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Database: UniProt
Entry: Q9R233
LinkDB: Q9R233
Original site: Q9R233 
ID   TPSN_MOUSE              Reviewed;         465 AA.
AC   Q9R233; O70317; Q91YE0; Q91YE1; Q9QWT7; Q9Z1W3;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2003, sequence version 2.
DT   19-MAR-2014, entry version 116.
DE   RecName: Full=Tapasin;
DE            Short=TPN;
DE            Short=TPSN;
DE   AltName: Full=TAP-associated protein;
DE   AltName: Full=TAP-binding protein;
DE   Flags: Precursor;
GN   Name=Tapbp; Synonyms=Tapa;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
RC   TISSUE=Liver;
RX   PubMed=10085102; DOI=10.1074/jbc.274.13.8649;
RA   Li S., Paulsson K.M., Sjoegren H.-O., Wang P.;
RT   "Peptide-bound major histocompatibility complex class I molecules
RT   associate with tapasin before dissociation from transporter associated
RT   with antigen processing.";
RL   J. Biol. Chem. 274:8649-8654(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
RC   STRAIN=129;
RA   Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M.,
RA   Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L.;
RT   "Sequence of the mouse major histocomaptibility locus class II
RT   region.";
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
RX   PubMed=9716645; DOI=10.1007/s002510050430;
RA   Grandea A.G. III, Comber P.G., Wenderfer S.E., Schoenhals G.,
RA   Frueh K., Monaco J.J., Spies T.;
RT   "Sequence, linkage to H2-K, and function of mouse tapasin in MHC class
RT   I assembly.";
RL   Immunogenetics 48:260-265(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE (ISOFORM LONG).
RC   STRAIN=C57BL/10;
RX   PubMed=12095710; DOI=10.1016/S0165-2478(02)00104-9;
RA   Abarca-Heidemann K., Friederichs S., Klamp T., Boehm U.,
RA   Guethlein L.A., Ortmann B.;
RT   "Regulation of the expression of mouse TAP-associated glycoprotein
RT   (tapasin) by cytokines.";
RL   Immunol. Lett. 83:197-207(2002).
CC   -!- FUNCTION: Involved in the association of MHC class I with
CC       transporter associated with antigen processing (TAP) and in the
CC       assembly of MHC class I with peptide (peptide loading).
CC   -!- SUBUNIT: Interacts with TAP1 and is thus a subunit of the TAP
CC       complex. Interaction with TAP1 is TAP2 independent and is required
CC       for efficient peptide-TAP interaction. Obligatory mediator for the
CC       interaction between newly assembled MHC class I molecules,
CC       calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes
CC       bind to 1 TAP.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       type I membrane protein (Probable).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Long;
CC         IsoId=Q9R233-1; Sequence=Displayed;
CC       Name=Short;
CC         IsoId=Q9R233-2; Sequence=VSP_002578;
CC   -!- DOMAIN: The N-terminus is required for efficient association with
CC       MHC class I molecule and for a stable interaction between MHC I
CC       and calreticulin. Binding to TAP is mediated by the C-terminus
CC       region (By similarity).
CC   -!- SIMILARITY: Contains 1 Ig-like C1-type (immunoglobulin-like)
CC       domain.
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DR   EMBL; AF106278; AAD17964.1; -; mRNA.
DR   EMBL; AF110520; AAC97973.1; -; Genomic_DNA.
DR   EMBL; AF100956; AAC69893.1; -; Genomic_DNA.
DR   EMBL; AF043943; AAC61676.1; -; mRNA.
DR   EMBL; AJ316612; CAC69993.1; -; Genomic_DNA.
DR   EMBL; AJ316613; CAC70721.2; -; mRNA.
DR   RefSeq; NP_033344.1; NM_009318.2.
DR   UniGene; Mm.154457; -.
DR   ProteinModelPortal; Q9R233; -.
DR   SMR; Q9R233; 24-404.
DR   IntAct; Q9R233; 2.
DR   MINT; MINT-4138357; -.
DR   PhosphoSite; Q9R233; -.
DR   PaxDb; Q9R233; -.
DR   PRIDE; Q9R233; -.
DR   GeneID; 21356; -.
DR   KEGG; mmu:21356; -.
DR   UCSC; uc008cae.1; mouse. [Q9R233-1]
DR   CTD; 6892; -.
DR   MGI; MGI:1201689; Tapbp.
DR   eggNOG; NOG41000; -.
DR   HOGENOM; HOG000231214; -.
DR   HOVERGEN; HBG005156; -.
DR   InParanoid; Q9R233; -.
DR   KO; K08058; -.
DR   Reactome; REACT_102124; Immune System.
DR   ChiTaRS; TAPBP; mouse.
DR   NextBio; 300560; -.
DR   PRO; PR:Q9R233; -.
DR   ArrayExpress; Q9R233; -.
DR   Bgee; Q9R233; -.
DR   CleanEx; MM_TAPBP; -.
DR   Genevestigator; Q9R233; -.
DR   GO; GO:0000139; C:Golgi membrane; IEA:Ensembl.
DR   GO; GO:0005887; C:integral component of plasma membrane; TAS:MGI.
DR   GO; GO:0030670; C:phagocytic vesicle membrane; TAS:Reactome.
DR   GO; GO:0042825; C:TAP complex; IEA:Ensembl.
DR   GO; GO:0019885; P:antigen processing and presentation of endogenous peptide antigen via MHC class I; IEA:InterPro.
DR   GO; GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; IDA:MGI.
DR   GO; GO:0006952; P:defense response; IMP:MGI.
DR   GO; GO:0002397; P:MHC class I protein complex assembly; IEA:Ensembl.
DR   GO; GO:0050823; P:peptide antigen stabilization; IMP:UniProtKB.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003006; Ig/MHC_CS.
DR   InterPro; IPR003597; Ig_C1-set.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR008056; Tapasin.
DR   PANTHER; PTHR23411:SF0; PTHR23411:SF0; 1.
DR   Pfam; PF07654; C1-set; 1.
DR   PRINTS; PR01669; TAPASIN.
DR   SMART; SM00409; IG; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS00290; IG_MHC; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Disulfide bond;
KW   Endoplasmic reticulum; Glycoprotein; Immunoglobulin domain; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL        1     23       By similarity.
FT   CHAIN        24    465       Tapasin.
FT                                /FTId=PRO_0000014991.
FT   TOPO_DOM     24    416       Lumenal (Potential).
FT   TRANSMEM    417    437       Helical; (Potential).
FT   TOPO_DOM    438    465       Cytoplasmic (Potential).
FT   DOMAIN      295    402       Ig-like C1-type.
FT   COMPBIAS     37     40       Poly-Gly.
FT   SITE        431    431       May be involved in interaction with TAP.
FT   CARBOHYD    256    256       N-linked (GlcNAc...) (Potential).
FT   DISULFID     30     94       By similarity.
FT   DISULFID    118    118       Interchain (with C-57 in PDIA3) (By
FT                                similarity).
FT   DISULFID    318    385       By similarity.
FT   VAR_SEQ     407    436       Missing (in isoform Short).
FT                                /FTId=VSP_002578.
FT   CONFLICT     17     17       V -> F (in Ref. 3; AAC61676).
FT   CONFLICT    274    274       A -> G (in Ref. 3; AAC61676).
FT   CONFLICT    294    294       A -> G (in Ref. 3; AAC61676).
FT   CONFLICT    326    326       A -> S (in Ref. 1; AAD17964).
SQ   SEQUENCE   465 AA;  49736 MW;  DCADF445A1437C3C CRC64;
     MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL RHGPRGPPPR
     PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI PFPASAKWAR SLSPEQNCPR
     ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR EPVVITMATV VLTVLTHNPA PRVQLGKDAV
     LDLRFAYAPS ALEGSPSLDA GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA
     FAAWDDDEPW GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT
     PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT WLSTIRHHSD
     GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD VTLEVAGFSG PSIEDGIGLF
     LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA TAASLTIPRN SKKSQ
//
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