ID Q94C08_ORYSA Unreviewed; 665 AA.
AC Q94C08;
DT 01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2003, sequence version 2.
DT 24-JAN-2024, entry version 72.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
OS Oryza sativa (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=4530 {ECO:0000313|EMBL:AAK72492.2};
RN [1] {ECO:0000313|EMBL:AAK72492.2}
RP NUCLEOTIDE SEQUENCE.
RA Fu R.-H., Sung H.-Y., Wang A.-Y.;
RT "Cloning of rice invertase cDNA.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000256|ARBA:ARBA00000094};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000256|ARBA:ARBA00004191}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; AY037870; AAK72492.2; -; mRNA.
DR AlphaFoldDB; Q94C08; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF84; ACID BETA-FRUCTOFURANOSIDASE; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT TRANSMEM 32..54
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 17..117
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 125..454
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 475..649
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 665 AA; 73412 MW; 5EE2D4B1CD6AACC0 CRC64;
MVPVSTKISP LLEVVTPIPQ EDRKRQADLK EVPAIVASAL ILLALATSIL AWALTEVESP
GEVTSGEDQT EPVVPDMEMA EPSKGVSEGV TERTYSGVLL ASGHATSTEY VWSNVMLVWQ
RTVFHFQPEK NWMNDPNGPL YHKGWYHIFY QWNPEGAAWG NKISWGHAVD SKDLLHWYHL
PWAMYPDQPY DLNGVWTGSA TTLPDGKLAM LYTGETEDEL VQDQCLADPV NPTDPLLVDW
VKYSVNPVIY PPPGIGVKDF RDPTTACRSP AGNDTRLITI GSKLGKTGIS LVYETTNFTT
FKLLYGVLHA VPGTGMWECV DLYPVSTKSV IVITQENGLD TSVHGLGVKH VLKTSFDDDK
HDYYALGTYD LQADTFVPDD PDLDVGIGLR LDYGKYYAAR TFYNQNKQRR ILWGWIGETD
LEAVDPMKGW ASLQAIPRSI VFDKKTGTNV LQRPEEEVES WSTRSKTFHD ISFEPGSVVP
IHVTGATQLD ITASFEVDET LLETTSESHD AGYDCSNSGG AGTRGSLGPF GLLVVADEKL
SELTPVYLYV AKGGEGKAKA HLCTYQTRSS MASGVEKDVY GSAVPVLDGE NYSARILTDH
SIVESFAQAG RTSVRSRDYP TKDIYGAARS FFFNNATEAS VRASLKAWQT KSFIRPYPFI
PDPKS
//
