ID NOL10_HUMAN Reviewed; 688 AA.
AC Q9BSC4; A8K3R5; B4DLV0; Q53RC9; Q96TA5; Q9H7Y7; Q9H855;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 27-MAR-2024, entry version 173.
DE RecName: Full=Nucleolar protein 10;
GN Name=NOL10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC TISSUE=Brain, and Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=12429849; DOI=10.1091/mbc.e02-05-0271;
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT "Functional proteomic analysis of human nucleolus.";
RL Mol. Biol. Cell 13:4100-4109(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-475; THR-481 AND SER-514, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12429849}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9BSC4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BSC4-2; Sequence=VSP_015456;
CC Name=3;
CC IsoId=Q9BSC4-3; Sequence=VSP_015454, VSP_015455, VSP_015457,
CC VSP_015458;
CC Name=4;
CC IsoId=Q9BSC4-4; Sequence=VSP_045122;
CC -!- SIMILARITY: Belongs to the WD repeat NOL10/ENP2 family. {ECO:0000305}.
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DR EMBL; AK024000; BAB14765.1; -; mRNA.
DR EMBL; AK024137; BAB14836.1; -; mRNA.
DR EMBL; AK290680; BAF83369.1; -; mRNA.
DR EMBL; AK297169; BAG59662.1; -; mRNA.
DR EMBL; AC007314; AAK52071.2; -; Genomic_DNA.
DR EMBL; AC092687; AAY24068.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00955.1; -; Genomic_DNA.
DR EMBL; BC005125; AAH05125.1; -; mRNA.
DR CCDS; CCDS1673.2; -. [Q9BSC4-1]
DR CCDS; CCDS58697.1; -. [Q9BSC4-4]
DR CCDS; CCDS58698.1; -. [Q9BSC4-2]
DR RefSeq; NP_001248321.1; NM_001261392.1. [Q9BSC4-4]
DR RefSeq; NP_001248323.1; NM_001261394.1. [Q9BSC4-2]
DR RefSeq; NP_079170.2; NM_024894.3. [Q9BSC4-1]
DR PDB; 7MQ8; EM; 3.60 A; NW=1-688.
DR PDB; 7MQ9; EM; 3.87 A; NW=1-688.
DR PDBsum; 7MQ8; -.
DR PDBsum; 7MQ9; -.
DR AlphaFoldDB; Q9BSC4; -.
DR EMDB; EMD-23936; -.
DR EMDB; EMD-23937; -.
DR SMR; Q9BSC4; -.
DR BioGRID; 123023; 192.
DR CORUM; Q9BSC4; -.
DR IntAct; Q9BSC4; 31.
DR MINT; Q9BSC4; -.
DR STRING; 9606.ENSP00000371101; -.
DR iPTMnet; Q9BSC4; -.
DR PhosphoSitePlus; Q9BSC4; -.
DR SwissPalm; Q9BSC4; -.
DR BioMuta; NOL10; -.
DR SWISS-2DPAGE; Q9BSC4; -.
DR EPD; Q9BSC4; -.
DR jPOST; Q9BSC4; -.
DR MassIVE; Q9BSC4; -.
DR MaxQB; Q9BSC4; -.
DR PaxDb; 9606-ENSP00000371101; -.
DR PeptideAtlas; Q9BSC4; -.
DR ProteomicsDB; 4565; -.
DR ProteomicsDB; 78875; -. [Q9BSC4-1]
DR ProteomicsDB; 78876; -. [Q9BSC4-2]
DR ProteomicsDB; 78877; -. [Q9BSC4-3]
DR Pumba; Q9BSC4; -.
DR Antibodypedia; 26703; 125 antibodies from 20 providers.
DR DNASU; 79954; -.
DR Ensembl; ENST00000345985.7; ENSP00000263837.4; ENSG00000115761.17. [Q9BSC4-2]
DR Ensembl; ENST00000381685.10; ENSP00000371101.5; ENSG00000115761.17. [Q9BSC4-1]
DR Ensembl; ENST00000538384.5; ENSP00000439663.1; ENSG00000115761.17. [Q9BSC4-4]
DR GeneID; 79954; -.
DR KEGG; hsa:79954; -.
DR MANE-Select; ENST00000381685.10; ENSP00000371101.5; NM_024894.4; NP_079170.2.
DR UCSC; uc002rap.4; human. [Q9BSC4-1]
DR AGR; HGNC:25862; -.
DR CTD; 79954; -.
DR DisGeNET; 79954; -.
DR GeneCards; NOL10; -.
DR HGNC; HGNC:25862; NOL10.
DR HPA; ENSG00000115761; Low tissue specificity.
DR MIM; 616197; gene.
DR neXtProt; NX_Q9BSC4; -.
DR OpenTargets; ENSG00000115761; -.
DR PharmGKB; PA142671257; -.
DR VEuPathDB; HostDB:ENSG00000115761; -.
DR eggNOG; KOG2321; Eukaryota.
DR GeneTree; ENSGT00390000007900; -.
DR HOGENOM; CLU_009923_2_0_1; -.
DR InParanoid; Q9BSC4; -.
DR OMA; CFDSEVT; -.
DR OrthoDB; 152730at2759; -.
DR PhylomeDB; Q9BSC4; -.
DR TreeFam; TF105808; -.
DR PathwayCommons; Q9BSC4; -.
DR SignaLink; Q9BSC4; -.
DR BioGRID-ORCS; 79954; 800 hits in 1129 CRISPR screens.
DR ChiTaRS; NOL10; human.
DR GenomeRNAi; 79954; -.
DR Pharos; Q9BSC4; Tdark.
DR PRO; PR:Q9BSC4; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9BSC4; Protein.
DR Bgee; ENSG00000115761; Expressed in sperm and 186 other cell types or tissues.
DR ExpressionAtlas; Q9BSC4; baseline and differential.
DR Genevisible; Q9BSC4; HS.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR040382; NOL10/Enp2.
DR InterPro; IPR012580; NUC153.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR14927:SF0; NUCLEOLAR PROTEIN 10; 1.
DR PANTHER; PTHR14927; UNCHARACTERIZED; 1.
DR Pfam; PF08159; NUC153; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Coiled coil; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..688
FT /note="Nucleolar protein 10"
FT /id="PRO_0000051101"
FT REPEAT 44..82
FT /note="WD 1"
FT REPEAT 88..124
FT /note="WD 2"
FT REPEAT 127..163
FT /note="WD 3"
FT REPEAT 170..205
FT /note="WD 4"
FT REPEAT 219..258
FT /note="WD 5"
FT REPEAT 262..300
FT /note="WD 6"
FT REPEAT 304..341
FT /note="WD 7"
FT REGION 529..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 645..688
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 423..446
FT /evidence="ECO:0000255"
FT COILED 514..589
FT /evidence="ECO:0000255"
FT COILED 640..673
FT /evidence="ECO:0000255"
FT COMPBIAS 651..665
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..688
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 475
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 481
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 514
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..150
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_015454"
FT VAR_SEQ 71..96
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_045122"
FT VAR_SEQ 151..154
FT /note="FVGA -> MTTQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_015455"
FT VAR_SEQ 253..302
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_015456"
FT VAR_SEQ 343..345
FT /note="VLG -> PSL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_015457"
FT VAR_SEQ 346..688
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_015458"
FT VARIANT 635
FT /note="D -> N (in dbSNP:rs2287059)"
FT /id="VAR_060041"
FT CONFLICT 494
FT /note="F -> L (in Ref. 1; BAB14836)"
FT /evidence="ECO:0000305"
FT CONFLICT 570
FT /note="Q -> R (in Ref. 1; BAB14836)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 688 AA; 80302 MW; 27C3C9E0D7A4FA33 CRC64;
MQVSSLNEVK IYSLSCGKSL PEWLSDRKKR ALQKKDVDVR RRIELIQDFE MPTVCTTIKV
SKDGQYILAT GTYKPRVRCY DTYQLSLKFE RCLDSEVVTF EILSDDYSKI VFLHNDRYIE
FHSQSGFYYK TRIPKFGRDF SYHYPSCDLY FVGASSEVYR LNLEQGRYLN PLQTDAAENN
VCDINSVHGL FATGTIEGRV ECWDPRTRNR VGLLDCALNS VTADSEINSL PTISALKFNG
ALTMAVGTTT GQVLLYDLRS DKPLLVKDHQ YGLPIKSVHF QDSLDLILSA DSRIVKMWNK
NSGKIFTSLE PEHDLNDVCL YPNSGMLLTA NETPKMGIYY IPVLGPAPRW CSFLDNLTEE
LEENPESTVY DDYKFVTKKD LENLGLTHLI GSPFLRAYMH GFFMDIRLYH KVKLMVNPFA
YEEYRKDKIR QKIEETRAQR VQLKKLPKVN KELALKLIEE EEEKQKSTWK KKVKSLPNIL
TDDRFKVMFE NPDFQVDEES EEFRLLNPLV SKISEKRKKK LRLLEQQELR EKEEEEEPEG
KPSDAESSES SDDEKAWVEE VRKQRRLLQQ EEKVKRQERL KEDQQTVLKP QFYEIKAGEE
FRSFKDSATK QKLMNKTLED RLKIEAKNGT LSVSDTTVGS KQLTFTLKRS EQQKKQQEAE
KLHRQERKRL RRSAGHLKSR HKRGRSFH
//
