ID RTCA_PSEAE Reviewed; 341 AA.
AC Q9HVJ9;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 01-MAY-2013, entry version 76.
DE RecName: Full=RNA 3'-terminal phosphate cyclase;
DE Short=RNA cyclase;
DE Short=RNA-3'-phosphate cyclase;
DE EC=6.5.1.4;
GN Name=rtcA; OrderedLocusNames=PA4585;
OS Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG
OS 12228).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Catalyzes the conversion of 3'-phosphate to a 2',3'-
CC cyclic phosphodiester at the end of RNA. The mechanism of action
CC of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by
CC ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-
CC N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-
CC phosphorus in the diester linkage to produce the cyclic end
CC product. The biological role of this enzyme is unknown but it is
CC likely to function in some aspects of cellular RNA processing (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + RNA 3'-terminal-phosphate = AMP +
CC diphosphate + RNA terminal-2',3'-cyclic-phosphate.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the RNA 3'-terminal cyclase family. Type 1
CC subfamily.
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DR EMBL; AE004091; AAG07973.1; -; Genomic_DNA.
DR PIR; E83072; E83072.
DR RefSeq; NP_253275.1; NC_002516.2.
DR ProteinModelPortal; Q9HVJ9; -.
DR SMR; Q9HVJ9; 3-331.
DR STRING; 208964.PA4585; -.
DR GeneID; 881010; -.
DR KEGG; pae:PA4585; -.
DR PATRIC; 19843915; VBIPseAer58763_4799.
DR PseudoCAP; PA4585; -.
DR eggNOG; COG0430; -.
DR HOGENOM; HOG000015264; -.
DR KO; K01974; -.
DR OMA; RRGHYPK; -.
DR ProtClustDB; PRK04204; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003963; F:RNA-3'-phosphate cyclase activity; IEA:HAMAP.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.30.360.20; -; 1.
DR Gene3D; 3.65.10.20; -; 2.
DR HAMAP; MF_00200; RTC; 1; -.
DR InterPro; IPR013791; RNA3'-term_phos_cycl_insert.
DR InterPro; IPR023797; RNA3'_phos_cyclase_dom.
DR InterPro; IPR000228; RNA3'_term_phos_cyc.
DR InterPro; IPR017770; RNA3'_term_phos_cyc_type_1.
DR InterPro; IPR020719; RNA3'_term_phos_cycl-like_CS.
DR InterPro; IPR013792; RNA3'P_cycl/enolpyr_Trfase_a/b.
DR PANTHER; PTHR11096; PTHR11096; 1.
DR Pfam; PF01137; RTC; 1.
DR Pfam; PF05189; RTC_insert; 1.
DR SUPFAM; SSF52913; RNA3'-term_phos_cycl_insert; 1.
DR SUPFAM; SSF55205; RNA3'_cycl/enolpyr_transf_A/B; 2.
DR TIGRFAMs; TIGR03399; RNA_3prim_cycl; 1.
DR PROSITE; PS01287; RTC; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1 341 RNA 3'-terminal phosphate cyclase.
FT /FTId=PRO_0000156419.
FT NP_BIND 283 287 ATP (By similarity).
FT ACT_SITE 308 308 Tele-AMP-histidine intermediate (By
FT similarity).
FT BINDING 102 102 ATP (By similarity).
SQ SEQUENCE 341 AA; 36637 MW; E7277E130981D24F CRC64;
MRKDLIELDG SEGGGQILRS ALSLSMTSGQ PLRIRNIRGR RSRPGLLRQH LTAVRAAAEI
CAAEVEGAEL GSRELAFRPG AIRAGDYAFA IGSAGSCSLV LQTLLPALLA ANGESRVRIS
GGTHNPLAPP ADFLRDSWLP LLQRMGAEVD LELLRHGFVP AGGGELLARV RPARWRPLQL
EHPGAALRRQ ARALLAGIPG HVGERELERV RQRLGWSDEE RQLEFLAEDQ GPGNALLLRI
DCEHICATFC AFGQAGVSAE RVAEQVATQA IGWMESGCAA DEHLADQLLL PMALAGAGSF
TTPRLSAHLQ SNRRVIERFL PVRIGDQALD GGGHRIVITS A
//
