ID Q9GNP1_CIOSA Unreviewed; 770 AA.
AC Q9GNP1;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 24-JAN-2024, entry version 112.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=CsDEAD1b(CsVHb) {ECO:0000313|EMBL:BAB12217.1};
OS Ciona savignyi (Pacific transparent sea squirt).
OC Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC Cionidae; Ciona.
OX NCBI_TaxID=51511 {ECO:0000313|EMBL:BAB12217.1};
RN [1] {ECO:0000313|EMBL:BAB12217.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Ovary {ECO:0000313|EMBL:BAB12217.1};
RX PubMed=10664149; DOI=10.1007/s004270050012;
RA Fujimura M., Takamura K.;
RT "Characterization of an ascidian DEAD-box gene, Ci-DEAD1: specific
RT expression in the germ cells and its mRNA localization in the posterior-
RT most blastomeres in early embryos.";
RL Dev. Genes Evol. 210:64-72(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX4/VASA
CC subfamily. {ECO:0000256|ARBA:ARBA00010132}.
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DR EMBL; AB047803; BAB12217.1; -; mRNA.
DR AlphaFoldDB; Q9GNP1; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd18052; DEADc_DDX4; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 4.10.60.10; Zinc finger, CCHC-type; 6.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR PANTHER; PTHR47958; ATP-DEPENDENT RNA HELICASE DBP3; 1.
DR PANTHER; PTHR47958:SF11; RNA HELICASE; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00098; zf-CCHC; 6.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00343; ZnF_C2HC; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF57756; Retrovirus zinc finger-like domains; 4.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
DR PROSITE; PS50158; ZF_CCHC; 6.
PE 2: Evidence at transcript level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU000492};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU000492};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000492};
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU00047};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU000492};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU00047};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU00047}.
FT DOMAIN 108..123
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 133..148
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 160..175
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 190..205
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 215..230
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 240..255
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 313..341
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 344..527
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 555..702
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 717..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 313..341
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
SQ SEQUENCE 770 AA; 82032 MW; 5C6D2A2D8C9CDD58 CRC64;
MSDDDWDAPS SGPVSTGFGG GSSFGETISK GGGRGRGKFA DRGDYNQPND YGFGSGFGKS
DDGGFGSKPN SGFGKSNFDD DTGFGGGFGS SSGGGFGDTR GSSRSKGCFK CGEEGHMSRE
CPQGGGGSRG KGCFKCGEEG HMSRECPKGG GGGGGGGRGC FKCGEEGHMS RECPKGGDSG
FEGRSRSKGC FKCGEEGHMS RECPQGGGGG RGSGCFKCGE EGHMSRECPQ GGGGGRGSGC
FKCGEEGHMS RECPRNTSGE GGEKSDRPPI YIPPPPPEDE VEMFASMQRG INFGKYDAIP
VEVSGVNAPK SIPTFEVAGL PETVLANVKR ANYERPTPVQ KYSIPIINAD RDLMACAQTG
SGKTAAFLLP VLTKLITNGL QSSQFSEKQT PRAIVVGPTR ELIYQIFLEA RKFSRGTVVR
PVVAYGGTSM NHQIRDLQRG CHILIATPGR LMDFINRGLV GLDHVEFVIL DEADRMLDMG
FETEIRKLAS SPGMPSKSDR HTLMFSATFP DEIQRLAHDF LREDFLFLTV GRVGGACTDV
TQSIIQVDQD DKRAKLLELI SDVAETRSRT LVFVETKRGA DFLACMLSQE GCPTTSIHGD
RLQQEREQAL RDFKSAVCPI LIATSVAARG LDIPKVEHVI NYDMPKEIDE YVHRIGRTGR
CGNLGRATTF YDNNKDGELA RSLVKILSEA QQEVPGWLEE CAESAVGSSF GKEGGFGGRD
FRKRGGRNDT GFGGSKGGFG SRSTADCDYN DGGGFGNTAA VSKDDDDSWD
//
