ID Q9U7D8_DROME Unreviewed; 853 AA.
AC Q9U7D8; Q9VSB7;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 200.
DE SubName: Full=FI23202p1 {ECO:0000313|EMBL:AHA95712.1};
DE SubName: Full=Pebble {ECO:0000313|EMBL:AAD52845.1, ECO:0000313|EMBL:AAF50508.2};
GN Name=pbl {ECO:0000313|EMBL:AAF50508.2,
GN ECO:0000313|FlyBase:FBgn0003041};
GN Synonyms=0083/20 {ECO:0000313|EMBL:AAF50508.2}, 0293/09
GN {ECO:0000313|EMBL:AAF50508.2}, 0542/03 {ECO:0000313|EMBL:AAF50508.2},
GN anon-WO0172774.22 {ECO:0000313|EMBL:AAF50508.2}, anon-WO0172774.25
GN {ECO:0000313|EMBL:AAF50508.2}, Dmel\CG8114
GN {ECO:0000313|EMBL:AAF50508.2}, Ect-2 {ECO:0000313|EMBL:AAF50508.2},
GN ECT2 {ECO:0000313|EMBL:AAF50508.2}, GEF {ECO:0000313|EMBL:AAF50508.2},
GN l(3)09645 {ECO:0000313|EMBL:AAF50508.2}, PBL
GN {ECO:0000313|EMBL:AAF50508.2}, Pbl {ECO:0000313|EMBL:AAF50508.2},
GN pbl-RA {ECO:0000313|EMBL:AHA95712.1}, Pbl/Ect2
GN {ECO:0000313|EMBL:AAF50508.2}, Peb {ECO:0000313|EMBL:AAF50508.2};
GN ORFNames=CG8114 {ECO:0000313|FlyBase:FBgn0003041}, Dmel_CG8114
GN {ECO:0000313|EMBL:AAF50508.2};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:AAD52845.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=10485851; DOI=10.1101/gad.13.17.2301;
RA Prokopenko S.N., Brumby A., O'Keefe L., Prior L., He Y., Saint R.,
RA Bellen H.J.;
RT "A putative exchange factor for Rho1 GTPase is required for initiation of
RT cytokinesis in Drosophila.";
RL Genes Dev. 13:2301-2314(1999).
RN [2] {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [4] {ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [6] {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [7] {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [8] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S.,
RA Svirskas R., Rubin G.;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [10] {ECO:0000313|EMBL:AAF50508.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
RN [11] {ECO:0000313|EMBL:AHA95712.1}
RP NUCLEOTIDE SEQUENCE.
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [12] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109357; DOI=.1534/g3.115.018929;
RG FlyBase Consortium;
RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E.,
RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M.,
RA Gelbart W.M., null;
RT "Gene Model Annotations for Drosophila melanogaster: Impact of High-
RT Throughput Data.";
RL G3 (Bethesda) 5:1721-1736(2015).
RN [13] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109356; DOI=.1534/g3.115.018937;
RG FlyBase Consortium;
RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E.,
RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M.,
RA null;
RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.";
RL G3 (Bethesda) 5:1737-1749(2015).
RN [14] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25589440;
RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E.,
RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M.,
RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M.,
RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M.,
RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M.,
RA Karpen G.H., Celniker S.E.;
RT "The Release 6 reference sequence of the Drosophila melanogaster genome.";
RL Genome Res. 25:445-458(2015).
RN [15] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RG Berkeley Drosophila Genome Project;
RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA Rubin G.;
RT "Drosophila melanogaster release 4 sequence.";
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
RN [16] {ECO:0000313|EMBL:AAF50508.2}
RP NUCLEOTIDE SEQUENCE.
RG FlyBase;
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF136492; AAD52845.1; -; mRNA.
DR EMBL; AE014296; AAF50508.2; -; Genomic_DNA.
DR EMBL; BT150408; AHA95712.1; -; mRNA.
DR RefSeq; NP_729306.1; NM_168241.3.
DR AlphaFoldDB; Q9U7D8; -.
DR SMR; Q9U7D8; -.
DR IntAct; Q9U7D8; 1.
DR DNASU; 38879; -.
DR EnsemblMetazoa; FBtr0076771; FBpp0076486; FBgn0003041.
DR GeneID; 38879; -.
DR UCSC; CG8114-RA; d. melanogaster.
DR AGR; FB:FBgn0003041; -.
DR CTD; 38879; -.
DR FlyBase; FBgn0003041; pbl.
DR VEuPathDB; VectorBase:FBgn0003041; -.
DR GeneTree; ENSGT00940000156299; -.
DR OrthoDB; 6929at2759; -.
DR BioGRID-ORCS; 38879; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38879; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003041; Expressed in cleaving embryo and 35 other cell types or tissues.
DR ExpressionAtlas; Q9U7D8; baseline and differential.
DR GO; GO:0005938; C:cell cortex; IDA:FlyBase.
DR GO; GO:0032154; C:cleavage furrow; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005096; F:GTPase activator activity; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:FlyBase.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IDA:FlyBase.
DR GO; GO:0030215; F:semaphorin receptor binding; IPI:FlyBase.
DR GO; GO:0031267; F:small GTPase binding; IPI:FlyBase.
DR GO; GO:0000915; P:actomyosin contractile ring assembly; IMP:FlyBase.
DR GO; GO:0007415; P:defasciculation of motor neuron axon; IMP:FlyBase.
DR GO; GO:0032507; P:maintenance of protein location in cell; IMP:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0007110; P:meiosis I cytokinesis; IMP:FlyBase.
DR GO; GO:0007111; P:meiosis II cytokinesis; IMP:FlyBase.
DR GO; GO:0007509; P:mesoderm migration involved in gastrulation; IMP:FlyBase.
DR GO; GO:0008078; P:mesodermal cell migration; IMP:FlyBase.
DR GO; GO:1903475; P:mitotic actomyosin contractile ring assembly; IMP:FlyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:FlyBase.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IMP:FlyBase.
DR GO; GO:0045792; P:negative regulation of cell size; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IMP:FlyBase.
DR GO; GO:0050770; P:regulation of axonogenesis; IGI:FlyBase.
DR GO; GO:0008360; P:regulation of cell shape; IMP:FlyBase.
DR GO; GO:2000431; P:regulation of cytokinesis, actomyosin contractile ring assembly; IEA:InterPro.
DR GO; GO:0051225; P:spindle assembly; IMP:FlyBase.
DR CDD; cd17732; BRCT_Ect2_rpt2; 1.
DR CDD; cd01229; PH_Ect2; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 3.40.50.10190; BRCT domain; 3.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR026817; Ect2.
DR InterPro; IPR049396; ECT2_BRCT0.
DR InterPro; IPR049395; ECT2_PH.
DR InterPro; IPR011993; PH-like_dom_sf.
DR PANTHER; PTHR16777; PROTEIN ECT2; 1.
DR PANTHER; PTHR16777:SF2; PROTEIN ECT2; 1.
DR Pfam; PF00533; BRCT; 1.
DR Pfam; PF21243; ECT2_BRCT0; 1.
DR Pfam; PF21242; ECT2_PH; 1.
DR Pfam; PF12738; PTCB-BRCT; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00292; BRCT; 2.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF52113; BRCT domain; 2.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS50172; BRCT; 2.
DR PROSITE; PS50010; DH_2; 1.
PE 2: Evidence at transcript level;
KW Reference proteome {ECO:0000313|Proteomes:UP000000803}.
FT DOMAIN 109..198
FT /note="BRCT"
FT /evidence="ECO:0000259|PROSITE:PS50172"
FT DOMAIN 204..282
FT /note="BRCT"
FT /evidence="ECO:0000259|PROSITE:PS50172"
FT DOMAIN 386..575
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
SQ SEQUENCE 853 AA; 96398 MW; 886C36C80AAB3AE1 CRC64;
MEMETIEEQS KCEMSITTLP TRICLVGGVG QDADTLQAAE SFGLPIVTSD TGLDILGESS
DWRTFYVLDD FEGASFEAIH KQKECILGPP ALKYAAEMKQ TLGQNSRPIY NYAMRGVVTC
FTGIRKKDEL TKLVNLIHSM GGCIKKDLNT KTTHLICNHS GGEKYQYAKT FRLTVVRPAW
VFAAWADRNS LEFDATQENF TKTHRLKAFE GQKICFFGFP VEEHQHMVDV LLENGGVCAE
LDDPECSHVV VDEHTTLTKP EPKNNHTHIL KSDWFWYTIQ NGYANEMDYL FGDYLDSITN
TPNTDRRDSL PISFNKRKRK RFSQRIQLEG TPLGSGKRRS SVSDAGLLSV SNSLFDCTTS
PDKLESDKLL HAEPEASDAT PAKKSMRFNH FMDFYTTESN YVGILDTILN LFKNKLEELA
ETNDPLLNKS EIKTIFGNFL PIHEVHQSML EHLRKLHANW REDCLIGDII IQHRDELIKA
YPPYVNFFEQ MKEQLQYCDR EYPRFHAFLK INQTKPECGR QGLQDLMIRP VQRLPSISLL
LNDILKHTTS GNADHGRLEE ALKAIKQVTL HINEDKRRTE SRMAIFDIFN DIEGCPAHLV
SSNRSFILKC EVNELSDSLS GRGDSLVLYL FSDSIELCKR RSKGFNTAKS PSTAKTHKHL
KLISLNTIRL VIDISDSPRA FGLLLRGDKE KLYTFTISDE ETDKSVYVKK LCNQIAAHTC
RTDADKLLIC RTSQELEVDI SDVNVSTLSK AFKLAAKTRL KVGRAFSFNK TPNKLKRAVS
TMMTSPFGST NSLTPASQLA QMRLASCTNI NEVDDEDCAS MRSSSPSTQS EMLVVPPLSV
QPTRKNKAVV GRI
//
