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Database: UniProt
Entry: Q9LWB5
LinkDB: Q9LWB5
Original site: Q9LWB5 
ID   RK23_SPIOL              Reviewed;         198 AA.
AC   Q9LWB5; Q9T2N4;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   19-FEB-2014, entry version 65.
DE   RecName: Full=50S ribosomal protein L23, chloroplastic;
DE   AltName: Full=PRPL23;
DE   Flags: Precursor;
GN   Name=RPL23;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; Caryophyllales; Amaranthaceae; Chenopodioideae;
OC   Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Matador;
RA   Jayabaskaran C., Subramanian A.R.;
RT   "Nucleotide sequence of the cDNA coding for chloroplast ribosomal
RT   protein L23 of spinach.";
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 77-113; 122-136 AND 156-193, IDENTIFICATION AS A
RP   SUBUNIT OF THE CHLOROPLAST RIBOSOME, AND DEMONSTRATION OF TWO FORMS.
RC   STRAIN=cv. Alwaro; TISSUE=Leaf;
RX   PubMed=8021938; DOI=10.1006/jmbi.1994.1415;
RA   Bubunenko M.G., Schmidt J., Subramanian A.R.;
RT   "Protein substitution in chloroplast ribosome evolution. A eukaryotic
RT   cytosolic protein has replaced its organelle homologue (L23) in
RT   spinach.";
RL   J. Mol. Biol. 240:28-41(1994).
RN   [3]
RP   PROTEIN SEQUENCE OF 77-82, AND MASS SPECTROMETRY.
RC   STRAIN=cv. Alwaro; TISSUE=Leaf;
RX   PubMed=10874046; DOI=10.1074/jbc.M005012200;
RA   Yamaguchi K., Subramanian A.R.;
RT   "The plastid ribosomal proteins. Identification of all the proteins in
RT   the 50S subunit of an organelle ribosome (chloroplast).";
RL   J. Biol. Chem. 275:28466-28482(2000).
RN   [4]
RP   STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX   PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA   Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F.,
RA   Fucini P., Agrawal R.K.;
RT   "Cryo-EM study of the spinach chloroplast ribosome reveals the
RT   structural and functional roles of plastid-specific ribosomal
RT   proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
CC   -!- FUNCTION: Binds to 23S rRNA (By similarity). Located at the
CC       polypeptide exit tunnel on the outside of the subunit.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MASS SPECTROMETRY: Mass=13553.5; Method=Electrospray; Range=77-
CC       198; Source=PubMed:10874046;
CC   -!- MISCELLANEOUS: This protein is the 80S cytosolic ortholog and is
CC       imported into the plastid, unlike the case in most plastids where
CC       it is the bacterial ortholog and is encoded in the plastid. Two
CC       forms of the protein exist; they have the same N-terminal sequence
CC       and approximately the same molecular weight.
CC   -!- SIMILARITY: Belongs to the ribosomal protein L23P family.
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DR   EMBL; X90414; CAA62040.1; -; mRNA.
DR   PDB; 3BBO; EM; 9.40 A; V=1-198.
DR   PDBsum; 3BBO; -.
DR   ProteinModelPortal; Q9LWB5; -.
DR   SMR; Q9LWB5; 110-194.
DR   EvolutionaryTrace; Q9LWB5; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:InterPro.
DR   Gene3D; 3.30.70.330; -; 1.
DR   HAMAP; MF_01369_A; Ribosomal_L23_A; 1.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait.
DR   InterPro; IPR012678; Ribosomal_L23/L15e_core_dom.
DR   InterPro; IPR013025; Ribosomal_L25/23.
DR   Pfam; PF00276; Ribosomal_L23; 1.
DR   SUPFAM; SSF54189; SSF54189; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW   Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding;
KW   Transit peptide.
FT   TRANSIT       1     76       Chloroplast.
FT   CHAIN        77    198       50S ribosomal protein L23, chloroplastic.
FT                                /FTId=PRO_5000146571.
FT   CONFLICT     83     83       P -> L (in Ref. 2; AA sequence).
FT   CONFLICT     93     93       P -> N (in Ref. 2; AA sequence).
FT   CONFLICT    101    101       K -> I (in Ref. 2; AA sequence).
FT   CONFLICT    108    108       I -> V (in Ref. 2; AA sequence).
FT   CONFLICT    171    171       T -> TE (in Ref. 2; AA sequence).
FT   CONFLICT    178    178       L -> I (in Ref. 2; AA sequence).
FT   CONFLICT    189    193       KKIGI -> TEASK (in Ref. 2; AA sequence).
SQ   SEQUENCE   198 AA;  21800 MW;  624DF1D4032BB70F CRC64;
     MATTAPNLHS LSSSFAFSNP SSNVSATSFT FQIPNKKAQI SCISSKKLHT QKSFNFHDAV
     TPMNKPSFGR DLMVAQATEA VAPTTEEAAT SQPKTSKKAK KLKYPRRILD VYQILQSPII
     TEAAIKNIAD ENSLLFTVDV RADKKMIREA ISNFFGVKVR KVNTLIRPDG TKKAYIMLNK
     EYNASELAKK IGIFPGGN
//
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