ID Q93TJ6_BACIU Unreviewed; 499 AA.
AC Q93TJ6;
DT 01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT 01-DEC-2001, sequence version 1.
DT 24-JAN-2024, entry version 100.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361153};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361153};
GN Name=cel {ECO:0000313|EMBL:AAK39540.1};
GN Synonyms=celW {ECO:0000313|EMBL:AHZ62779.1}, eglS
GN {ECO:0000313|EMBL:ADP05286.1};
OS Bacillus subtilis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=1423 {ECO:0000313|EMBL:AAK39540.1};
RN [1] {ECO:0000313|EMBL:AAK39540.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Y106 {ECO:0000313|EMBL:AAK39540.1};
RA Qi M., Wang P., Liu X., Qu Y.;
RT "The cloning and expression of a cellulase from Bacillus subtilis Y106.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADP05286.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Shu-3 {ECO:0000313|EMBL:ADP05286.1};
RA Zhang Y.;
RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ADM12805.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BEC-1 {ECO:0000313|EMBL:ADM12805.1};
RA Zhu C., Xu Z., Song R.;
RT "The endoglucanase from Bacillus subtilis BEC-1 bears halo-tolerant,
RT acidophilic and dithiothreitol-stimulated enzyme activity.";
RL World J. Microbiol. Biotechnol. 27:2863-2871(2011).
RN [4] {ECO:0000313|EMBL:AIV00153.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Ho27 {ECO:0000313|EMBL:AIV00153.1};
RA Ibrahim A.M., Ismael H.M., Helal G.E.;
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AHZ62779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WL001 {ECO:0000313|EMBL:AHZ62779.1};
RA Wang L., Chen Y.;
RT "Coexpression and secretion of endoglucanase and phytase genes in
RT Lactobacillus reuteri.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361153};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; AF355629; AAK39540.1; -; Genomic_DNA.
DR EMBL; HQ000093; ADM12805.1; -; Genomic_DNA.
DR EMBL; HM470252; ADP05286.1; -; Genomic_DNA.
DR EMBL; KJ528404; AHZ62779.1; -; Genomic_DNA.
DR EMBL; KF792058; AIV00153.1; -; Genomic_DNA.
DR RefSeq; WP_046381055.1; NZ_NJRC01000007.1.
DR AlphaFoldDB; Q93TJ6; -.
DR SMR; Q93TJ6; -.
DR CAZy; CBM3; Carbohydrate-Binding Module Family 3.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.710; Endoglucanase-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR001956; CBM3.
DR InterPro; IPR036966; CBM3_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR34142:SF1; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR Pfam; PF00942; CBM_3; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SMART; SM01067; CBM_3; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR PROSITE; PS51172; CBM3; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361153};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361153};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153, ECO:0000313|EMBL:ADP05286.1};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|RuleBase:RU361153}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..499
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5007715582"
FT DOMAIN 350..499
FT /note="CBM3"
FT /evidence="ECO:0000259|PROSITE:PS51172"
SQ SEQUENCE 499 AA; 55252 MW; 587A687584B7E5BE CRC64;
MKRSISIFIT CLLITLLTMG GMLASPASAA GTKTPVAKNG QLSIKGTQLV NRDGKAVQLK
GISSHGLQWY GEYVNKDSLK WLRDDWGITV FRAAMYTADG GYIDNPSVKN KVKEAVEAAK
ELGIYVIIDW HILNDGNPNH NKEKAKEFFK EMSSLYGNTP NVIYEIANEP NGDVNWKRDI
KPYAEEVISV IRKNDPDNII IVGTGTWSQD VNDAADDQLK DANVMYALHF YAGTHGQFLR
DKANYALSKG APIFVTEWGT SDASGNGGVF LDQSREWLKY LDSKTISWVN WNLSDKQESS
SALKPGASKT GGWQLSDLSA SGTFVRENIL GTKDSTKDIP ETPAKDKPTQ ENGISVQYRA
GDGSMNSNQI RPQLQIKNNG NTTVDLKDVT ARYWYKAKNK GQNFDCDYAQ IGCGNVTHKF
VTLHKPKQGA DTYLELGFKN GTLAPGASTG NIQLRLHNDD WSNYAQSGDY SFFKSNTFKT
TKKITLYDQG KLIWGTEPN
//
