ID DNAA_PSEAE Reviewed; 514 AA.
AC Q9I7C5;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 131.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; OrderedLocusNames=PA0001;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO116;
RX PubMed=10749858; DOI=10.1074/jbc.m000552200;
RA Caspi R., Helinski D.R., Pacek M., Konieczny I.;
RT "Interactions of DnaA proteins from distantly related bacteria with the
RT replication origin of the broad host range plasmid RK2.";
RL J. Biol. Chem. 275:18454-18461(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
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DR EMBL; AF229442; AAG28797.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG03391.1; -; Genomic_DNA.
DR PIR; E83644; E83644.
DR RefSeq; NP_064721.1; NC_002516.2.
DR RefSeq; WP_003109151.1; NZ_QZGE01000012.1.
DR AlphaFoldDB; Q9I7C5; -.
DR SMR; Q9I7C5; -.
DR STRING; 208964.PA0001; -.
DR PaxDb; 208964-PA0001; -.
DR GeneID; 878417; -.
DR KEGG; pae:PA0001; -.
DR PATRIC; fig|208964.12.peg.1; -.
DR PseudoCAP; PA0001; -.
DR HOGENOM; CLU_026910_0_1_6; -.
DR InParanoid; Q9I7C5; -.
DR OrthoDB; 9807019at2; -.
DR PhylomeDB; Q9I7C5; -.
DR BioCyc; PAER208964:G1FZ6-1-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR NCBIfam; TIGR00362; DnaA; 1.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF2; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48295; TrpR-like; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA replication; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..514
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000114238"
FT BINDING 219..226
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 514 AA; 57754 MW; ACEE3916221E2E84 CRC64;
MSVELWQQCV DLLRDELPSQ QFNTWIRPLQ VEAEGDELRV YAPNRFVLDW VNEKYLGRLL
ELLGERGEGQ LPALSLLIGS KRSRTPRAAI VPSQTHVAPP PPVAPPPAPV QPVSAAPVVV
PREELPPVTT APSVSSDPYE PEEPSIDPLA AAMPAGAAPA VRTERNVQVE GALKHTSYLN
RTFTFENFVE GKSNQLARAA AWQVADNLKH GYNPLFLYGG VGLGKTHLMH AVGNHLLKKN
PNAKVVYLHS ERFVADMVKA LQLNAINEFK RFYRSVDALL IDDIQFFARK ERSQEEFFHT
FNALLEGGQQ VILTSDRYPK EIEGLEERLK SRFGWGLTVA VEPPELETRV AILMKKAEQA
KIELPHDAAF FIAQRIRSNV RELEGALKRV IAHSHFMGRP ITIELIRESL KDLLALQDKL
VSIDNIQRTV AEYYKIKISD LLSKRRSRSV ARPRQVAMAL SKELTNHSLP EIGVAFGGRD
HTTVLHACRK IAQLRESDAD IREDYKNLLR TLTT
//
