ID RLUB_PSEAE Reviewed; 386 AA.
AC Q9HZ55;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 01-MAY-2013, entry version 67.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase B;
DE EC=5.4.99.22;
DE AltName: Full=23S rRNA pseudouridine(2605) synthase;
DE AltName: Full=rRNA pseudouridylate synthase B;
DE AltName: Full=rRNA-uridine isomerase B;
GN Name=rluB; OrderedLocusNames=PA3179;
OS Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG
OS 12228).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 2605 in 23S ribosomal RNA (By similarity).
CC -!- CATALYTIC ACTIVITY: 23S rRNA uridine(2605) = 23S rRNA
CC pseudouridine(2605).
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC -!- SIMILARITY: Contains 1 S4 RNA-binding domain.
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DR EMBL; AE004091; AAG06567.1; -; Genomic_DNA.
DR PIR; E83247; E83247.
DR RefSeq; NP_251869.1; NC_002516.2.
DR ProteinModelPortal; Q9HZ55; -.
DR SMR; Q9HZ55; 15-247.
DR STRING; 208964.PA3179; -.
DR GeneID; 882705; -.
DR KEGG; pae:PA3179; -.
DR PATRIC; 19840925; VBIPseAer58763_3323.
DR PseudoCAP; PA3179; -.
DR eggNOG; COG1187; -.
DR HOGENOM; HOG000044954; -.
DR KO; K06178; -.
DR OMA; PYAPRDG; -.
DR ProtClustDB; CLSK867796; -.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluD.
DR InterPro; IPR002942; S4_RNA-bd.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; Reference proteome; RNA-binding;
KW rRNA processing.
FT CHAIN 1 386 Ribosomal large subunit pseudouridine
FT synthase B.
FT /FTId=PRO_0000099988.
FT DOMAIN 14 75 S4 RNA-binding.
FT ACT_SITE 119 119 Nucleophile (By similarity).
SQ SEQUENCE 386 AA; 43725 MW; C47840D7C0E5C0CC CRC64;
MNDTPENPHV PVGEKLQKVL ARLGVGSRRD VEVWIAEGRV NVNGSVASLG QRVDSHDAIT
VDGHLIRREE AAESVRRVLI YNKPEGEVCT RDDPEGRPTI FDRLPRLRTG RWINVGRLDI
NTTGLLLFTT DGELANRLMH PSYEMDREYA VRVRGEVTEE MIERLLNGVM LEDGPAKFSD
IQQAPGGEGF NHWYHCVVME GRNREVRRLW ESQGLVVSRL KRVRFGPVFL TSELTMGRYR
EMDQREIDIL SEEVGLKPVA LPGMTTKARE KAERQQRKQA RPLARSERPE AGRKRAPRRE
DGENAARRAP ASRPARGPQP SAERKGREQG TPVAERPRES NRKPRPSKPR DERPASAPGD
KPAARKPQVK RRPKPAGDGM RPGFRR
//
