ID Q9D3K4_MOUSE Unreviewed; 507 AA.
AC Q9D3K4;
DT 01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2001, sequence version 1.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Kunitz-type protease inhibitor 1 {ECO:0008006|Google:ProtNLM};
GN Name=Spint1 {ECO:0000313|MGI:MGI:1338033};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|EMBL:BAB30697.1};
RN [1] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA Carninci P., Hayashizaki Y.;
RT "High-efficiency full-length cDNA cloning.";
RL Methods Enzymol. 303:19-44(1999).
RN [2] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=11042159; DOI=10.1101/gr.145100;
RA Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT full-length cDNA libraries for rapid discovery of new genes.";
RL Genome Res. 10:1617-1630(2000).
RN [3] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=11076861; DOI=10.1101/gr.152600;
RA Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT pipeline with 384 multicapillary sequencer.";
RL Genome Res. 10:1757-1771(2000).
RN [4] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RA Adachi J., Aizawa K., Akahira S., Akimura T., Arai A., Aono H., Arakawa T.,
RA Bono H., Carninci P., Fukuda S., Fukunishi Y., Furuno M., Hanagaki T.,
RA Hara A., Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Imotani K.,
RA Ishii Y., Itoh M., Izawa M., Kasukawa T., Kato H., Kawai J., Kojima Y.,
RA Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T., Miyazaki A.,
RA Nishi K., Nomura K., Numazaki R., Ohno M., Okazaki Y., Okido T., Owa C.,
RA Saito H., Saito R., Sakai C., Sakai K., Sano H., Sasaki D., Shibata K.,
RA Shibata Y., Shinagawa A., Shiraki T., Sogabe Y., Suzuki H., Tagami M.,
RA Tagawa A., Takahashi F., Tanaka T., Tejima Y., Toya T., Yamamura T.,
RA Yasunishi A., Yoshida K., Yoshino M., Muramatsu M., Hayashizaki Y.;
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=11217851; DOI=10.1038/35055500;
RG The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT "Functional annotation of a full-length mouse cDNA collection.";
RL Nature 409:685-690(2001).
RN [6] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=12466851; DOI=10.1038/nature01266;
RG The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT "Analysis of the mouse transcriptome based on functional annotation of
RT 60,770 full-length cDNAs.";
RL Nature 420:563-573(2002).
RN [7] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RG The FANTOM Consortium;
RG Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT "The Transcriptional Landscape of the Mammalian Genome.";
RL Science 309:1559-1563(2005).
RN [8] {ECO:0000313|EMBL:BAB30697.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB30697.1};
RC TISSUE=Head {ECO:0000313|EMBL:BAB30697.1};
RX PubMed=16141073; DOI=10.1126/science.1112009;
RG RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT "Antisense Transcription in the Mammalian Transcriptome.";
RL Science 309:1564-1566(2005).
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DR EMBL; AK017342; BAB30697.1; -; mRNA.
DR MEROPS; I02.008; -.
DR PeptideAtlas; Q9D3K4; -.
DR AGR; MGI:1338033; -.
DR MGI; MGI:1338033; Spint1.
DR ChiTaRS; Spint1; mouse.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR CDD; cd22623; Kunitz_HAI1_1-like; 1.
DR CDD; cd22624; Kunitz_HAI1_2-like; 1.
DR CDD; cd00112; LDLa; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 4.10.400.10; Low-density Lipoprotein Receptor; 1.
DR Gene3D; 4.10.410.10; Pancreatic trypsin inhibitor Kunitz domain; 2.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR036055; LDL_receptor-like_sf.
DR InterPro; IPR023415; LDLR_class-A_CS.
DR InterPro; IPR002172; LDrepeatLR_classA_rpt.
DR InterPro; IPR013980; MANSC_dom.
DR InterPro; IPR011106; MANSC_N.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR PANTHER; PTHR46750; KUNITZ-TYPE PROTEASE INHIBITOR 1; 1.
DR PANTHER; PTHR46750:SF1; KUNITZ-TYPE PROTEASE INHIBITOR 1; 1.
DR Pfam; PF00014; Kunitz_BPTI; 2.
DR Pfam; PF00057; Ldl_recept_a; 1.
DR Pfam; PF07502; MANEC; 1.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 2.
DR SMART; SM00192; LDLa; 1.
DR SMART; SM00765; MANEC; 1.
DR SUPFAM; SSF57362; BPTI-like; 2.
DR SUPFAM; SSF57424; LDL receptor-like module; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 2.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 2.
DR PROSITE; PS01209; LDLRA_1; 1.
DR PROSITE; PS50068; LDLRA_2; 1.
DR PROSITE; PS50986; MANSC; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157, ECO:0000256|PROSITE-
KW ProRule:PRU00124}; Membrane {ECO:0000256|SAM:Phobius};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..507
FT /note="Kunitz-type protease inhibitor 1"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004325062"
FT TRANSMEM 444..466
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 51..134
FT /note="MANSC"
FT /evidence="ECO:0000259|PROSITE:PS50986"
FT DOMAIN 244..294
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000259|PROSITE:PS50279"
FT DOMAIN 369..419
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000259|PROSITE:PS50279"
FT REGION 476..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 313..325
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00124"
FT DISULFID 320..338
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00124"
FT DISULFID 332..347
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00124"
SQ SEQUENCE 507 AA; 56573 MW; 9EE0A29B7056D72D CRC64;
MAGRRLARAS ISAVGVWLLC ALGLQATEAX LPSAPAELPG GAACLSRFTS GVPSFVLDTE
ASVSNGATFL GSPTARRGWD CVRSCCTTQN CNLALVELQP DGGEDAISAC FLMNCLYEQN
FVCKFAPKEG FINYLTQELY RSYRELRTRG FGGSRIPRIW MGIDLKVQLQ KPLVLNEADN
TDWHLLQGDS DVRVERKRPE EVELWGLKEG TYLFQLTRTD SDQPEETANL TITVLTAKQT
EDYCLASYKV GRCRGSFPRW YYDPKEQICK SFTFGGCLGN KNNYLREEEC MLACKDVQGI
SPKRHHPVCS GSCHATQFRC SNGCCIDGFL ECDDTPDCPD GSDEATCEKY TSGFDELQNI
HFLSDKGYCA ELPDTGFCKE NIPRWYYNPF SERCARFTYG GCYGNKNNFE EEQQCLESCR
GISKKDVFGL RREGSIPTVG SAEVAIAVFL VICIIVVLTI LGYCFFKNQR KEFHSPLHHP
PPTPASSTVS TTEDTEHLVY NHTTQPL
//
