ID   Q9FSF9_TOBAC            Unreviewed;       715 AA.
AC   Q9FSF9;
DT   01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 91.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN   Name=TP5 {ECO:0000313|EMBL:CAC13966.1};
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097 {ECO:0000313|EMBL:CAC13966.1};
RN   [1] {ECO:0000313|EMBL:CAC13966.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=11181714; DOI=10.1093/jexbot/52.354.67;
RA   Rogers H.J., Maund S.L., Johnson L.H.;
RT   "A beta-galactosidase-like gene is expressed during tobacco pollen
RT   development.";
RL   J. Exp. Bot. 52:67-75(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412,
CC         ECO:0000256|RuleBase:RU000675};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR   EMBL; AJ250431; CAC13966.1; -; mRNA.
DR   AlphaFoldDB; Q9FSF9; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   ProMEX; Q9FSF9; -.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF206; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..715
FT                   /note="Beta-galactosidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004325585"
FT   DOMAIN          34..340
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          364..418
FT                   /note="Beta-galactosidase beta-sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF17834"
FT   DOMAIN          620..685
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
SQ   SEQUENCE   715 AA;  81316 MW;  ADD8B8CAF9D4B301 CRC64;
     MAISSRLLSL ALLSLLVSSA IGEKTKGVTY DGRSMIVNGE RELLFSGSIH YPRMPPEMWP
     DIIRKAKEGG LNLIQTYVFW NIHEPVQGQF NFEGNYDVVK FIKTIGEQGL YVTLRIGPYI
     EAEWNQGGFP YWLREVPNIT FRSYNEPFIH HMKKYSEMVI DLMKKEKLFA PQGGPIIMAQ
     IENEYNNVQL AYRDNGKKYV EWAANMATGL YNGVPWIMCK QKDAPAQVIN TCNGRHCADT
     FTGPNGPNKP SLWTENWTAQ YRTFGDPPSQ RAAEDIAFSV ARFFAKNGTL TNYYMYYGGT
     NYGRTGSSFV TTRYYDEAPL DEFGLYREPK WSHLRDLHRA LRLSRRALLW GTPSVQKINQ
     HLEITVYEKP GTDCAAFLTN NHTTLPATIK FRGREYYLPE KSVSILPDCK LLSTNTQTIV
     SQHNSRNFLP SEKAKNLKWE MYQEKVPTIS DLSLKNREPL ELYSLTKDTS DYAWYSTSIN
     FDRHDLPMRP DILPVLQIAS MGHALSAFVN GEFVGFGHGN NIEKSFVFQK PVILKPGTNT
     ISILAETVGF PNSGAYMEKR FAGPRGITVQ GLMAGTLDIT QNNWGHEVGV FGEKEQLFTE
     EGAKKVKWTP VNGPTKGAVT WYKTYFDAPE GNNPVALKMD KMQKGMMWVN GNSLGRYWSS
     FLSPLGQPTQ FEYHIPRAFL KPTNNLLVIF EETGGHPETI EVQIVNRDTN LQHHY
//