ID   Q9NT06_HUMAN            Unreviewed;       157 AA.
AC   Q9NT06;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 124.
DE   RecName: Full=hydroxymethylglutaryl-CoA lyase {ECO:0000256|ARBA:ARBA00012910};
DE            EC=4.1.3.4 {ECO:0000256|ARBA:ARBA00012910};
DE   Flags: Fragment;
GN   Name=DKFZp434G1411 {ECO:0000313|EMBL:CAB70838.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:CAB70838.1};
RN   [1] {ECO:0000313|EMBL:CAB70838.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Testis {ECO:0000313|EMBL:CAB70838.1};
RA   Duesterhoeft A., Lauber J., Mewes H.W., Gassenhuber J., Wiemann S.;
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA;
CC         Xref=Rhea:RHEA:24404, ChEBI:CHEBI:13705, ChEBI:CHEBI:43074,
CC         ChEBI:CHEBI:57288; EC=4.1.3.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00001651};
CC   -!- PATHWAY: Metabolic intermediate metabolism; (S)-3-hydroxy-3-
CC       methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-
CC       methylglutaryl-CoA: step 1/1. {ECO:0000256|ARBA:ARBA00005143}.
CC   -!- SIMILARITY: Belongs to the HMG-CoA lyase family.
CC       {ECO:0000256|ARBA:ARBA00009405}.
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DR   EMBL; AL137605; CAB70838.1; -; mRNA.
DR   PIR; T46309; T46309.
DR   RefSeq; NP_001035865.1; NM_001042406.1.
DR   AlphaFoldDB; Q9NT06; -.
DR   PeptideAtlas; Q9NT06; -.
DR   DNASU; 54511; -.
DR   GeneID; 54511; -.
DR   CTD; 54511; -.
DR   PharmGKB; PA134989971; -.
DR   OrthoDB; 1210873at2759; -.
DR   UniPathway; UPA00896; UER00863.
DR   BioGRID-ORCS; 54511; 39 hits in 1138 CRISPR screens.
DR   GenomeRNAi; 54511; -.
DR   Genevisible; Q9NT06; HS.
DR   GO; GO:0004419; F:hydroxymethylglutaryl-CoA lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 2.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR043594; HMGL.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR42738:SF5; 3-HYDROXY-3-METHYLGLUTARYL-COA LYASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR42738; HYDROXYMETHYLGLUTARYL-COA LYASE; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
PE   2: Evidence at transcript level;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT   DOMAIN          54..100
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|Pfam:PF00682"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:CAB70838.1"
SQ   SEQUENCE   157 AA;  16899 MW;  474B780024CB85AC CRC64;
     AAPGSAMGNV PSAVKHCLSY QQLLREHLWI GDSVAGALDP AQETSQLSGL PEFVKIVEVG
     PRDGLQNEKV IVPTDIKIEF INRLSQTGLS VIEVTSFVSS RWVPQVAAGA TEISVFGAAS
     ESFSKKNINC SIEESMGKFE EVVKSARHMN IPARGYL
//