPGTPG KTYARDGGFL YDAGDFDAAL
FGISPREALA MDPQQRLLLE ASWEAFERAG IDPASLKGSR TGVFVGMSYQ GYGAGLPQVP
EGVEGHLLTG SAASVVSGRV AYSFGLEGPA VTVDTACSSS LVALHLAIQS LRNGESTMAV
AGGVNVMAVP AAFVEFSRQR GLAPDGRCKA FGAGADGTGW AEGVGVVLVE RLSDAVRNGH
EILAVVRGSA VNQDGASNGL TAPNGPAQQR VIRQALASAG LTEADVDAVE AHGTGTALGD
PIEAQALLAT YGQERPEDRP LWLGSLKSNI GHAQAASGVA GVIKMVMAMR HGVLPRTLHA
DEPTPHVDWS AGAVRLLTEA VGWPDTNRPR RAAISSFGVS GTNAHTIIEQ APVAEPAAVA
PVEVSSATVP WVLSGKSEAA LRAQAERLLT LTADGAEPSL TDVGFSLATT RAALEHRAAV
VGADHGRIAE GLRALASGAP APGAVLGRAD AGQVGFLFSG QGSQRIGMGR QLYEAFPVFA
EAYDEVCAHL DRHLDRPLRD VAFGENGHED GEELNRTAFT QPALFAHEVA VHRLLESWGV
RPDYLAGHSV GEIAAAHVAG ALALEDAAAL VTARAALMQA LPAGGAMIAV QATEDEVLPH
LTDDAGIAAI NGPQSVVVSG TEDAVAAVAD FFAREGRKIS RLTVSHAFHS PLMDPVLEDF
ARVVGGLRFE TPRTPVVSNL TGELIDAYTA DYWVRHVREA VRFADGIRTL GELGVTTFVE
TGPGAVLSAM AQGCLDGAVA VASARSGSPE PEAITGALAQ LHVHGVPVDW RAFFAGRGAR
RADLPTYAFQ HQRYWLETTA PTAAAGTDAV ETGFWETVER EDAQSLAATL ELPVEQLDAV
LPRLSAWRRQ QRRESAVEGW SYRTGWKPLS GLRAHELRGN WLFLVTEDTE AFEAFEWTAA
VADGLAARGA RLVRVTVDPA ADRGALLRQL GEAVRESPLD GVISLLGIDE RHHAGHPALS
VGTVLTLALV QALGDAGIDA PLWALTRSAM STGRADAVPS AAQNAVWGLG RVAALEHAPR
WGGLIDLPDT VDDRIAGRLA AVLAQSAEDQ VAIRDRGVFG RRLSHTAARR NTRAAQGWSP
RGTVLITGGT GALGGHVARW LAGAGAEHLV LTSRRGPDAP GAAGLVAELE ATGVRVTVAA
CDVADREALA ALLAEHPVNA VVHTAGVDHM EPLEAMTPGA CADVLSAKAA GALHLDALLA
GQELDAFVLF SSIAGVWGSG HQAAYAAANA LLDGLAERRR AQGLPATAVA WGPWAGGGMA
EADGADERLR RRGLIPMPAA LAVAGLRRAL DSGEATATVA DVDWERFLPP FTVGRPSALL
GDLPEAERIL TADSTAGGPG TAAASPLADR LARMPETEQH ALLVDLVRTH AAAVLGHNNA
GEVEADRAFK DLGFDSLTAV ELRNKLKAET GLALPPTLVF DHPNAQALAR QLRTELTGLT
IAAAPEAAAA PAEDEEPIAI VGMACRYPGG VRSPEDLWHL VAAGGDAVGD FPADRGWDVE
DIYDPDPDAR GKTYARRGGF LYEAGEFDPG FFGISPREAV AMDPQQRLLL ETTWETFERA
GIDAESVRGS RTGVFVGSGY QDYAARAFNA IDDSEGFFGT GNSASIMSGR IAYTFGLEGP
AVTVDTACSS SLVALHWAIQ ALRNGECSMA LAGGVMVMST PRAFVEFSRQ RGLAPDGRCK
AFGADADGTG WAEGVGMLLV ERLSDARRNG HQVLAVVRGS AVNQDGASNG LTAPNGPAQQ
RVIRQALTSA GLTAADIDAV EAHGTGTRLG DPIEAQALLA TYGQERPAGQ PLWLGSLKSN
IGHTQAAAGV AGVIKMVMAM RHGVLPQTLH VDEPTPHVDW SAGDVRLLTE AVAWPESERP
RRAAVSSFGV SGTNAHTIIE QAPPVPAEEA TVTPAPALLP WVLSAKSSAA LRDQARRLLA
FVEDGMSLAD VGFSLATTRS ALRHRAAVIG ESGDGFRRGL GSLAAGMPSP GVVQGRPGGK
VGFLFSGQGS QRIGMGRELY EAFPVFADAY DEVCARLEAP VDVDAETLHR TGCAQPALFA
VEVALFRLLE SWGVRPDYVA GHSVGEIAAV HVAGVLSLDD AVKLVSARAA LMQALPAGGA
MVAVQATEDE VLPHLTDGVG MAAINGPRSV VVSGAEDAVL EIAEVFTRQG RKTSRLKVSH
AFHSPLMDPM LEEFAEVVRG LTFHQPQVPV VSNLTGRLAE PYSPEYWVRH VREAVRFADG
IETLGELGVT TFVEIGPGGV LSGMVQDCVD EAVTVPVLRG DRPEQQAVVT ALAHLHTHGM
SVDWNVFFAG ARKADLPTYA FQHEPYWVDA PQIAAAAEAE PVDAEFWETV ERGDLQALAE
TLGVGAGDKF GDVLPRLSSW RRQRKEQSTV DTWRYGESWK GIPDRVPEGP ARIGLWLVPV
SAAQLGDDWV TACLRGLTER GLAPLPVPVE PATGREDLAA QLTRAAGDET VAGVLSLLAV
DCGAAPAAAA LTVGVALSVL LVQALGDAGI GAPLWCVTRN AVGVSTADEA TDPYQSQVWG
LGRVAALEHA PRWGGLIDLP DTVDDRIAGR LAAVLGQSAE DQVAIRDRGL FVRRLSRLPA
ALSERSWSPR GTVLITGGTG ALGGHVARWL AGAGAEHLVL TSRRGPAAPG AAGLKAELEE
AGVRVTVAAC DVADREALAV LLAEHPVNAV VHTAGTAEAG MLAETSLGDF AATVAAKALG
AVHLHELLGD QELDAFVLFS SISGVWGGGG QAAYSAANAF LDGLAQHRRA RGLAATAIAW
GPWGEGGMVA DAGDEERLRQ RGLSVLRPDR AISALHGALS GGDGTVTVAD VDWERFVVPF
TIGRPSALLS DLPEVGDALA EKRTERAAEP SALKAKLSTL SEGERQRLLV DTVCAHAAAV
LGHSGSAAVD PDLAFRDLGF DSLTAVELRN LLSADTGLTL PATLVFDHPT PEAIARYLRS
ELADETGPEE VSVFSELDRL ESVISTAASA EQTVRSGVRR RLQELLSLVN SAGEQGAESA
DAQRQLQDAT IDDIFDLIDQ DLEIS
//
