ID Q99Y24_STRP1 Unreviewed; 526 AA.
AC Q99Y24; Q48WN4;
DT 01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2001, sequence version 1.
DT 27-MAR-2024, entry version 146.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=hsdM {ECO:0000313|EMBL:AAK34614.1};
GN OrderedLocusNames=SPy_1906 {ECO:0000313|EMBL:AAK34614.1};
OS Streptococcus pyogenes serotype M1.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=301447 {ECO:0000313|EMBL:AAK34614.1, ECO:0000313|Proteomes:UP000000750};
RN [1] {ECO:0000313|EMBL:AAK34614.1, ECO:0000313|Proteomes:UP000000750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700294 / SF370 / Serotype M1
RC {ECO:0000313|Proteomes:UP000000750};
RX PubMed=11296296; DOI=10.1073/pnas.071559398;
RA Ferretti J.J., McShan W.M., Adjic D., Savic D., Savic G., Lyon K.,
RA Primeaux C., Sezate S.S., Surorov A.N., Kenton S., Lai H., Lin S., Qian Y.,
RA Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L., White J., Yuan X.,
RA Clifton S.W., Roe B.A., McLaughlin R.E.;
RT "Complete genome sequence of an M1 strain of Streptococcus pyogenes.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; AE004092; AAK34614.1; -; Genomic_DNA.
DR RefSeq; NP_269893.1; NC_002737.2.
DR AlphaFoldDB; Q99Y24; -.
DR REBASE; 11233; M.Spy5005I.
DR REBASE; 5131; M.Spy370I.
DR PaxDb; 1314-HKU360_01746; -.
DR DNASU; 901592; -.
DR KEGG; spy:SPy_1906; -.
DR PATRIC; fig|160490.10.peg.1655; -.
DR HOGENOM; CLU_013049_0_2_9; -.
DR OMA; YSKFHIA; -.
DR Proteomes; UP000000750; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000313|EMBL:AAK34614.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000000750};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 8..160
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 171..476
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 489..516
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 526 AA; 59499 MW; BC769721E578B85F CRC64;
MAEKTTSLRQ ALWHSADQLR GQMDANDYKN YLLGLIFYKH LSDKLLLAVC DNLEKHFNTF
TEAQKIFEDA YQDEGLKDDL ISVVTGDLGY FIEPTLTFEK LIQDVYHNTF QLESLAQGFR
DIEQSGEDFE NLFEDIDLYS KKLGSTPQKQ NQTISNVMKT LNEIDFEAVD GDTLGDAYEY
LIGEFASESG KKAGEFYTPQ AVSHLMTQIV FLGREDQKGM TLYDPAMGSG SLLLNAKKYS
NQSDTVSYYG QEINTSTYNL ARMNMMLHGV AIENQHLSNA DTLDADWPTD EPINFDGVLM
NPPYSLKWSA TAGFLTDPRF SSYGVLAPKS KADFAFLLHG FYHLKNTGTM AIVLPHGVLF
RGAAEGKIRQ KLLEQGAIDT IIGLPSNIFY NTSIPTTIII LKKNRTNKDV FFIDASKEFD
KGKNQNTMTD NHIKKILDAY KSRDNSDKFS YLASFDEIIE NDYNLNIPRY VDTFEEVPVK
PLPELAKQLS DIDQEIAKTN AKLDQLMKQL VGTTKEAQDE LDTFRN
//
