ID Q9R3C6_KLEPN Unreviewed; 157 AA.
AC Q9R3C6;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2000, sequence version 1.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=dihydrofolate reductase {ECO:0000256|ARBA:ARBA00012856};
DE EC=1.5.1.3 {ECO:0000256|ARBA:ARBA00012856};
GN Name=dfr17 {ECO:0000313|EMBL:AAF07904.1};
GN Synonyms=dfrA17 {ECO:0000313|EMBL:ACX50267.1};
GN ORFNames=GJJ08_027370 {ECO:0000313|EMBL:QOU54751.1}, pkp469IL_0365
GN {ECO:0000313|EMBL:AGP03339.1};
OS Klebsiella pneumoniae.
OG Plasmid pkp469IL {ECO:0000313|EMBL:AGP03339.1}, and
OG Plasmid unnamed2 {ECO:0000313|EMBL:QOU54751.1,
OG ECO:0000313|Proteomes:UP000439817}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573 {ECO:0000313|EMBL:AAF07904.1};
RN [1] {ECO:0000313|EMBL:AAF07904.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KpB35 {ECO:0000313|EMBL:AAF07904.1};
RA Kim J., Cho D.T., Seol S.Y., Lee J.C., Yoon J.J.;
RT "Characterization of novel two gene cassettes, dhfrXVII and aadA4.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AAF64678.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=11062188; DOI=10.1093/jac/46.5.703;
RA Naas T., Benaoudia F., Massuard S., Nordmann P.;
RT "Integron-located VEB-1 extended-spectrum beta-lactamase gene in a Proteus
RT mirabilis clinical isolate from Vietnam.";
RL J. Antimicrob. Chemother. 46:703-711(2000).
RN [3] {ECO:0000313|EMBL:AAF64678.1}
RP NUCLEOTIDE SEQUENCE.
RA Naas T.P., Benaoudia F., Massuard S., Nordmann P.;
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAF64678.1}
RP NUCLEOTIDE SEQUENCE.
RA Naas T., Benaoudia F., Massuard S., Nordmann P.;
RT "Characterization of two novel gene cassettes, dfr17 and aadA5 from
RT Klebsiella pneumoniae.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AAX98157.1}
RP NUCLEOTIDE SEQUENCE.
RA Gu B., Tong M., Liu G.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:ACX50267.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S-56 {ECO:0000313|EMBL:ACX50267.1};
RA Pryamchuk S.D., Fursova N.K., Abaev I.V., Pecherskikh E.I., Shishkova N.A.,
RA Korobova O.V.;
RT "Escherichia coli variable region of class 1 integron containing dfrA17 and
RT aadA5 cassettes.";
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:ADD97824.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Kp171 {ECO:0000313|EMBL:ADD97824.1};
RA Santos C., Caetano T., Ferreira S., Mendo S.;
RT "Class 1 integrons present in hospital environment.";
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:ADF59091.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NF200116 {ECO:0000313|EMBL:ADF59113.1}, NF805502
RC {ECO:0000313|EMBL:ADF59093.1}, NF806155 {ECO:0000313|EMBL:ADF59091.1},
RC NF811834 {ECO:0000313|EMBL:ACY68994.1}, and NF902382
RC {ECO:0000313|EMBL:ADF59101.1};
RA Wang F., Rui Y.;
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:AFM57740.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KP125 {ECO:0000313|EMBL:AFM57740.1};
RX PubMed=24098729;
RA Li B., Hu Y., Wang Q., Yi Y., Woo P.C., Jing H., Zhu B., Liu C.H.;
RT "Structural Diversity of Class 1 Integrons and Their Associated Gene
RT Cassettes in Klebsiella pneumoniae Isolates from a Hospital in China.";
RL PLoS ONE 8:E75805-E75805(2013).
RN [10] {ECO:0000313|EMBL:AGP03339.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pkp469IL {ECO:0000313|EMBL:AGP03339.1};
RA Chmelnitsky I., Shklyar M., Leavitt A., Sadovsky E., Navon-Venezia S.,
RA Edgar R., Carmeli Y.;
RT "KPC-2 encoding plasmids from Enterobacteriaceae in Israel.";
RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
RN [11] {ECO:0000313|EMBL:AHK14454.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B-811K {ECO:0000313|EMBL:AHK14454.1};
RA Astashkin E.I., Fursova N.K., Kartsev N.N.;
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
RN [12] {ECO:0000313|EMBL:AKL72212.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NF130174 {ECO:0000313|EMBL:AKL72212.1};
RA Lu W., Cheng C., Rui Y.;
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
RN [13] {ECO:0000313|EMBL:QOU54751.1, ECO:0000313|Proteomes:UP000439817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CriePir120 {ECO:0000313|EMBL:QOU54751.1,
RC ECO:0000313|Proteomes:UP000439817};
RC PLASMID=unnamed2 {ECO:0000313|EMBL:QOU54751.1,
RC ECO:0000313|Proteomes:UP000439817};
RX PubMed=32429555;
RA Shelenkov A., Mikhaylova Y., Yanushevich Y., Samoilov A., Petrova L.,
RA Fomina V., Gusarov V., Zamyatin M., Shagin D., Akimkin V.;
RT "Molecular Typing, Characterization of Antimicrobial Resistance, Virulence
RT Profiling and Analysis of Whole-Genome Sequence of Clinical Klebsiella
RT pneumoniae Isolates.";
RL Antibiotics 9:E261-E261(2020).
CC -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC reaction for de novo glycine and purine synthesis, and for DNA
CC precursor synthesis. {ECO:0000256|ARBA:ARBA00025067}.
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004903}.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00009539, ECO:0000256|RuleBase:RU004474}.
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DR EMBL; AF180469; AAF07904.1; -; Genomic_DNA.
DR EMBL; AF220757; AAF64678.1; -; Genomic_DNA.
DR EMBL; AY994155; AAX98157.1; -; Genomic_DNA.
DR EMBL; GQ896493; ACX50267.1; -; Genomic_DNA.
DR EMBL; GU120473; ACY68994.1; -; Genomic_DNA.
DR EMBL; GU590935; ADD97824.1; -; Genomic_DNA.
DR EMBL; GU944737; ADF59091.1; -; Genomic_DNA.
DR EMBL; GU944738; ADF59093.1; -; Genomic_DNA.
DR EMBL; GU944742; ADF59101.1; -; Genomic_DNA.
DR EMBL; GU944748; ADF59113.1; -; Genomic_DNA.
DR EMBL; JQ823009; AFM57740.1; -; Genomic_DNA.
DR EMBL; KF181262; AGP03339.1; -; Genomic_DNA.
DR EMBL; KF952266; AHK14454.1; -; Genomic_DNA.
DR EMBL; KP642030; AKL72212.1; -; Genomic_DNA.
DR EMBL; CP063010; QOU54751.1; -; Genomic_DNA.
DR RefSeq; WP_001389366.1; NZ_WUKO01000090.1.
DR AlphaFoldDB; Q9R3C6; -.
DR SMR; Q9R3C6; -.
DR GeneID; 83039323; -.
DR UniPathway; UPA00077; UER00158.
DR Proteomes; UP000439817; Plasmid unnamed2.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; Dihydrofolate Reductase, subunit A; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; DIHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR48069:SF3; DIHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR PRINTS; PR00070; DHFR.
DR SUPFAM; SSF53597; Dihydrofolate reductase-like; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Plasmid {ECO:0000313|EMBL:AGP03339.1}.
FT DOMAIN 2..156
FT /note="DHFR"
FT /evidence="ECO:0000259|PROSITE:PS51330"
SQ SEQUENCE 157 AA; 17559 MW; 8D44DC23D6C9050C CRC64;
MKISLISAVS ENGVIGSGPD IPWSVKGEQL LFKALTYNQW LLVGRKTFDS MGVLPNRKYA
VVSKNGISSS NENVLVFPSI ENALKELSKV TDHVYVSGGG QIYNSLIEKA DIIHLSTVHV
EVEGDIKFPI MPENFNLVFE QFFMSNINYT YQIWKKG
//
