ID Q9LQM5_ARATH Unreviewed; 688 AA.
AC Q9LQM5;
DT 01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 180.
DE SubName: Full=F5D14.10 protein {ECO:0000313|EMBL:AAF81330.1};
DE SubName: Full=NDR1/HIN1-like 8 {ECO:0000313|EMBL:AEE31466.1};
DE SubName: Full=RING finger protein, putative {ECO:0000313|EMBL:AAM20400.1};
GN Name=NHL8 {ECO:0000313|EMBL:AEE31466.1, ECO:0000313|TAIR:AT1G32340};
GN Synonyms=F5D14.10 {ECO:0000313|EMBL:AAF81330.1}, NDR1/HIN1-like 8
GN {ECO:0000313|EMBL:AEE31466.1};
GN OrderedLocusNames=At1g32340 {ECO:0000313|Araport:AT1G32340,
GN ECO:0000313|EMBL:AAM20400.1};
GN ORFNames=F27G20.5 {ECO:0000313|EMBL:AAG60174.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:AAF81330.1};
RN [1] {ECO:0000313|EMBL:AEE31466.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2] {ECO:0000313|EMBL:AAF81330.1}
RP NUCLEOTIDE SEQUENCE.
RA Liu S.X., Chan A., Yu G., Lee J.M., Lenz C., Pham P., Sakano H.,
RA Toriumi M., Vysotskaia V.S., Chin C., Chiou J., Choi E., Chung M.,
RA Gonzalez A., Howng B., Liu A., Vaysberg M., Altafi H., Brooks S.,
RA Buehler E., Chao Q., Conn L., Conway A.B., Hansen N.F., Johnson-Hopson C.,
RA Khan S., Kim C., Lam B., Miranda M., Nguyen M., Palm C.J., Shinn P.,
RA Southwick A., Davis R.W., Ecker J.R., Federspiel N.A., Theologis A.;
RT "The sequence of BAC F5D14 from Arabidopsis thaliana chromosome 1.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AAF81330.1}
RP NUCLEOTIDE SEQUENCE.
RA Theologis;
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAG60174.1}
RP NUCLEOTIDE SEQUENCE.
RA Lin X., Kaul S., Town C.D., Benito M., Creasy T.H., Haas B.J., Wu D.,
RA Maiti R., Ronning C.M., Koo H., Fujii C.Y., Utterback T.R., Barnstead M.E.,
RA Bowman C.L., White O., Nierman W.C., Fraser C.M.;
RT "Arabidopsis thaliana chromosome 1 BAC F27G20 genomic sequence.";
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AAM20400.1}
RP NUCLEOTIDE SEQUENCE.
RA Nguyen M., Karlin-Neumann G., Southwick A., Lam B., Miranda M., Palm C.J.,
RA Bowser L., Jones T., Banh J., Carninci P., Chen H., Cheuk R., Chung M.K.,
RA Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Kim C., Lin J., Liu S.X.,
RA Narusaka M., Pham P.K., Sakano H., Sakurai T., Satou M., Seki M., Shinn P.,
RA Yamada K., Shinozaki K., Ecker J., Theologis A., Davis R.W.;
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:AAN72126.1}
RP NUCLEOTIDE SEQUENCE.
RA Nguyen M., Karlin-Neumann G., Southwick A., Tripp M., Miranda M.,
RA Palm C.J., Bowser L., Jones T., Banh J., Carninci P., Chen H., Cheuk R.,
RA Chung M.K., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Kim C., Lin J.,
RA Liu S.X., Narusaka M., Pham P.K., Sakano H., Sakurai T., Satou M., Seki M.,
RA Shinn P., Yamada K., Shinozaki K., Ecker J., Theologis A., Davis R.W.;
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:AEE31466.1}
RP NUCLEOTIDE SEQUENCE.
RG TAIR;
RA Swarbreck D., Lamesch P., Wilks C., Huala E.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:AEE31466.1}
RP NUCLEOTIDE SEQUENCE.
RA Krishnakumar V., Cheng C.-Y., Chan A.P., Schobel S., Kim M., Ferlanti E.S.,
RA Belyaeva I., Rosen B.D., Micklem G., Miller J.R., Vaughn M., Town C.D.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR EMBL; AC007767; AAF81330.1; -; Genomic_DNA.
DR EMBL; AC084110; AAG60174.1; -; Genomic_DNA.
DR EMBL; AY099548; AAM20400.1; -; mRNA.
DR EMBL; BT002115; AAN72126.1; -; mRNA.
DR EMBL; CP002684; AEE31466.1; -; Genomic_DNA.
DR PIR; B86448; B86448.
DR RefSeq; NP_174512.1; NM_102967.3.
DR AlphaFoldDB; Q9LQM5; -.
DR STRING; 3702.Q9LQM5; -.
DR PaxDb; 3702-AT1G32340-1; -.
DR ProteomicsDB; 189496; -.
DR EnsemblPlants; AT1G32340.1; AT1G32340.1; AT1G32340.
DR GeneID; 840126; -.
DR Gramene; AT1G32340.1; AT1G32340.1; AT1G32340.
DR KEGG; ath:AT1G32340; -.
DR Araport; AT1G32340; -.
DR TAIR; AT1G32340; NHL8.
DR eggNOG; KOG1814; Eukaryota.
DR HOGENOM; CLU_021364_1_1_1; -.
DR OMA; RCFQIHI; -.
DR OrthoDB; 4338126at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQM5; baseline and differential.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd23134; RING-HC_ITT1-like; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685:SF237; E3 UBIQUITIN-PROTEIN LIGASE RNF14; 1.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 3.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF57850; RING/U-box; 4.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q9LQM5,
KW ECO:0007829|ProteomicsDB:Q9LQM5};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 191..323
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 374..601
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 378..426
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..20
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 688 AA; 78597 MW; 0E93058369C20086 CRC64;
MRGTHGGSGR NHDRRYIRRD AGIVNPVNDH PKSEHQKEDT TSTDLSSAAA FPSTSQPIQN
LDHSTKPSKS HRNRRSRGSN SKPRPVEKPE VNFAESDVVD CLADELSRLK VKQNSHSVNM
EEKFHHSSSD HSNCEESELK RAESEEIADG VDEYETKEDI MLTILKDLRS SVSEPELTEE
QLKMNDQLQE DELLALGYIY GGNMFIFDRY NDMRYFQVHV NVEATSEYTI STKLNLQADS
SKESEDFLYS FKAQHLPPIV LKCLLPKAYP SHLPPYFLIS VQWMNPDKIS SLCSKLDSLW
SEQPGQEVLY QWTDWLQNSS ISHLGFDNEI VLGPYGVTCS RDKRAVSGSR SPDSDIPYIR
SYDDEKRHDS FLQSLHECCI CFSESAGIDF VKLPCQHFFC LKCMKTYTDI HVTEGTVNKL
KCPDSKCGET VPPGILKRLL GDEAYERWET LMLQKTLESM TDVAYCPRCE TPCIEDEEQL
ALCFKCYFSF CTLCKEKRHV GVACMSPELR LQILQERQDS SRLGEEQRRK EKEMINEIMS
VKVIMKSAKQ CPSCKIAISR TGGCNKMVCN NCGQYFCYRC NEAITGYEHF SEGKCELFPQ
EAIQEWNEMM NERQVIGQIQ AQLFAQGGQF PQRGQLCPNC RQFNGKAGNN NHLFCWACQA
HFCYLCKKVV KKSAQHYGPK GCKQHTDG
//
