ID Q9V3H0_DROME Unreviewed; 450 AA.
AC Q9V3H0;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 184.
DE SubName: Full=DNAation factor-related protein 4 {ECO:0000313|EMBL:AAF53276.1};
DE SubName: Full=Drep4 {ECO:0000313|EMBL:AAF03220.2};
GN Name=Drep4 {ECO:0000313|EMBL:AAF53276.1,
GN ECO:0000313|FlyBase:FBgn0028406};
GN Synonyms=CAD {ECO:0000313|EMBL:AAF53276.1}, dCAD
GN {ECO:0000313|EMBL:AAF53276.1}, Dmel\CG9414
GN {ECO:0000313|EMBL:AAF53276.1}, DREP-4 {ECO:0000313|EMBL:AAF53276.1},
GN Drep-4 {ECO:0000313|EMBL:AAF53276.1}, DREP4
GN {ECO:0000313|EMBL:AAF53276.1}, drep4 {ECO:0000313|EMBL:AAF03220.2},
GN Rep4 {ECO:0000313|EMBL:AAF53276.1};
GN ORFNames=CG9414 {ECO:0000313|EMBL:AAF53276.1,
GN ECO:0000313|FlyBase:FBgn0028406}, Dmel_CG9414
GN {ECO:0000313|EMBL:AAF53276.1};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF03220.2};
RN [1] {ECO:0000313|EMBL:AAF03220.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=10627165; DOI=10.1038/sj.cdd.4400570;
RA Inohara N., Nunez G.;
RT "Genes with homology to DFF/CIDEs found in Drosophila melanogaster.";
RL Cell Death Differ. 6:823-824(1999).
RN [2] {ECO:0000313|EMBL:AAF53276.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [4] {ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [6] {ECO:0000313|EMBL:AAF53276.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [7] {ECO:0000313|EMBL:AAF53276.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [8] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S.,
RA Svirskas R., Rubin G.;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:AAF53276.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [10] {ECO:0000313|EMBL:AAF53276.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
RN [11] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109357; DOI=.1534/g3.115.018929;
RG FlyBase Consortium;
RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E.,
RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M.,
RA Gelbart W.M., null;
RT "Gene Model Annotations for Drosophila melanogaster: Impact of High-
RT Throughput Data.";
RL G3 (Bethesda) 5:1721-1736(2015).
RN [12] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109356; DOI=.1534/g3.115.018937;
RG FlyBase Consortium;
RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E.,
RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M.,
RA null;
RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.";
RL G3 (Bethesda) 5:1737-1749(2015).
RN [13] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25589440;
RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E.,
RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M.,
RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M.,
RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M.,
RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M.,
RA Karpen G.H., Celniker S.E.;
RT "The Release 6 reference sequence of the Drosophila melanogaster genome.";
RL Genome Res. 25:445-458(2015).
RN [14] {ECO:0007829|PDB:5XPC}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 39-130.
RX PubMed=28652364; DOI=10.1073/pnas.1705949114;
RA Choi J.Y., Qiao Q., Hong S.H., Kim C.M., Jeong J.H., Kim Y.G., Jung Y.K.,
RA Wu H., Park H.H.;
RT "CIDE domains form functionally important higher-order assemblies for DNA
RT fragmentation.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:7361-7366(2017).
RN [15] {ECO:0007829|PDB:5XPB}
RP X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 39-130.
RA Park H.H.;
RT "Crystal Structure of Selenomethionine labelled Drep4 CIDE domain.";
RL Submitted (JUN-2017) to the PDB data bank.
RN [16] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RG Berkeley Drosophila Genome Project;
RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA Rubin G.;
RT "Drosophila melanogaster release 4 sequence.";
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
RN [17] {ECO:0000313|EMBL:AAF53276.1}
RP NUCLEOTIDE SEQUENCE.
RG FlyBase;
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF149797; AAF03220.2; -; mRNA.
DR EMBL; AE014134; AAF53276.1; -; Genomic_DNA.
DR RefSeq; NP_609631.1; NM_135787.4.
DR PDB; 5XPB; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J=39-130.
DR PDB; 5XPC; X-ray; 1.90 A; A/B/C/D/E=39-130.
DR PDBsum; 5XPB; -.
DR PDBsum; 5XPC; -.
DR SMR; Q9V3H0; -.
DR IntAct; Q9V3H0; 3.
DR STRING; 7227.FBpp0080057; -.
DR PaxDb; 7227-FBpp0080057; -.
DR EnsemblMetazoa; FBtr0080478; FBpp0080057; FBgn0028406.
DR GeneID; 34734; -.
DR KEGG; dme:Dmel_CG9414; -.
DR UCSC; CG9414-RA; d. melanogaster.
DR AGR; FB:FBgn0028406; -.
DR CTD; 34734; -.
DR FlyBase; FBgn0028406; Drep4.
DR VEuPathDB; VectorBase:FBgn0028406; -.
DR eggNOG; ENOG502R0RF; Eukaryota.
DR GeneTree; ENSGT00390000014490; -.
DR HOGENOM; CLU_049235_0_0_1; -.
DR OMA; KAEHVEW; -.
DR OrthoDB; 5399497at2759; -.
DR Reactome; R-DME-140342; Apoptosis induced DNA fragmentation.
DR BioGRID-ORCS; 34734; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34734; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028406; Expressed in saliva-secreting gland and 22 other cell types or tissues.
DR ExpressionAtlas; Q9V3H0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004520; F:DNA endonuclease activity; IDA:FlyBase.
DR GO; GO:0004536; F:DNA nuclease activity; IBA:GO_Central.
DR GO; GO:0031491; F:nucleosome binding; IPI:FlyBase.
DR GO; GO:0006309; P:apoptotic DNA fragmentation; IDA:FlyBase.
DR CDD; cd01615; CIDE_N; 1.
DR Gene3D; 3.10.20.10; -; 1.
DR InterPro; IPR003508; CIDE-N_dom.
DR InterPro; IPR039729; DFF40.
DR InterPro; IPR015311; DFF40_C.
DR InterPro; IPR044925; His-Me_finger_sf.
DR PANTHER; PTHR13067; CASPASE-ACTIVATED DNASE; 1.
DR PANTHER; PTHR13067:SF2; CASPASE-ACTIVATED DNASE; 1.
DR Pfam; PF02017; CIDE-N; 1.
DR Pfam; PF09230; DFF40; 1.
DR SMART; SM00266; CAD; 1.
DR SUPFAM; SSF54277; CAD & PB1 domains; 1.
DR SUPFAM; SSF54060; His-Me finger endonucleases; 1.
DR PROSITE; PS51135; CIDE_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:5XPB, ECO:0007829|PDB:5XPC};
KW Apoptosis {ECO:0000256|PROSITE-ProRule:PRU00447};
KW Reference proteome {ECO:0000313|Proteomes:UP000000803}.
FT DOMAIN 46..121
FT /note="CIDE-N"
FT /evidence="ECO:0000259|PROSITE:PS51135"
FT REGION 12..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 450 AA; 52017 MW; CBAA690902178BAB CRC64;
MISYIRDAIM SPTTRSSSNN KKSSQPTGEV LKDVSSSESR GTAGAPMRGY KVTDNERTRK
YGIGANSLEM LIAKAKSKFP LLEPHLYLAS DGFEVSDDEY LKSLPAQTLF IVSGPDAVIT
TDADFEFEKM RQQSPLLKVA DIFYDFIEQH PEKFRRMITE YEHQKQRRVL DNSKTHLSLK
AEHVEWFTGG EERFHSKEEA MATRAQTRVR GYYYKAKEEL TRNPLYRQNA KARQVINSVL
EKFRYLLIGC DFFSMMFDRN CKQKHEFLKQ HLGDEETDAG RIPSKRLRQV IREYTKENCI
LDEWSTSLCS DLGDFYCQGS YSENGNSCSK QHTINPYASR ENLILFQVWN LDHQIELCRT
ILPALVANVE ELVSHPQTKC SIHKKQVVDI SVLEYFLEIF SLKNLKLVHI VCHEKAQRSN
RSNGRLLCSD CHEYRIVQEL MEVQVDSLAT
//
