ID QRT2_ARATH Reviewed; 439 AA.
AC Q9SFB7;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 01-MAY-2013, entry version 75.
DE RecName: Full=Polygalacturonase QRT2;
DE Short=AtQRT2;
DE Short=PG QRT2;
DE EC=3.2.1.15;
DE AltName: Full=Pectinase QRT2;
DE AltName: Full=Protein QUARTET 2;
DE Flags: Precursor;
GN Name=QRT2; OrderedLocusNames=At3g07970; ORFNames=F17A17.31;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
RA Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
RA Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
RA De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
RA Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
RA Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
RA Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
RA Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
RA Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
RA Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
RA Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
RA Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
RA Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
RA Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
RA Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
RA Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
RA Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
RA Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
RA Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
RA Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis
RT thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RG The Arabidopsis Information Resource (TAIR);
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND MUTAGENESIS OF VAL-372.
RX PubMed=8197459; DOI=10.1126/science.8197459;
RA Preuss D., Rhee S.Y., Davis R.W.;
RT "Tetrad analysis possible in Arabidopsis with mutation of the QUARTET
RT (QRT) genes.";
RL Science 264:1458-1460(1994).
RN [4]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RA Kim J., Patterson S.E.;
RT "Expression divergence and functional redundancy of polygalacturonases
RT in floral organ abscission.";
RL Plant Signal. Behav. 1:281-283(2006).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=17010199; DOI=10.1186/gb-2006-7-9-r87;
RA Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E.;
RT "Patterns of expansion and expression divergence in the plant
RT polygalacturonase gene family.";
RL Genome Biol. 7:R87.1-R87.14(2006).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17928369; DOI=10.1093/jxb/erm222;
RA Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A.;
RT "Expression of polygalacturonases and evidence to support their role
RT during cell separation processes in Arabidopsis thaliana.";
RL J. Exp. Bot. 58:3719-3730(2007).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19168715; DOI=10.1105/tpc.108.063768;
RA Ogawa M., Kay P., Wilson S., Swain S.M.;
RT "ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and
RT QUARTET2 are polygalacturonases required for cell separation during
RT reproductive development in Arabidopsis.";
RL Plant Cell 21:216-233(2009).
CC -!- FUNCTION: Polygalacturonase required for cell type-specific pectin
CC degradation to separate microspores. Involved in anther dehiscence
CC and floral organ abscission.
CC -!- CATALYTIC ACTIVITY: Random hydrolysis of (1->4)-alpha-D-
CC galactosiduronic linkages in pectate and other galacturonans.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall (By similarity).
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots with lower
CC expression levels in rosette leaves, flower buds and siliques.
CC Bearly detected in seeds. Found in flowers undergoing floral organ
CC abscission. Also expressed early in anther development, at the
CC time of microspore separation.
CC -!- DISRUPTION PHENOTYPE: The mature pollen grains are arranged in a
CC tetrad. No visible phenotype regarding floral organ abscission.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family.
CC -!- SIMILARITY: Contains 4 PbH1 repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF21207.1; Type=Erroneous gene model prediction;
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DR EMBL; AC013483; AAF21207.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74626.1; -; Genomic_DNA.
DR IPI; IPI00516594; -.
DR RefSeq; NP_187454.2; NM_111676.3.
DR UniGene; At.40276; -.
DR ProteinModelPortal; Q9SFB7; -.
DR SMR; Q9SFB7; 85-416.
DR STRING; 3702.AT3G07970.1-P; -.
DR CAZy; GH28; Glycoside Hydrolase Family 28.
DR EnsemblPlants; AT3G07970.1; AT3G07970.1; AT3G07970.
DR GeneID; 819988; -.
DR KEGG; ath:AT3G07970; -.
DR TAIR; At3g07970; -.
DR eggNOG; COG5434; -.
DR HOGENOM; HOG000237792; -.
DR InParanoid; Q9SFB7; -.
DR OMA; NIRLENA; -.
DR PhylomeDB; Q9SFB7; -.
DR ProtClustDB; CLSN2690525; -.
DR Genevestigator; Q9SFB7; -.
DR GO; GO:0005618; C:cell wall; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004650; F:polygalacturonase activity; IDA:TAIR.
DR GO; GO:0009901; P:anther dehiscence; IMP:TAIR.
DR GO; GO:0009830; P:cell wall modification involved in abscission; TAS:TAIR.
DR GO; GO:0010047; P:fruit dehiscence; IMP:TAIR.
DR GO; GO:0045490; P:pectin catabolic process; IMP:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:TAIR.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SMART; SM00710; PbH1; 4.
DR SUPFAM; SSF51126; Pectin_lyas_like; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Complete proteome;
KW Glycosidase; Hydrolase; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1 21 Potential.
FT CHAIN 22 439 Polygalacturonase QRT2.
FT /FTId=PRO_0000367915.
FT REPEAT 201 250 PbH1 1.
FT REPEAT 251 272 PbH1 2.
FT REPEAT 304 325 PbH1 3.
FT REPEAT 333 354 PbH1 4.
FT ACT_SITE 265 265 Proton donor (By similarity).
FT ACT_SITE 288 288 By similarity.
FT MUTAGEN 372 372 V->A: In qrt2-1; loss of function.
SQ SEQUENCE 439 AA; 48572 MW; D52F4691C95633A4 CRC64;
MYEKIIILSV FLLTFLPSCF SSYPFNHRDD LFMSSNVYYE TNRQHQHGHN TRNSHLKNRH
GYAPRSSPRS FNVNTFGAKA NGNDDSKAFM KAWEAACSST GIVYIVAPKN RDYMLKAVTF
SGPCKSSLII FKIYGRIEAW ENPSDYKERR HWIVFENVNN LRVEGGGRID GNGHIWWPKS
CKINPQLPCL GAPTAVTFVE CNNLRVSNIR LENAQQMHLT FQDCKNVKAL NLMVTSPADS
PNTDGIHVSG TQNILIQDSI VRTGDDCISI VSGSENVRAT GITCGPGHGI SIGSLGEDNS
EAYVSNVVVN KATLIGTTNG VRIKTWQGGH GMAKNIIFQD IIMKNVTNPI IINQDYCDRV
EACPEQKSAV QVSNVLYKNI QGTSSRPIAV KFVCSKNIPC RGISMQNVKL VDQTQQDVSK
ASCSNVKLDT RGNVSPLCT
//
