ID TPP2_ARATH Reviewed; 367 AA.
AC Q9M9Z2; Q8LEC9;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 01-MAY-2013, entry version 91.
DE RecName: Full=Probable thylakoidal processing peptidase 2, chloroplastic;
DE EC=3.4.21.89;
DE AltName: Full=Signal peptidase I-2;
DE Flags: Precursor;
GN Name=TPP2; OrderedLocusNames=At1g06870; ORFNames=F4H5.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RG The Arabidopsis Information Resource (TAIR);
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cleaves the thylakoid-transfer domain from a chloroplast
CC protein (By similarity).
CC -!- CATALYTIC ACTIVITY: Cleavage of hydrophobic, N-terminal signal or
CC leader sequences from secreted and periplasmic proteins.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane;
CC Single-pass membrane protein. Note=located in the non-appressed
CC lamellae of the thylakoid network (By similarity).
CC -!- SIMILARITY: Belongs to the peptidase S26 family.
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DR EMBL; AC011001; AAF63136.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28047.1; -; Genomic_DNA.
DR EMBL; AY085489; AAM62715.1; -; mRNA.
DR EMBL; BT024745; ABD59083.1; -; mRNA.
DR IPI; IPI00517366; -.
DR PIR; E86203; E86203.
DR RefSeq; NP_172171.1; NM_100563.3.
DR UniGene; At.23784; -.
DR HSSP; P00803; 1KN9.
DR ProteinModelPortal; Q9M9Z2; -.
DR SMR; Q9M9Z2; 203-352.
DR MEROPS; S26.A01; -.
DR PRIDE; Q9M9Z2; -.
DR EnsemblPlants; AT1G06870.1; AT1G06870.1; AT1G06870.
DR GeneID; 837198; -.
DR KEGG; ath:AT1G06870; -.
DR GeneFarm; 2115; 205.
DR TAIR; At1g06870; -.
DR eggNOG; COG0681; -.
DR HOGENOM; HOG000243179; -.
DR InParanoid; Q9M9Z2; -.
DR KO; K03100; -.
DR OMA; MAIRVTF; -.
DR PhylomeDB; Q9M9Z2; -.
DR ProtClustDB; CLSN2682520; -.
DR ChEMBL; CHEMBL1932907; -.
DR Genevestigator; Q9M9Z2; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.10.109.10; -; 2.
DR InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR InterPro; IPR015927; Peptidase_S24_S26A/B/C.
DR InterPro; IPR011056; Peptidase_S24_S26A/B/C_b-rbn.
DR InterPro; IPR019533; Peptidase_S26.
DR PANTHER; PTHR12383; PTHR12383; 1.
DR Pfam; PF10502; Peptidase_S26; 1.
DR PRINTS; PR00727; LEADERPTASE.
DR SUPFAM; SSF51306; Pept_S24_S26_C; 1.
DR TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR PROSITE; PS00501; SPASE_I_1; 1.
DR PROSITE; PS00761; SPASE_I_3; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Complete proteome; Hydrolase; Membrane; Plastid;
KW Protease; Reference proteome; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1 68 Chloroplast (Potential).
FT TRANSIT 69 ? Thylakoid (Potential).
FT CHAIN ? 367 Probable thylakoidal processing peptidase
FT 2, chloroplastic.
FT /FTId=PRO_0000310735.
FT TRANSMEM 185 205 Helical; (Potential).
FT TOPO_DOM 206 367 Lumenal, thylakoid (Potential).
FT ACT_SITE 214 214 By similarity.
FT CONFLICT 111 111 M -> V (in Ref. 3; AAM62715).
FT CONFLICT 196 196 V -> F (in Ref. 3; AAM62715).
SQ SEQUENCE 367 AA; 40150 MW; BE18813DE031BBAE CRC64;
MAIRVTFTYS SYVARSIASS AGTRVGTGDV RSCFETWVRP RFCGHNQIPD IVDKSPGSNT
WGPSSGPRAR PASSMYSTIA REILEEGCKS PLVLGMISLM NLTGAPQFSG MTGLGISPFK
TSSVIPFLRG SKWMPCSIPA TLSTDIAEVD RGGKVCDPKV KLELSDKVSN GGNGWVNKLL
NICSEDAKAA FTAVTVSLLF RSALAEPKSI PSTSMLPTLD VGDRVIAEKV SYFFRKPEVS
DIVIFKAPPI LVEHGYSCAD VFIKRIVASE GDWVEVCDGK LLVNDTVQAE DFVLEPIDYE
MEPMFVPEGY VFVLGDNRNK SFDSHNWGPL PIKNIIGRSV FRYWPPSKVS DIIHHEQVSQ
KRAVDVS
//
