ID IL22_HUMAN Reviewed; 179 AA.
AC Q9GZX6;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 01-MAY-2013, entry version 121.
DE RecName: Full=Interleukin-22;
DE Short=IL-22;
DE AltName: Full=Cytokine Zcyto18;
DE AltName: Full=IL-10-related T-cell-derived-inducible factor;
DE Short=IL-TIF;
DE Flags: Precursor;
GN Name=IL22; Synonyms=ILTIF, ZCYTO18; ORFNames=UNQ3099/PRO10096;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10954742; DOI=10.1073/pnas.170291697;
RA Dumoutier L., Van Roost E., Colau D., Renauld J.-C.;
RT "Human interleukin-10-related T cell-derived inducible factor:
RT molecular cloning and functional characterization as an hepatocyte-
RT stimulating factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10144-10149(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11197690; DOI=10.1038/sj.gene.6363716;
RA Dumoutier L., Van Roost E., Colau D., Ameye G., Michaux L.,
RA Renauld J.-C.;
RT "IL-TIF/IL-22: genomic organization and mapping of the human and mouse
RT genes.";
RL Genes Immun. 1:488-494(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10875937; DOI=10.1074/jbc.M005304200;
RA Xie M.-H., Aggarwal S., Ho W.-H., Foster J., Zhang Z., Stinson J.,
RA Wood W.I., Goddard A.D., Gurney A.L.;
RT "Interleukin (IL)-22, a novel human cytokine that signals through the
RT interferon receptor-related proteins CRF2-4 and IL-22R.";
RL J. Biol. Chem. 275:31335-31339(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT GLY-158.
RG SeattleSNPs variation discovery resource;
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Blood;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 34-48.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally
RT verified cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 29-179.
RX PubMed=11856845; DOI=10.1107/S0907444902001063;
RA Nagem R.A., Lucchesi K.W., Colau D., Dumoutier L., Renauld J.-C.,
RA Polikarpov I.;
RT "Crystallization and synchrotron X-ray diffraction studies of human
RT interleukin-22.";
RL Acta Crystallogr. D 58:529-530(2002).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 38-179, AND GLYCOSYLATION AT
RP ASN-54 AND ASN-97.
RX PubMed=15983417; DOI=10.1107/S0907444905009601;
RA Xu T., Logsdon N.J., Walter M.R.;
RT "Structure of insect-cell-derived IL-22.";
RL Acta Crystallogr. D 61:942-950(2005).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 39-179 IN COMPLEX WITH
RP IL22RA1, AND DISULFIDE BONDS.
RX PubMed=18599299; DOI=10.1016/j.str.2008.06.005;
RA Jones B.C., Logsdon N.J., Walter M.R.;
RT "Structure of IL-22 bound to its high-affinity IL-22R1 chain.";
RL Structure 16:1333-1344(2008).
CC -!- FUNCTION: Cytokine that contributes to the inflammatory response
CC in vivo.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the IL-10 family.
CC -!- WEB RESOURCE: Name=SeattleSNPs;
CC URL="http://pga.gs.washington.edu/data/il22/";
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DR EMBL; AJ277247; CAC06085.1; -; mRNA.
DR EMBL; AJ277248; CAC19409.1; -; Genomic_DNA.
DR EMBL; AF279437; AAG22064.1; -; mRNA.
DR EMBL; AY358890; AAQ89249.1; -; mRNA.
DR EMBL; AF387519; AAK62468.1; -; Genomic_DNA.
DR EMBL; BC066263; AAH66263.1; -; mRNA.
DR EMBL; BC067510; AAH67510.1; -; mRNA.
DR EMBL; BC069112; AAH69112.1; -; mRNA.
DR EMBL; BC069308; AAH69308.1; -; mRNA.
DR EMBL; BC070261; AAH70261.1; -; mRNA.
DR IPI; IPI00008372; -.
DR RefSeq; NP_065386.1; NM_020525.4.
DR UniGene; Hs.287369; -.
DR PDB; 1M4R; X-ray; 2.00 A; A/B=29-179.
DR PDB; 1YKB; X-ray; 2.60 A; A/B/C/D/E/F=38-179.
DR PDB; 3DGC; X-ray; 2.50 A; L/M=39-179.
DR PDB; 3DLQ; X-ray; 1.90 A; I=29-179.
DR PDB; 3G9V; X-ray; 2.76 A; B/D=29-179.
DR PDB; 3Q1S; X-ray; 2.15 A; I=29-179.
DR PDBsum; 1M4R; -.
DR PDBsum; 1YKB; -.
DR PDBsum; 3DGC; -.
DR PDBsum; 3DLQ; -.
DR PDBsum; 3G9V; -.
DR PDBsum; 3Q1S; -.
DR ProteinModelPortal; Q9GZX6; -.
DR DIP; DIP-46035N; -.
DR MINT; MINT-6693924; -.
DR STRING; 9606.ENSP00000329384; -.
DR DMDM; 17366814; -.
DR PaxDb; Q9GZX6; -.
DR PRIDE; Q9GZX6; -.
DR DNASU; 50616; -.
DR Ensembl; ENST00000328087; ENSP00000329384; ENSG00000127318.
DR Ensembl; ENST00000538666; ENSP00000442424; ENSG00000127318.
DR GeneID; 50616; -.
DR KEGG; hsa:50616; -.
DR UCSC; uc001sty.1; human.
DR CTD; 50616; -.
DR GeneCards; GC12M068642; -.
DR HGNC; HGNC:14900; IL22.
DR MIM; 605330; gene.
DR neXtProt; NX_Q9GZX6; -.
DR PharmGKB; PA29822; -.
DR eggNOG; NOG42949; -.
DR HOGENOM; HOG000280686; -.
DR HOVERGEN; HBG018572; -.
DR InParanoid; Q9GZX6; -.
DR KO; K05445; -.
DR OMA; LDLLFMA; -.
DR OrthoDB; EOG4DJJXR; -.
DR PhylomeDB; Q9GZX6; -.
DR EvolutionaryTrace; Q9GZX6; -.
DR GenomeRNAi; 50616; -.
DR NextBio; 53128; -.
DR Bgee; Q9GZX6; -.
DR CleanEx; HS_IL22; -.
DR Genevestigator; Q9GZX6; -.
DR GermOnline; ENSG00000127318; Homo sapiens.
DR GO; GO:0005576; C:extracellular region; IC:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0045518; F:interleukin-22 receptor binding; NAS:UniProtKB.
DR GO; GO:0006953; P:acute-phase response; NAS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; IC:UniProtKB.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR012351; 4_helix_cytokine_core.
DR InterPro; IPR020423; Interleukin-10_CS.
DR InterPro; IPR020453; Interleukin-22.
DR PIRSF; PIRSF037726; Interleukin-22; 1.
DR PRINTS; PR01936; INTRLEUKIN22.
DR SUPFAM; SSF47266; 4_helix_cytokine; 1.
DR PROSITE; PS00520; INTERLEUKIN_10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytokine; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Polymorphism; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1 33
FT CHAIN 34 179 Interleukin-22.
FT /FTId=PRO_0000015383.
FT CARBOHYD 54 54 N-linked (GlcNAc...).
FT CARBOHYD 68 68 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 97 97 N-linked (GlcNAc...).
FT DISULFID 40 132
FT DISULFID 89 178
FT VARIANT 158 158 S -> G (in dbSNP:rs2227507).
FT /FTId=VAR_013078.
FT HELIX 44 47
FT HELIX 50 65
FT STRAND 74 76
FT HELIX 77 80
FT HELIX 85 87
FT HELIX 88 102
FT TURN 103 109
FT HELIX 114 129
FT HELIX 141 155
FT HELIX 157 165
FT HELIX 167 178
SQ SEQUENCE 179 AA; 20011 MW; 3C35E64D60CF8767 CRC64;
MAALQKSVSS FLMGTLATSC LLLLALLVQG GAAAPISSHC RLDKSNFQQP YITNRTFMLA
KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP
FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI
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