ID PPAT_HUMAN Reviewed; 426 AA.
AC Q9BZG2; C0H3P7; Q9BZG3; Q9BZG4;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 29-MAY-2013, entry version 77.
DE RecName: Full=Testicular acid phosphatase;
DE EC=3.1.3.2;
DE Flags: Precursor;
GN Name=ACPT;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3), INDUCTION,
RP AND TISSUE SPECIFICITY.
RX PubMed=11414767; DOI=10.1006/geno.2001.6556;
RA Yousef G.M., Diamandis M., Jung K., Diamandis E.P.;
RT "Molecular cloning of a novel human acid phosphatase gene (ACPT) that
RT is highly expressed in the testis.";
RL Genomics 74:385-395(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND GLYCOSYLATION.
RC TISSUE=Testis;
RX PubMed=15219672; DOI=10.1016/j.neuroscience.2004.04.060;
RA Fleisig H., El-Din El-Husseini A., Vincent S.R.;
RT "Regulation of ErbB4 phosphorylation and cleavage by a novel histidine
RT acid phosphatase.";
RL Neuroscience 127:91-100(2004).
CC -!- FUNCTION: Dephosphorylates receptor tyrosine-protein kinase erbB-4
CC and inhibits the ligand-induced proteolytic cleavage.
CC -!- CATALYTIC ACTIVITY: A phosphate monoester + H(2)O = an alcohol +
CC phosphate.
CC -!- SUBUNIT: Homodimer (Probable).
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane
CC protein (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9BZG2-1; Sequence=Displayed;
CC Name=2; Synonyms=Variant 3;
CC IsoId=Q9BZG2-2; Sequence=VSP_021494;
CC Name=3; Synonyms=Truncated variant 1, Truncated variant 2;
CC IsoId=Q9BZG2-3; Sequence=VSP_021493, VSP_021495;
CC -!- TISSUE SPECIFICITY: Expressed mainly in the testis. Also expressed
CC in the brain where they are enriched at the postsynaptic sites.
CC Expressed at lower levels in the trachea, prostate, bone marrow,
CC spinal cord, colon, fetal brain, heart, thymus, fetal liver,
CC spleen, leukocytes, ovary, small intestine, pancreas and skeletal
CC muscle. Expression is significantly lower in testicular cancer
CC tissues than in normal testicular tissues. Isoform 3 is expressed
CC in the testis, trachea, prostate and bone marrow.
CC -!- INDUCTION: Up-regulated by mibolerone (a synthetic androgen) and
CC dihydrotestosterone (DHT) and is down-regulated by estrogen and
CC progestin.
CC -!- PTM: Glycosylated (Probable).
CC -!- SIMILARITY: Belongs to the histidine acid phosphatase family.
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DR EMBL; AF321918; AAK09393.1; -; Genomic_DNA.
DR EMBL; AF321918; AAK09394.1; -; Genomic_DNA.
DR EMBL; AF321918; AAK09395.1; -; Genomic_DNA.
DR EMBL; AF321918; AAK09396.1; -; Genomic_DNA.
DR IPI; IPI00001603; -.
DR IPI; IPI00011500; -.
DR IPI; IPI00289055; -.
DR RefSeq; NP_149059.1; NM_033068.2.
DR UniGene; Hs.293394; -.
DR ProteinModelPortal; Q9BZG2; -.
DR STRING; 9606.ENSP00000270593; -.
DR PhosphoSite; Q9BZG2; -.
DR DMDM; 74717749; -.
DR PaxDb; Q9BZG2; -.
DR PRIDE; Q9BZG2; -.
DR DNASU; 93650; -.
DR Ensembl; ENST00000270593; ENSP00000270593; ENSG00000142513.
DR Ensembl; ENST00000270594; ENSP00000270594; ENSG00000142513.
DR GeneID; 93650; -.
DR KEGG; hsa:93650; -.
DR UCSC; uc002pta.1; human.
DR CTD; 93650; -.
DR GeneCards; GC19P051293; -.
DR HGNC; HGNC:14376; ACPT.
DR MIM; 606362; gene.
DR neXtProt; NX_Q9BZG2; -.
DR PharmGKB; PA24450; -.
DR eggNOG; NOG85977; -.
DR HOGENOM; HOG000231439; -.
DR HOVERGEN; HBG002203; -.
DR InParanoid; Q9BZG2; -.
DR KO; K14410; -.
DR OMA; ISLFYRN; -.
DR OrthoDB; EOG43FGX6; -.
DR PhylomeDB; Q9BZG2; -.
DR GenomeRNAi; 93650; -.
DR NextBio; 78184; -.
DR Bgee; Q9BZG2; -.
DR CleanEx; HS_ACPT; -.
DR Genevestigator; Q9BZG2; -.
DR GermOnline; ENSG00000142513; Homo sapiens.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0003993; F:acid phosphatase activity; IEA:EC.
DR InterPro; IPR000560; His_Pase_superF_clade-2.
DR Pfam; PF00328; His_Phos_2; 1.
DR PROSITE; PS00616; HIS_ACID_PHOSPHAT_1; 1.
DR PROSITE; PS00778; HIS_ACID_PHOSPHAT_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Complete proteome; Disulfide bond; Glycoprotein;
KW Hydrolase; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1 26 Potential.
FT CHAIN 27 426 Testicular acid phosphatase.
FT /FTId=PRO_0000259643.
FT TOPO_DOM 27 393 Extracellular (Potential).
FT TRANSMEM 394 414 Helical; (Potential).
FT TOPO_DOM 415 426 Cytoplasmic (Potential).
FT ACT_SITE 41 41 Nucleophile (By similarity).
FT ACT_SITE 289 289 Proton donor (By similarity).
FT CARBOHYD 191 191 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 269 269 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 330 330 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 339 339 N-linked (GlcNAc...) (Potential).
FT DISULFID 159 378 By similarity.
FT DISULFID 214 312 By similarity.
FT DISULFID 353 357 By similarity.
FT VAR_SEQ 39 92 FRHGDRAPLASYPMDPHKEVASTLWPRGLGQLTTEGVRQQL
FT ELGRFLRSRYEAF -> RRPHPGLPLAPPGLALTSPVPRYS
FT AMATGPRWPPTPWTHTRRWPPPCGHEAWAS (in
FT isoform 3).
FT /FTId=VSP_021493.
FT VAR_SEQ 91 183 Missing (in isoform 2).
FT /FTId=VSP_021494.
FT VAR_SEQ 93 426 Missing (in isoform 3).
FT /FTId=VSP_021495.
SQ SEQUENCE 426 AA; 46090 MW; BE930398041DB061 CRC64;
MAGLGFWGHP AGPLLLLLLL VLPPRALPEG PLVFVALVFR HGDRAPLASY PMDPHKEVAS
TLWPRGLGQL TTEGVRQQLE LGRFLRSRYE AFLSPEYRRE EVYIRSTDFD RTLESAQANL
AGLFPEAAPG SPEARWRPIP VHTVPVAEDK LLRFPMRSCP RYHELLREAT EAAEYQEALE
GWTGFLSRLE NFTGLSLVGE PLRRAWKVLD TLMCQQAHGL PLPAWASPDV LRTLAQISAL
DIGAHVGPPR AAEKAQLTGG ILLNAILANF SRVQRLGLPL KMVMYSAHDS TLLALQGALG
LYDGHTPPYA ACLGFEFRKH LGNPAKDGGN VTVSLFYRND SAHLPLPLSL PGCPAPCPLG
RFYQLTAPAR PPAHGVSCHG PYEAAIPPAP VVPLLAGAVA VLVALSLGLG LLAWRPGCLR
ALGGPV
//
