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Database: UniProt
Entry: Q9U2M4
LinkDB: Q9U2M4
Original site: Q9U2M4 
ID   HOT_CAEEL               Reviewed;         465 AA.
AC   Q9U2M4;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   19-MAR-2014, entry version 75.
DE   RecName: Full=Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial;
DE            Short=HOT;
DE            EC=1.1.99.24;
DE   Flags: Precursor;
GN   ORFNames=Y38F1A.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Catalyzes the cofactor-independent reversible oxidation
CC       of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA)
CC       coupled to reduction of 2-ketoglutarate (2-KG) to D-2-
CC       hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a
CC       substrate for HOT when using 2-KG as hydrogen acceptor, resulting
CC       in the formation of D-2-HG (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate =
CC       acetoacetate + (R)-2-hydroxyglutarate.
CC   -!- CATALYTIC ACTIVITY: 4-hydroxybutanoate + 2-oxoglutarate = succinic
CC       semialdehyde + (R)-2-hydroxyglutarate.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. Hydroxyacid-oxoacid transhydrogenase subfamily.
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DR   EMBL; AL032639; CAA21631.2; -; Genomic_DNA.
DR   PIR; E88343; E88343.
DR   PIR; T26686; T26686.
DR   RefSeq; NP_496764.1; NM_064363.4.
DR   UniGene; Cel.8339; -.
DR   ProteinModelPortal; Q9U2M4; -.
DR   SMR; Q9U2M4; 53-462.
DR   BioGrid; 40240; 1.
DR   DIP; DIP-24327N; -.
DR   MINT; MINT-1044715; -.
DR   STRING; 6239.Y38F1A.6.1; -.
DR   PaxDb; Q9U2M4; -.
DR   EnsemblMetazoa; Y38F1A.6.1; Y38F1A.6.1; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.2; Y38F1A.6.2; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.3; Y38F1A.6.3; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.4; Y38F1A.6.4; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.5; Y38F1A.6.5; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.6; Y38F1A.6.6; Y38F1A.6.
DR   EnsemblMetazoa; Y38F1A.6.7; Y38F1A.6.7; Y38F1A.6.
DR   GeneID; 174942; -.
DR   KEGG; cel:CELE_Y38F1A.6; -.
DR   UCSC; Y38F1A.6.1; c. elegans.
DR   CTD; 174942; -.
DR   WormBase; Y38F1A.6; CE24222; WBGene00012608; -.
DR   eggNOG; COG1454; -.
DR   GeneTree; ENSGT00390000003849; -.
DR   HOGENOM; HOG000243335; -.
DR   InParanoid; Q9U2M4; -.
DR   KO; K11173; -.
DR   OMA; RAACQCP; -.
DR   NextBio; 886136; -.
DR   PRO; PR:Q9U2M4; -.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0047988; F:hydroxyacid-oxoacid transhydrogenase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0015993; P:molecular hydrogen transport; ISS:UniProtKB.
DR   InterPro; IPR001670; ADH_Fe.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Mitochondrion; Oxidoreductase; Reference proteome;
KW   Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    465       Probable hydroxyacid-oxoacid
FT                                transhydrogenase, mitochondrial.
FT                                /FTId=PRO_0000324756.
SQ   SEQUENCE   465 AA;  50506 MW;  CB9A08D7376E2949 CRC64;
     MSASLARGIL SKMGGSCCPH HAPATNPFKL AKLHGNNKST DYAFEMVCST LRFGKGVTLE
     IGYDVRNLGA KKTLLITDKN VQNTIAFKNA EQALKMVNIE YEVFDDVLIE PTVNSMQKAI
     AFAKSKQFDS FIAVGGGSVI DTTKAAALYA SNPEADFLDF VGPPFGKSMQ PKNPMLPLIA
     VPTTAGTGSE TTAAAIMDLP EHKCKTGIRL RCIKPYLAVV DPLNVMSMPR NVAIYSGFDV
     LCHALESFTA LPFDQRSPRP ENPGVRPLYQ GSNPISDVWS KEALRIIGKY FRRSIFDPTD
     EEARTEMLKA SSFAGIGFGN AGVHLCHGLS YPISSQAKSC VADDYPKEKN LIPHGLSVMT
     TAVADFEFTT AACPDRHLIS AQTLGADIPN NASNEYISRT LCDRLRGYMR DFGVPNGLKG
     MGFEFSDIEM LTEAASHSVP NIAISPKSAD REIISTLYEK SLTVY
//
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