ID Q9FQ62_BRAOL Unreviewed; 663 AA.
AC Q9FQ62;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 24-JAN-2024, entry version 67.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
OS Brassica oleracea (Wild cabbage).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3712 {ECO:0000313|EMBL:AAG36942.1};
RN [1] {ECO:0000313|EMBL:AAG36942.1}
RP NUCLEOTIDE SEQUENCE.
RA Coupe S.A., Sinclair B.K., Greer L.A., Gapper N.E., Watson L.M.,
RA Hurst P.L.;
RT "Analysis of acid invertase gene expression during the senescence of
RT broccoli florets.";
RL Postharvest Biol. Technol. 28:27-37(2003).
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; AF274298; AAG36942.1; -; mRNA.
DR AlphaFoldDB; Q9FQ62; -.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR ExpressionAtlas; Q9FQ62; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF1; ACID BETA-FRUCTOFURANOSIDASE 4, VACUOLAR; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 40..59
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 17..116
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 124..444
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 447..643
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 663 AA; 73613 MW; 12DF13E01EB0696D CRC64;
MASSDALLPI SAREEDPLLS DGSRCDPNAE THRRRRPVKG LLAVSFGLLF IAFYVVLIAT
HDGSSRSNDV KIESDETATT TSRARLAGVS EKSNGQLWKL SGERNTVSFA WNNSVLSWQR
TAFHFQPEQN WMNDPNGPLF YKGWYHFFYQ YNPNAAVWGD IVWGHAVSKD LIHWVHLPIA
MVADQWYDAN GVWTGSATFL DDGSLVMLYT GSTDKSVQVQ NLAYPEDLND PLLLKWVKYS
GNPVLVPPPG ILPKDFRDPT TAWKTSDGKW RITIGSKINK TGISLVYDTI DFKTYEKHDT
LLHKVPNTGM WECVDFYPVS KTAVNGLDTS VNGPNVKHIV KASMDDTRFD HYAVGTYFDS
NGTWIPDDPT IDVGMSASLR YDYGKFYASK TFYDQNKGRR ILWSWIGESD SEAADVQKGW
SSLQGIPRTV VLDTKTGKNL VQWPVEEIKS LRLSSKQFDM EVGPGSVVPV DVDSAAQLDI
EAEFEINKES LDKILGDASV VVEAEEFSCQ KSGGASVRGA LGPFGFSVLA DKSLSEQTPV
YFYVAKGKDS KLKTFFCTDT SRSTFANDVV KPIYGSSVPV LKGEKLTMRI LVDHSIVEGF
AQAGRTCISS RVYPTKAIYG AAKLFVFNNA IDATITASFK VWQMNSAFIH PYSEEAVRAL
SST
//
