ID MCTS1_MOUSE Reviewed; 181 AA.
AC Q9DB27; Q3UUI6;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 29-MAY-2013, entry version 96.
DE RecName: Full=Malignant T-cell-amplified sequence 1;
DE Short=MCT-1;
DE AltName: Full=Multiple copies T-cell malignancies 1;
GN Name=Mcts1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Cerebellum, Hypothalamus, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S.,
RA She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W.,
RA Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T.,
RA Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J.,
RA Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z.,
RA Lindblad-Toh K., Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of
RT the mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Anti-oncogene that play a role in cell cycle regulation;
CC decreases cell doubling time and anchorage-dependent growth;
CC shortens the duration of G1 transit time and G1/S transition. When
CC constituvely expressed, increases CDK4 and CDK6 kinases activity
CC and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK
CC complex formation. Plays a role as translation enhancer; Recruits
CC the density-regulated protein/DENR and binds to the cap complex of
CC the 5'-terminus of mRNAs, subsequently altering the mRNA
CC translation profile; Up-regulates protein levels of BCL2L2, TFDP1,
CC MRE11A, CCND1 and E2F1, while mRNA levels remains constant.
CC Hyperactivates DNA damage signaling pathway; increased gamma-
CC irradiation-induced phosphorylation of histone H2AX, and induces
CC damage foci formation. Increases the overall number of chromosomal
CC abnormalities such as larger chromosomes formation and multiples
CC chromosomal fusions when overexpressed in gamma-irradiated cells.
CC May play a role in promoting lymphoid tumor development: lymphoid
CC cell lines overexpressing MCTS1 exhibit increased growth rates and
CC display increased protection against apoptosis. May contribute to
CC the pathogenesis and progression of breast cancer via promotion of
CC angiogenesis through the decline of inhibitory
CC THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in
CC the process of proteasome degradation to down-regulate Tumor
CC suppressor p53/TP53 in breast cancer cell; Positively regulates
CC phosphorylation of MAPK1 and MAPK3 (By similarity).
CC -!- SUBUNIT: Interacts (via PUA domain) with DENR (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9DB27-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DB27-2; Sequence=VSP_034857;
CC -!- DOMAIN: The PUA RNA-binding domain is critical for cap binding,
CC but not sufficient for translation enhancer function. MCT1 N-
CC terminal region is required to enhance translation possibly
CC through interaction with other proteins (By similarity).
CC -!- PTM: Phosphorylation is critical for stabilization and promotion
CC of cell proliferation (By similarity).
CC -!- SIMILARITY: Belongs to the MCTS1 family.
CC -!- SIMILARITY: Contains 1 PUA domain.
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DR EMBL; AK005292; BAB23936.1; -; mRNA.
DR EMBL; AK087975; BAC40069.1; -; mRNA.
DR EMBL; AK138385; BAE23639.1; -; mRNA.
DR EMBL; AL513356; CAM17146.1; -; Genomic_DNA.
DR EMBL; AL845279; CAM17146.1; JOINED; Genomic_DNA.
DR EMBL; AL845279; CAM20158.1; -; Genomic_DNA.
DR EMBL; AL513356; CAM20158.1; JOINED; Genomic_DNA.
DR EMBL; BC010486; AAH10486.1; -; mRNA.
DR IPI; IPI00626860; -.
DR IPI; IPI00874436; -.
DR RefSeq; NP_081178.1; NM_026902.3.
DR UniGene; Mm.262453; -.
DR ProteinModelPortal; Q9DB27; -.
DR SMR; Q9DB27; 1-181.
DR MINT; MINT-4128072; -.
DR PhosphoSite; Q9DB27; -.
DR PaxDb; Q9DB27; -.
DR PRIDE; Q9DB27; -.
DR DNASU; 68995; -.
DR Ensembl; ENSMUST00000000365; ENSMUSP00000000365; ENSMUSG00000000355.
DR GeneID; 68995; -.
DR KEGG; mmu:68995; -.
DR UCSC; uc009tad.1; mouse.
DR UCSC; uc009tae.1; mouse.
DR CTD; 28985; -.
DR MGI; MGI:1916245; Mcts1.
DR eggNOG; COG2016; -.
DR GeneTree; ENSGT00550000074964; -.
DR HOGENOM; HOG000223988; -.
DR HOVERGEN; HBG105551; -.
DR KO; K07575; -.
DR OrthoDB; EOG4H464T; -.
DR NextBio; 328373; -.
DR Bgee; Q9DB27; -.
DR Genevestigator; Q9DB27; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:response to DNA damage stimulus; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
DR Gene3D; 2.30.130.10; -; 1.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_domain.
DR InterPro; IPR016437; Transl_RNA-bd_prd.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR22798; PTHR22798; 1.
DR Pfam; PF01472; PUA; 1.
DR PIRSF; PIRSF005067; Tma_RNA-bind_prd; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; PUA-like; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Complete proteome; Cytoplasm;
KW DNA damage; Growth regulation; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Tumor suppressor.
FT CHAIN 1 181 Malignant T-cell-amplified sequence 1.
FT /FTId=PRO_0000344787.
FT DOMAIN 92 171 PUA.
FT MOD_RES 81 81 Phosphothreonine (By similarity).
FT MOD_RES 118 118 Phosphoserine (By similarity).
FT VAR_SEQ 1 4 MFKK -> MGKGR (in isoform 2).
FT /FTId=VSP_034857.
SQ SEQUENCE 181 AA; 20555 MW; A8E02C2F992B74BD CRC64;
MFKKFDEKEN VSNCIQLKTS VIKGIKNQLL EQFPGIEPWL NQIMPKKDPV KIVRCHEHIE
ILTVNGELLF FRQREGPFYP TLRLLHKYPF ILPHQQVDKG AIKFVLSGAN IMCPGLTSPG
AKLYPAAVDT IVAIMAEGKQ HALCVGVMKM SAEDIEKVNK GIGIENIHYL NDGLWHMKTY
K
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