ID   Q94KV9_BRANA            Unreviewed;       174 AA.
AC   Q94KV9;
DT   01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   RecName: Full=thioglucosidase {ECO:0000256|ARBA:ARBA00012250};
DE            EC=3.2.1.147 {ECO:0000256|ARBA:ARBA00012250};
DE   AltName: Full=Sinigrinase {ECO:0000256|ARBA:ARBA00032643};
DE   AltName: Full=Thioglucosidase {ECO:0000256|ARBA:ARBA00032797};
DE   Flags: Fragment;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708 {ECO:0000313|EMBL:AAK49403.1};
RN   [1] {ECO:0000313|EMBL:AAK49403.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Fourmann M., Froger N., Brunel D.;
RT   "Amplified consensus gene markers: Tools for designing a genetic map of
RT   Arabidopsis-known-function genes in Brassica.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC       thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC       and any of the products: thiocyanates, isothiocyanates, nitriles,
CC       epithionitriles or oxazolidine-2-thiones.
CC       {ECO:0000256|ARBA:ARBA00003014}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC         Evidence={ECO:0000256|ARBA:ARBA00034026};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC       {ECO:0000256|ARBA:ARBA00010838, ECO:0000256|RuleBase:RU003690}.
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DR   EMBL; AF261099; AAK49403.1; -; Genomic_DNA.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353:SF235; BETA-GLUCOSIDASE 21-RELATED; 1.
DR   PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000313|EMBL:AAK49403.1}.
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:AAK49403.1"
FT   NON_TER         174
FT                   /evidence="ECO:0000313|EMBL:AAK49403.1"
SQ   SEQUENCE   174 AA;  19833 MW;  433C58D174FF5695 CRC64;
     DIPQDLEDEY GGFLSDKIVK DFREYADFVF QEYGGKVKHW ITFNEPWVYS HAGYDIGKKA
     PGRCSTYVNP KCQDGRSGYE AYLVSHNLLN SHAEXVEAFR KCEKCKGGKI GIAHSPAWFE
     PHDFDDSQDG ASIDRALDFM MGWHLDTTTF GDYPQIMKDI VGHRLPQFTN AQKA
//